2006 Fiscal Year Final Research Report Summary
Roles of mast cells on viral infection and mast cell-based immunotherapy
| Project/Area Number |
17390310
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Dermatology
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| Research Institution | University of Yamanashi |
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Principal Investigator |
SHIMADA Shinji University of Yamanashi, Department of Research Interdisciplinary Graduate School of Medicine and Engineering, Professor, 大学院医学工学総合研究部, 教授 (10114505)
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| Co-Investigator(Kenkyū-buntansha) |
MATSUE Hiroyuki Chiba University, Graduate School of Medicine, Professor, 医学(系)研究科(研究院), 教授 (10250424)
SHIBAGAKI Naotaka University of Yamanashi, Department of Research Interdisciplinary Graduate School of Medicine and Engineering, Associate Professor, 大学院医学工学総合研究部, 助教授 (40262662)
NAGASAKA Akiko University of Yamanashi, University of Yamanashi Hospital, Resiearch Associafe, 大学院医学部附属病院, 助手 (60377546)
AOKI Rui University of Yamanashi, University of Yamanashi Hospital, Instructor, 大学院医学部附属病, 医員 (10377541)
NISHIYAMA Hiroyuki Nagoya University, School of Medicine, Professor, 大学院医学系研究科, 教授 (60115615)
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| Project Period (FY) |
2005 – 2006
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| Keywords | Mast cell / Herpes simplex virus / Toll-like receptor / Innate immunity / Acquired immunity |
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| Research Abstract |
Mast cells have long been considered as major effector cells in type I-allergic responses. Recent studies, however, have revealed the additional importance of mast cells in host defense against pathogens, especially bacteria. Although we have recently reported that cutaneous mast cells express functional Toll-like receptors (TLRs) that recognize viral components, it remains largely unknown whether mast cells are involved in host defense against viruses. We hypothesized that degranulation and inflammatory cytokine production by mast cells may be induced upon herpes simplex virus (HSV) infection, thus being involved in the inflammatory and immune responses against the virus. To test this, we used murine fetal skin-derived cultured mast cells (FSMC). Rapid (within 10 min) degranulation by FSMC was induced in a virus number-dependent fashion without affecting cell viability, when HSVwas added to FSMC cultures (β-hexosaminidase assay). The viral components were detected within 24 h after the infection (immunohistochemistry). In addition, inflammatory cytokines (i.e., TNFα, IL-6, and IFNβ) were not induced in the culture supernatants 24 h post infection. Upon inoculation of HSV (5 x 10^5 PFU) intradermally into BALB/c mice, the dermal infiltrates and significant increase in the number of degranulated mast cells were observed within 2 h (toluidine blue staining). These results indicate that cutaneous mast cells degranulate without elaborating inflammatory cytokines upon HSV infection, thus participating in the induction of inflammatory responses and the subsequent acquired immune responses against the virus.
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Research Products
(22 results)
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[Journal Article] CD4^+ CD25^<high> regulatory T cells are markedly decreased in blood of patients with pemphigus vulgaris.2007
Author(s)
Sugiyama, H., Matsue, H., Nagasaka, A., Nakamura, Y., Tsukamoto, K., Shibagaki, N., Kawamura, T., Kitamura, R., Ando, N., Shimada, S.
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Journal Title
Dermatology 214
Pages: 210-220
Description
「研究成果報告書概要(欧文)」より
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[Journal Article] Human eosinophils have an intact Smad signaling pathway leading to a major transforming growth factor-β target gene expression.2007
Author(s)
Kanzaki, M., Shibagaki, N., Hatsushika, K., Mitsui, H., Inozume, T., Okamoto, A., Dobashi, Y., Ogawa, H., Shimada, S., Nakao, A.
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Journal Title
Int Arch Allergy Immunol 142
Pages: 309-317
Description
「研究成果報告書概要(欧文)」より
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[Journal Article] Gap junction-mediated intercellular communication between dendritic cells (DCs) is required for effective activation of DCs.2006
Author(s)
Matsue, H., Yao, J., Matsue, K., Nagasaka, A., Sugiyama, H., Aoki, R., Kitamura, M., Shimada, S.
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Journal Title
J. Immunol. 176
Pages: 181-190
Description
「研究成果報告書概要(欧文)」より
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[Journal Article] Carbonic anhydrase is a tumor vessel endothelium-associated antigen targeted by dendritic cell therapy.2005
Author(s)
Yoshiura, K., Nakaoka, T., Nishishita, T., Sato, K., Yamamoto, A., Shimada, S., Saida, T., Kawakami, Y., Takahash, T., Fukuda, H., Imajoh-Ohmi, S., Oyaizu, N., Yamashita, N.
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Journal Title
Clinical Cancer Res. 11
Pages: 8201-8207
Description
「研究成果報告書概要(欧文)」より
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[Journal Article] Intratumoral injection of immature dendritic cells enhances antitumor effect of hyperthermia using magnetic nanoparticles.2005
Author(s)
Tanaka, K., Kobayashi, T., Kawamura, T., Shimada, S., Matsumoto, K., Saida, T.
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Journal Title
Int J Cancer 116
Pages: 624-633
Description
「研究成果報告書概要(欧文)」より
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[Journal Article] Novel melanoma antigen, FCRL/FREB, identified by cDNA profile comparison using DNA chip are immunogenic in multiple melanoma patients.2005
Author(s)
Inozume, T., Matsuzaki, Y., Kurihara, S.Fujita, T., Yamamoto, A., Aburatani, H., Shimada, S., Kawakami, Y.
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Journal Title
Int J Cancer 114
Pages: 283-290
Description
「研究成果報告書概要(欧文)」より
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[Journal Article] Heat immunotherapy using magnetic nanoparticles and dendritic cells for T-Lymphoma2005
Author(s)
Tanaka, K., Ito, A., Kobayashi, T., Kawamura, T., Shimada, S., Matsumoto, K., Saida, T., Honda, H.
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Journal Title
Biosci ＆ Bioeng 100
Pages: 112-115
Description
「研究成果報告書概要(欧文)」より
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[Journal Article] Dysfunctional blood and target tissue CD4^+ CD25^<high> regulatory T cells in psoriasis : Mechanism underlying unrestrained pathogenic effector T Cell proliferation2005
Author(s)
Sugiyama, H., Gyulai, R., Toichi, E., Garaczi, E., Shimada, S., Stevens, SR., McCormick, TS., Cooper, KD.
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Journal Title
J Immunol. 174
Pages: 164-173
Description
「研究成果報告書概要(欧文)」より
