2007 Fiscal Year Final Research Report Summary
Research of tree pollen movement based on genotyping of single pollen grains
| Project/Area Number |
17380095
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
林学・森林工学
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| Research Institution | Kyoto University (2006-2007)
Hiroshima University (2005) |
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Principal Investigator |
ISAGI Yuji Kyoto University, Graduate SrhmlofAgricultme, Professor (50325130)
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| Co-Investigator(Kenkyū-buntansha) |
SUYAMA Yoshihisa Tohoku University, Graduate School of Agriculture, Associate Professor (60282315)
SHIBATA Mitsue Forestry and Foipa Products Research Institute, Tohoku Branch, Head of Research Team (10343807)
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| Project Period (FY) |
2005 – 2007
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| Keywords | pollination / genetic diversity / reproductive ecology |
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| Research Abstract |
The pollination process of tree species are important for the maintenance and regeneration of tree communities, and the genetic traits of pollen grains that are transported to flowers influence the reproduction and fitness of plants. Despite the importance of insect-pollination systems, understanding of those systems is still larking due to the absence of the genetic analysis of pollen grains. We extracted haploid nuclear DNA from a single pollen grain by using a simple pollen DNA extraction method. Multiple microsatellite genotypes of a single pollen, which are useful for ecological studies, e. g. pollen dispersal pattern, paternity analysis and evaluation of pollinators, were determined simultaneously within a single reaction tube. Using nine pairs of microsatellite primers, more than 90% of genotypes were successfully determined. Using the method developed for the present research, we evaluated the pollination efficiencies of bumblebees, flower beetles that visited the flowers of Magnolia obovata. Most of the pollen adhering to bumblebees was self-pollen. This result suggests that visitation by the insects may cause geitonogamous pollen flow and negatively affect the reproduction of M. obovata, causing inbreeding depression. In contrast, flower beetles transported large amounts of genetically diverse outcross pollen. The present results suggest that certain beetle species contribute to the pollination of M. obovata quantitatively and quantitatively. Direct gentic analysis of pollen grains will advance the understanding of plant mating systems, and may shed light on the mutualism and co-evolution of trees and flower visitors.
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[Journal Article] Genetic and reproductive consequences of forest fragmentation for populations of Magnolia obovata.2007
Author(s)
Isagi, Y., Tateno, R., Matsuki, Y., Hirao, A., Watanabe, S., and Shibata, M.
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Journal Title
Ecological Research 22
Pages: 382-389
Description
「研究成果報告書概要(和文)」より
Peer Reviewed
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[Journal Article] Isolation and characterization of microsatellite loci in Melicope quadrilocularis(Rutaceae), an endemic plant species of the Bonin Islands, Japan, and cross-species amplification in closely related taxa.2007
Author(s)
Katoh, S., Kaneko, S., Isagi, Y., Murakami, N., and Kato, H.
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Journal Title
Conservation Genetics 8
Pages: 1487-1490
Description
「研究成果報告書概要(和文)」より
Peer Reviewed
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[Journal Article] Genetic and reproductive consequences of forest fragmentation for populations of Magnolia obovata2007
Author(s)
Isagi, Y., Tateno, R., Matsuki, Y., Hirao, A., Watanabe, S., Shibata, M
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Journal Title
Ecological Research 22
Pages: 382-389
Description
「研究成果報告書概要(欧文)」より
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[Journal Article] Isolation and characterization of microsatellite loci in Melicope quadrilocularis (Rutaceae), an endemic plant species of the Bonin Islands, Japan, and cross-species amplification in closely related taxa2007
Author(s)
Katoh, S., Kaneko, S., Isagi, Y., Murakami, N., Kato, H
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Journal Title
Conservation Genetics 8
Pages: 1487-1490
Description
「研究成果報告書概要(欧文)」より
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[Journal Article] Microsatellite loci for a sparsely distributed species, Kalopanax pictus(Araliaceae)in Japanese temperate forests.2006
Author(s)
Fujimori, N., Kenta, T., Kondo, T., Kameyama, Y., Isagi, Y., and Nakashizuka, T.
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Journal Title
Molecular Ecology Notes 6
Pages: 468-469
Description
「研究成果報告書概要(和文)」より
Peer Reviewed
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[Journal Article] Reproductive success and distance to conspecific adults in the sparsely distributed tree Kalopanax pictus.2006
Author(s)
Fujimori, N., Samejima, H., Kenta, T., Ichie, T., Shibata, M., Iida, S., and Nakashizuka, T.
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Journal Title
Journal of Plant Research 119
Pages: 195-203
Description
「研究成果報告書概要(和文)」より
Peer Reviewed
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[Journal Article] Microsatellite loci for a sparsely distributed species, Kalopanax pictus (Araliaceae) in Japanese temperate forests2006
Author(s)
Fujimori, N., Kenta, T., Kondo, T., Kameyama, Y., Isagi, Y., Nakashizuka, T
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Journal Title
Molecular Ecology Notes 6
Pages: 468-469
Description
「研究成果報告書概要(欧文)」より
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[Journal Article] Reproductive success and distance to conspecific adults in the sparsely distributed tree Kalopanax pictus2006
Author(s)
Fujimori, N., Samejima, H., Kenta, T., Ichie, T., Shibata, M., lida, S., Nakashizuka, T
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Journal Title
Journal of Plant Research 119
Pages: 195-203
Description
「研究成果報告書概要(欧文)」より
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[Journal Article] Isolation and characterization of microsatellite loci in Shortia rotundifolia(Diapensiaceae),an endangered relict plant on the Ryukyu Islands and Taiwan.
Author(s)
Dan, T., Mitsui, Y., Ikeda, H., Isagi, Y., and Setoguchi, H.
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Journal Title
Conservation Genetics (In press)
Description
「研究成果報告書概要(和文)」より
Peer Reviewed
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[Journal Article] Effects of population density, sex morph, and tree size on reproduction in a heterodichogamous maple, Acer mono, in a temperate forest of Japan.
Author(s)
Shibata, M., Kikuchi S., Tanaka, H., Suweyoshi M., Yoshimaru H., and Niiyama K.
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Journal Title
Ecological Research (In press)
Description
「研究成果報告書概要(和文)」より
Peer Reviewed
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[Journal Article] Isolation and characterization of microsatellite loci in Shortia rotundifolia (Diapensiaceae), an endangered elict plant on the Ryukyu Islands and Taiwan
Author(s)
Dan, T., Mitsui, Y., Ikeda, H., Isagi, Y., Setoguchi, H
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Journal Title
Conservation Genetics (in press)
Description
「研究成果報告書概要(欧文)」より
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[Journal Article] Effects of population density, sex morph, and tree size on reproduction in a heterodichogamous maple, Acer mono, in a temperate forest of Japan
Author(s)
Shibata, M., Kikuchi, S., Tanaka, H., Suweyoshi, M., Yoshimaru, H., Niiyama, K
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Journal Title
Ecological Research (in press)
Description
「研究成果報告書概要(欧文)」より
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[Presentation] Comparison of pollen parents of seed set of Aesculus turbinata among fruits and individuals2008
Author(s)
Kawaguchi, Y., Sakurazawa, Y., Nanami, S., Matsuki, Y., Isagi, Y
Organizer
The 119th Annual Meeting of the Japanese Forest Society
Place of Presentation
Tokyo University of Agriculture and Technology, Fuchu campus
Year and Date
2008-03-27
Description
「研究成果報告書概要(欧文)」より
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[Presentation] Effect of eructation of Mt. Miyake on pollination system of Cameria japonica-direct genetic analysis of pollen grains transported by birds2008
Author(s)
Abe, H., Ueno, M., Yamamoto, Y., Suyama, Y., Tsumura, Y., Hasegawa, M.
Organizer
The 54th Annual Meeting of the Ecological Society of Japan
Place of Presentation
Fukuoka International Congress Center
Year and Date
2008-03-17
Description
「研究成果報告書概要(欧文)」より
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[Presentation] Clarification of pollination system in masting of tropical forest using the genetic analysis of insect adherent pollen grains2007
Author(s)
Kondo, T., Nishimura, S., Naito, Y., Tsumura, Y., Isagi, Y., Okuda, T., Leong, LS., Muhammad, N
Organizer
The 54th Annual Meeting of the Ecological Society of Japan
Place of Presentation
Ehime University
Year and Date
2007-03-20
Description
「研究成果報告書概要(欧文)」より
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[Presentation] Analysis of pollination efficiencies of insects that visited to Magnolia obovata using genetic analysis of pollen grain.2006
Author(s)
Matsuki, Y., Isagi, Y., Suyama, Y., Tateno, R., Shibata, M
Organizer
The 117th Annual Meeting of the Japanese Forest Society
Place of Presentation
Tokyo University of Agriculture, Setagaya campus
Year and Date
2006-04-03
Description
「研究成果報告書概要(欧文)」より
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[Book] Sustanability and Diversity of Forest Ecosystems -An Interdisciplinary Approach2008
Author(s)
Isagi, Y., Tateno, R., Matsuki, Y., Hirao, A., Watanabe, S., and Shibata, M.
Total Pages
86
Publisher
Springer
Description
「研究成果報告書概要(和文)」より
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[Book] Genetic and reproductive consequences of forest fragmentation for populations of Magnolia obovata2008
Author(s)
Isagi, Y., Tateno, R., Matsuki Y, Hirao, A., Watanabe, S., Shibata, M
Total Pages
86
Publisher
Nakashizuka, T. (ed.) Sustanability and Diversity of Forest Ecosystems -An Interdisciplinary Approach
Description
「研究成果報告書概要(欧文)」より
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[Book] Effects of human disturbance of forest on reproduction of a heterodichogamous maple, Acer mono2008
Author(s)
Shibata, M., Kikuchi, S., Tanaka, H., Yoshimaru, H., Niiyama, K
Total Pages
258
Publisher
In : M. Ichikawa, S Yamashita, T. Nakashizuka Sustainability and Biodiversity Assessment on Forest Utilization Options
Description
「研究成果報告書概要(欧文)」より
