2007 Fiscal Year Final Research Report Summary
Mechanical stress-generated expression of MMPs in human PDL cell
| Project/Area Number |
17390557
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Orthodontic/Pediatric dentistry
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| Research Institution | Osaka University (2007)
Matsumoto Dental University (2005-2006) |
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Principal Investigator |
UEMATSU Setsuko Osaka University, Dental Hospital, Senior lecturer (80271378)
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| Co-Investigator(Kenkyū-buntansha) |
HIRAOKA Yukihiro Matsumoto Dental University, Graduate School, Professor (20097512)
UEMATSU Takashi Matsumoto Dental University, Graduate School, Associate professor (40203476)
USUI Yohei Matsumoto Dental University, School ofDentistry, Assistant professor (00387424)
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| Project Period (FY) |
2005 – 2007
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| Keywords | IL-17 / T cell / periodontal ligament cell / MMP-1 / mechanical stress |
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| Research Abstract |
T cell were subjected to mechanical stress (MS) for periods ranging from 0-24h, via the use of a Flexell Strain Unit at 37℃ in a humidified 5% CO_2 atmosphere. After the stimulation, cells were collected for RNA extraction and condition culture medium was collected and stored until use in enzyme-linked immune sorbent assay (ELISA). Control samples were also collected in the same way. IL-17 secretion did not differ significantly between MS treated cells and control cells, but amount of IL-17 was slightly increased in MS cells. The effects of MS on IL-17A mRNA expressions were investigated by RT-PCR analysis. Mechanical stress with a Flexell Strain Unit is a useful form of mechanical stress to simulate T cell functioning under normal metabolism, but the amount of MS applied might not be suitable for detecting the expression of T cell cytokines, which was realized to have important functions in inflammatory responses.
The effects of MS on periodontal ligament (PDL) cells were indicated. Th
… More
e mRNA expressions of other cytokines and mediators related to bone remodeling during orthodontic treatment were investigated by RT-PCR analysis. IL-1β mRNA expression was showed steady state level on 0-4h, but was increased after 6hr and keep the expression until 24hr. MMP-1 mRNA expression was not changed under control condition and investigated on 2-24hr. IL-17RA mRNA increased the expression after addition of MS.
Intermittent tension force induced IL-1β mRNA expression but synthesis. This mechanical force also stimuli MMP-1 mRNA expression. MMP-1 mRNA expression was not induced by IL-1β, and it may be affected by intermittent mechanical force alone or other cytokines which were induced synthesis by mechanical forces. After the stimulation of IL-17, PDL cells were collected for RNA extraction and condition culture medium was collected and stored until use in enzyme-linked immune sorbent assay (ELISA). Control samples were also collected in the same way. MMP-1 and IL-1β secretion differ between IL-17 treated cells and controls. MMP-1 mRNA was increased after 24 hours and kept steady state level until 72hr. IL-1β mRNA was found to be increased after 24 hours and decreased at 72hr. This point will be investigated in detail. Less
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Research Products
(17 results)
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[Presentation] Effect of inorganic polyphosphate on osteoblastic and periodontal ligament cells
Author(s)
S., Uematsu, Y., Usui, H., NAKAYAMA, T., UEMATSU, K., FURUSAWA, S., KURIHARA, M., YAMAOKA
Organizer
85th General Session & Exhibition of the IADR
Description
「研究成果報告書概要(欧文)」より
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[Presentation] Replantation with or without cryopreservation of rat molars
Author(s)
S., KURIHARA, T, TANAKA, Y., USUI, Y., OSHIMA, S, UEMATSU, N., OKAFUJI
Organizer
85th General Session & Exhibition of the IADR
Description
「研究成果報告書概要(欧文)」より
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