2007 Fiscal Year Final Research Report Summary
Development of methodology for rhizosphere research
| Project/Area Number |
17380196
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Boundary agriculture
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| Research Institution | National Agricultural Research Organization |
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Principal Investigator |
TOSHIFUMI Murakami National Agricultural Research Organization, National Agricultural Research Center for Tohoku Region, Cover crop research team, Senior researcher (80391465)
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| Co-Investigator(Kenkyū-buntansha) |
OYANAGI Atsushi National Agriculture and Food Research Organization, National Institute of Crop Science, Noodle Whear Research team, Team leader (40355326)
NAKAMAOTO Tomomi The University of Tokyo, Graduate school of Agricultural and Life Sciences, Associate Professor (50180419)
SHIMANO Satoshi Miyagi University of Education, Environmental Education Center, Associate Professor (70355337)
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| Project Period (FY) |
2005 – 2007
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| Keywords | Root staining / Wheat / Growth angle of root / plowing / microcosm / DGGE / excess water tolerance / Ciliophora |
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| Research Abstract |
Root is a very important organ, which absorbs water and nutrients from soil, and is affected by the environment or soil organisms. However, the methods of research have not yet been developed well, and the ecology of rhizosphere is still obscure. Our project aimed at development of methods of root and soil organisms.
The root staining method by pressure-injection of dye for field grown plant was developed. By using this method, relationship between roots of neighboring plants of field grown tomato was clarified. The method was modified for monocotyledonous plant ; the leaf sheath near the ground was filled with resin.
The experimental model system of wet injury (puddling and leveling method) was developed to know the genetic characteristics of wheat root under excess water condition in soil. It was clarified that the tolerance of water injury of Mizutakamoji (Agropyron humidum) and rye were higher than wheat.
A sampling method was developed to know the vertical and horizontal distribution of soil organisms and factors controlling them. Roots enhanoed the activity of soil organisms more in a tilled plot than that in a non-tilled plot. The microcosm method using a litter bag was developed for evaluating the effect of soil organisms on the decomposition of organic matter and nutrient absorption by roots.
The PCR-DGGE method for analyzing community structure of soil ciliate was developed. The method can detect more species in soil compared to miauscopic method.
The ecology of soil ciliate, predator of bacteria, was investigated by molecular biological method based on environmental DNA for analyzing community structure of bacteria.
Our developed methods for root system and soil organisms would contribute to clarify the interaction between root and soil organisms.
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Research Products
(53 results)
