Studies on the mechanism for HIV-1 Thai CRF01_AE-induced high apoptosis induction and the development of its control

2008 Fiscal Year Final Research Report

• Project/Area Number: 17390135
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Virology
• Research Institution: Osaka University
• Principal Investigator: IKUTA Kazuyoshi Osaka University, 微生物病研究所, 教授 (60127181)
• Co-Investigator(Kenkyū-buntansha): SAKUDO Akikazu 大阪大学, 微生物病研究所, 助教 (10397672) ; KAMEOKA Masanori 大阪大学, 微生物病研究所, 特任准教授 (60281838) ; SHOJI Sanae 大阪大学, 微生物病研究所, 特任研究員 (20397681)
• Project Period (FY): 2005 – 2008
• Keywords: HIV / AIDS / CRF01_AE / アポトーシス / 重感染 / 組換え型 / ワクチン

Research Abstract

HIV-1は遺伝子変異を引き起こしやすく、これがHIV-1病態の大きな特徴となっており、根本的な治療法やワクチン開発ができない原因となっている。本研究では、HIV-1の病態機序解明およびglobal epitope誘導型ワクチン開発を目指して、タイ型(AE型)と欧米型(B型)間のウイルス学的な差異、組換え型出現機序、Env gp41を認識させやすいHIV-1の構築などにフォーカスした研究を行なった。

Research Products

• [Journal Article] Phenotypic studies on recombinant human immunodeficiency virus type 1 (HIV-1) containing CRF01_AE env gene derived from HIV-1-infected patient (2009)
  • Author(s): Utachee, P., Jinnopat, P., Isarangkura-Na-Ayuthaya, P., de Silva, U.C., Nakamura, S., Siripanyaphinyo, U., Wichukchinda, N., Tokunaga, K., Yasunaga, T., Sawanpanyalert, P., Ikuta, K., Auwanit, W., and Kameoka, M.
  • Journal Title: residing central Thailand. Microbes Infect 11 Pages: 2334-343
• [Journal Article] Inhibitory function of adapter-related protein complex 2 alpha 1 subunit in the process of nuclear translocation of human immunodeficiency virus type 1 genome (2008)
  • Author(s): Kitagawa, Y., Kameoka, M., Shoji-Kawata, S., Iwabu, Y., Mizuta, H., Tokunaga, K., Fujino, M., Natori, Y., Yura, Y., and Ikuta, K.
  • Journal Title: Virology 373 Pages: 171-180
• [Journal Article] Superinfection of defective human immunodeficiency virus type 1 with different subtypes of wild-type virus efficiently produces infectious variants with the initial viral phenotypes by complementation followed by recombination (2008)
  • Author(s): Iwabu, Y., Mizuta, H., Kawase, M., Kameoka, M., Goto, T., and Ikuta, K.
  • Journal Title: Microbes Infect 10 Pages: 504-513
• [Journal Article] An HIV clade B defective particle producing cell line (L-2) shows stability in its HIV mutated genome over 10 years (2008)
  • Author(s): Zhong, Q., Ikuta, K., and Luftig, R. B.
  • Journal Title: Current Topics in Virology 6 Pages: 15-24
• [Journal Article] The RING finger ubiquitin ligase RNF125/TRAC-1 down-modulates HIV-1 replication in primary human peripheral blood mononuclear cells (2007)
  • Author(s): Shoji-Kawata, S., Zhong, Q., Kameoka, M., Iwabu, Y., Sapsutthipas, S., Luftig, R.B., and Ikuta, K.
  • Journal Title: Virology 368 Pages: 191-204
• [Journal Article] Superinfection of human immunodeficiency virus type 1 (HIV-1) to cell clone persistently infected with defective virus induces production of highly cytopathogenic HIV-1 (2006)
  • Author(s): Iwabu, Y., Goto, T., Tsuji, S., Warachit, J., Li, G.M., Shoji, S., Kameoka, M., Ikuta, K.
  • Journal Title: Microbes Infect 8 Pages: 1773-1782
• [Journal Article] Higher frequency of premature stop codon mutations at vpu gene of human immunodeficiency virus type 1 CRF01_AE compared with those of other subtypes (2005)
  • Author(s): Komoto, S., Tsuji, S., Lee, B.J., Iwabu, Y., Kojima, Y., Otake, T., Taniguchi, K., Ikuta, K.
  • Journal Title: Microbes Infect 7 Pages: 139-147
• [Journal Article] Different susceptibility of human immunodeficiency virus type 1 to Env gp41-derived synthetic peptides corresponding to the two heptad repeat regions (2005)
  • Author(s): Isarangkura, P.N.A., Li, G.M., Warachit, J., Iwabu, Y., Tsuji, S., Auwanit, W., Yamamoto, D., Goto, T., Hayashi, Y., Kiso, Y., Ikuta. K.
  • Journal Title: Microbes Infect 7 Pages: 356-364
• [Journal Article] Interleukin-4 upregulates T-tropic human immunodeficiency virus type 1 transcription in primary CD4^+ CD38^+ T-lymphocyte subset (2005)
  • Author(s): Li, Y.G., Iwabu, Y., Warachit, J., Kinomoto, M., Ibrahim, M.S., Tsuji, S., Mukai, T., Kameoka, M., Tokunaga, K., Sata, T., Ikuta, K.
  • Journal Title: Microbiol. Immunol 49 Pages: 155-165
• [Journal Article] Amino acid 36 in HIV-1 gp41 ectodomain control fusogenic activity : Implications for the molecular mechanism of viral escape from a fusion inhibitor (2005)
  • Author(s): Kinomoto, M., Yokoyama, M., Sato, H., Kojima, A., Kurata, T., Ikuta, K., Sata, T.,Tokunaga, K.
  • Journal Title: J. Virol 79 Pages: 5996-6003
• [Presentation] CRF01_AE 型Gagがプロテアーゼ阻害剤に対するウイルスの薬剤感受性を低下させる分子機構について (2008)
  • Author(s): 亀岡正典、Piyamat Jinnopat 、Panasda Isarangkura-N-A、Piraporn Utachee、北川友紀子、Udayanga Chandimal de Silva 、Uamporn Siripanyaphinyo、亀岡陽子、徳永研三、Pathom Sawanpanyalert 、WattanaAuwanit、生田和良
  • Organizer: 第56回日本ウイルス学会学術集会
  • Place of Presentation: 岡山
  • Year and Date: 20081026-28
• [Presentation] 高細胞融合能を示す非感染性HIV-1粒子のEnv gp41へのアミノ酸変異導入の効果 (2008)
  • Author(s): 川瀬倫子、水田浩之、川下理日人、鷹岡昭夫、長谷川紀昭、山田直子、生田和良
  • Organizer: 第56回日本ウイルス学会学術集会
  • Place of Presentation: 岡山
  • Year and Date: 20081026-28
• [Presentation] HIV-1複製関連宿主因子として同定されたAP2αのウイルス増殖抑制機序の解析 (2007)
  • Author(s): 北川友紀子、亀岡正典、水田浩之、小路早苗、生田和良
  • Organizer: 第55回日本ウイルス学会学術集会
  • Place of Presentation: 札幌
  • Year and Date: 20071021-23
• [Presentation] HIV-1持続感染細胞への重感染により出現する組換えHIV-1 (2007)
  • Author(s): 水田浩之、川瀬倫子、岩部幸枝、亀岡正典、後藤俊幸、生田和良
  • Organizer: 第55回日本ウイルス学会学術集会
  • Place of Presentation: 札幌
  • Year and Date: 20071021-23
• [Presentation] タイ国サラブリー県の薬剤未治療HIV-1感染者から分離したHIV-1プロテアーゼの遺伝子型および表現型の解析 (2007)
  • Author(s): 亀岡正典、北川友紀子、川下理日人、徳永研三、生田和良
  • Organizer: 第55回日本ウイルス学会学術集会
  • Place of Presentation: 札幌
  • Year and Date: 20071021-23
• [Presentation] Distinctive Biological Features of HIV Type 1 Subtype C Isolates of Indian Origin : Implications for Disease Progression (2007)
  • Author(s): Arias Juan Fernando、生田和良
  • Organizer: 第55回日本ウイルス学会学術集会
  • Place of Presentation: 札幌
  • Year and Date: 20071021-23
• [Book] 医科ウイルス学(高田賢蔵)(レトロウイルス科) (2009)
  • Author(s): 生田和良
  • Publisher: 南江堂
• [Patent(Industrial Property Rights)] HIV転写制御因子
  • Inventor(s): 生田和良、小路早苗