2007 Fiscal Year Final Research Report Summary
The Japanese red pine genes exclusively expressed in a resistant family for pinewilt diseases
| Project/Area Number |
17380092
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
林学・森林工学
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| Research Institution | Kyoto University |
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Principal Investigator |
KURODA Hiroyuki Kyoto University, Research Institute of Sustainable Humanosphere, Lecturer (00115841)
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| Co-Investigator(Kenkyū-buntansha) |
KURODA Keiko Forestry and Forest Product Research Institute, Kansai Research Center, Group Leader (20353675)
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| Project Period (FY) |
2005 – 2007
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| Keywords | Pinus densiflora(Japanese red pine) / pine wild diseases / resistant family / Population fo Bursaphelenchus xylophilus (pine wood nematode) / interception of the worm migration / area of resin canals / differential gene expression / molecular markers for the resistance |
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| Research Abstract |
The seedlings obtained from resistant Pinus densiflora trees were inoculated with the pine wood nematode (Bursaphelenchus xylophilus). Distribution and population of the pine wood nematodes (PWN) in the stem tissue decreased in the resistant families from 22 days after the inoculation. This result suggested the migration and propagation of the PWN were blocked in the resistant seedlings.
PWN was inoculated on the cuttings obtained from resistant trees. Five days after the inoculation, PWN population in the xylem of the resistant trees was lower than that in the cortex. The xylem tissue may contribute to the resistance system in the initial stage of infection.
Differential expression libraries between the resistant and susceptible families of the conifer were constructed 13 days after the PWN inoculation. Among the ca. 200 million clones, the ca. 3000 colonies, showing distinct expression levels between the families, were one-path sequenced and clustered. The resulting 30 clones were 10 times higher or lower expression in a resistant family comparing to the susceptible one. The higher one contained enzymes, transporters and transcription factors involved in secondary metabolisms, suggesting the up-regulation in the resistant family after the PWN infection. The clones obtained here are the leading molecular markers for resistance against PWN.
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Research Products
(102 results)
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[Presentation] アカマツ林の健全性評価2008
Author(s)
黒田 宏之
Organizer
サイト型研究(滋賀サイト)研究会(口頭発表)
Place of Presentation
琵琶湖科学研究センター・大津市
Year and Date
2008-02-13
Description
「研究成果報告書概要(和文)」より
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[Book] Tree sap III(寺澤実編), Magnetic resonance micro-imaging of xylem sapdistribution in tree stems.2006
Author(s)
Kuroda, K., Ichihara, Y., Kanbara, Y., Inoue, T., Ogawa, A.
Total Pages
194(分担149-160)
Publisher
Hokkaido University Press
Description
「研究成果報告書概要(和文)」より
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