2007 Fiscal Year Final Research Report Summary
Identification and fimoticual analyses ofglycoproteins responsible for sperm-egg interaction in birds
| Project/Area Number |
17380200
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Applied molecular and cellular biology
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| Research Institution | Nagoya University |
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Principal Investigator |
MATSUDA Tsukasa Nagoya University, Graduate School of Bioagricultural Sciences, Professor (20144131)
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| Co-Investigator(Kenkyū-buntansha) |
NADANO Daita Nagoya University, Graduate Schcol of Bioagricultural Sciences, Asscoiate Professor (00228074)
SATO Chihiro Nagoya University, Bioscience and Biattchnology Center, Professor (10343211)
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| Project Period (FY) |
2005 – 2007
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| Keywords | Fertilization / protein / sugar chains / development / differentiation / applied animal science |
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| Research Abstract |
From a viewpoint of cell-cell interaction, Structural and functional analyses have been done on two kinds of glycoproteins (ZP glycoprotein of zona pellucid and phospholipid-binding protein, MFG-ES, of sperm surface),which have been suggested to be involved in specific recognition and binding for gamete interaction. As for the ZP glycoprotein, ligand blotting and immuniprecipitation analyses demonstrated that ZPB1 specifically bound to ZPC and thereby disulfide-bridged ZPB1 dimmerization was induced. Such ZPC-induced ZPB1 conformational change was suggested to be responsible for acquisition of sperm activation ability. Furthermore, ZPB1 interacted with ZPC secreted from transfected COS cells and formed insoluble matrixes with ZPC on the cell surface. In vitro incubation of ZPB1 with ZPC spontaneously produced fibrous aggregates of ZPB1-ZPC hetero complexes, which were visible under optical microscopy and morphologically resembled the aggregates obtained from mechanically decomposed chi
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cken egg-envelope. Formation of such fibrous aggregates depended on ZPC/ZPB1 ratio, and involved ZPB1 dimerization through disulfide cross-linking, which had been found in authentic egg-envelope developed in hen's ovary. Furthermore, addition of excessive amounts of ZPC to ZPB1 produced soluble but high molecular weight heterocomplexes with increased adherence property against polystyrene ELISA plates. Thus, the specific association between ZPB1 and ZPC could play pivotal roles to initiate complex formation of hetero-polymers of ZP proteins in egg-envelope matrix construction. As for MFG-E8, this phospholipid-binding protein was shown to be expressed in various types of cells other than sperm by mRNA analyses of various animal cells and tissues, and some important roles in the differentiation and function of adipocytes and the apoptosis of mammary epithelial cells were suggested as a membrane protein on outer surface of microvesicles, termed exosome. Presence of MFG-ES on exosomes suggested contribution of membrane microvesicles to sperm-egg interaction and importance of complex supermolecular interaction among ZP-glycoproteins and the membrane-vesicle constituent molecules, including MFG-E8. Less
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Research Products
(24 results)
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[Journal Article] Protein-bound 4-Hydroxy-2-nonenal : AN ENDOGENOUS TRIGGERING ANTIGEN OF ANTI-DNA R.ESPONSE.2007
Author(s)
Toyoda, K, Nagae, R, Akagawa, M, Ishino, K, Shibata, T, Ito, S, Shibata, N, Yamamoto, T, Kobayashi, M, Takasaki, Y, Matsuda, T, Uchida, K
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Journal Title
J Biol Chem. Aug 31 282(35)
Pages: 25769-78
Description
「研究成果報告書概要(欧文)」より
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[Journal Article] Identification and characterization of microvesicles secreted by 3T3-L1 adipocytes : redox- and hormone-dependent induction of milk fat globule-epidermal growth factor 8-associated microvesicles.2007
Author(s)
Aoki, N, Jin-no, S, Nakagawa, Y, Asai, N, Arakawa, E, Tamura, N, Tamura, T. Matsuda, T
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Journal Title
Endocrinology. Aug 148(8)
Pages: 3850-62
Description
「研究成果報告書概要(欧文)」より
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[Journal Article] Involvement of the alpha2,8-polysialyltransferases D/STX and IV/PST in the biosynthesis of polysialic acid chains on the 0-linked glycoproteins in rainbow trout ovary.2006
Author(s)
Asahina, S, Sato, C, Matsuno, M, Matsuda, T. Colley, K Kitaiima, K
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Journal Title
J Biochem (Tokyo). Nov 140(5)
Pages: 687-701
Description
「研究成果報告書概要(欧文)」より
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[Journal Article] Weaning-induced expression of a milk-fat globule protein, MFG-E8, in mouse mammary glands, as demonstrated by the analyses of its mRNA, protein and phosphatidylserine-binding activity.2006
Author(s)
Nakatani, H, Aoki, N, Nakagawa, Y, Jin-No, S, Aoyama, K, Oshima, K, Ohira, S, Sato, C, Nadano, D, Matsuda, T
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Journal Title
Biochem J 395
Pages: 21-30
Description
「研究成果報告書概要(欧文)」より
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[Presentation] Functional analysis of apoptosis-relating protein, MFG-E8, in involuting mammary glands2006
Author(s)
Nakatani, H, Aoki, N, Okajima, T, Nadano, D, Matsuda, T
Organizer
Chyubu-blanch Meeting of apanese Biochemical Society
Place of Presentation
Nagoya
Year and Date
20060500
Description
「研究成果報告書概要(欧文)」より
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[Presentation] Okumura, H, Aoki, N, Sato, C, Okajima, T, Nadano, D, Matsuda, T2006
Author(s)
Okumura, H, Aoki, N, Sato, C, Okajima, T, Nadano, D, Matsuda, T
Organizer
Chyubu-blanch Meeting of apanese Biochemical Society
Place of Presentation
Nagoya
Year and Date
20060500
Description
「研究成果報告書概要(欧文)」より
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