2006 Fiscal Year Final Research Report Summary
Minimally-immunogenic Decellularized Porcine Valve Provides In-situ Recellularization in a Stentless Bioprosthetic Valve
| Project/Area Number |
17390380
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Thoracic surgery
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| Research Institution | OSAKA UNIVERSITY |
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Principal Investigator |
ICHIKAWA Hajime Osaka University, Graduate School of Medicine, Professor, 医学系研究科, 特任教授(常勤) (60303939)
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| Co-Investigator(Kenkyū-buntansha) |
MATSUDA Hikaru Osaka University, Professor Eneritus, 名誉教授 (00028614)
SAWA Yoshiki Osaka University, Graduate School of Medicine, Professor, 医学系研究科, 教授 (00243220)
FUKUSHIMA Norihide Osaka University, Graduate School of Medicine, Associate Professor, 医学系研究科, 助教授 (30263247)
MATSUSHIMA Goro Osaka University, Graduate School of Medicine, Associate Professor, 医学系研究科, 助教授 (20314312)
NISHIMURA Motonobu Tottori University, Faculty of Medicine, Professor, 医学部, 教授 (90291442)
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| Project Period (FY) |
2005 – 2006
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| Keywords | Tissue Engineering / Tissue Engineering heart valve / Prosthetic heart valve / Decellularization |
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| Research Abstract |
CurRently used bioprosthetic valves have limitations such as.calcification and. functional deterioration, and revitalization through cellular ingrowth is impossible. To overcome these obstacles, we have developed a minimally immunogenic tissue-engineered valve that consists of an unfixed, decellularized porcine valve scaffold capable of being spontaneously revitalized in vivo after implantation. Porcine aortic root tissue was decellularized using detergents. Histological examination showed that the newly developed detergent treatment effectively removed cellular debris from the porcine aortic tissue. In a preliminary study, Decellularized tissue implanted subdermally in rats for 14 and 60 days showed minimal inflammatory cell infiltration and calcification. As an in vivo valve replacement model, the decellularized porcine valve was implanted in the pulmonary valve position in dogs. In this series, spontaneous reendothelialization and repopulation of the medial cells were observed within 2 months, and good valve function without regurgitation was observed by echocardiography up to 6 months. The minimally immunogenic decellularized porcine valve proved effective in mitigating postimplant calcification and provided a suitable matrix for revitalizing prostheses through in situ recellularization, cellular ingrowth, and tissue remodeling. We also find that our decellularized valve containing a fusion protein combined fibronectin and hepatocyte growth factor might accelerate in situ recellularization.
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