2007 Fiscal Year Final Research Report Summary
Analysis of the relationship of periodontal susceptibility to periodontal dicease with bactericidal activity against P. gingivalis by periodontal epithelial cells
| Project/Area Number |
17390559
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Periodontal dentistry
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| Research Institution | Osaka University |
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Principal Investigator |
AMANO Atsuo Osaka University, Graduate School ofDentisty, Department of Oral Frontier Biology, Professor (50193024)
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| Co-Investigator(Kenkyū-buntansha) |
NAKAGAWA Ichiro The University of Tokyo, The Institute of Medical Science, Division of Bacteriology, Department of Infectious Disease Control, International Research Center for Infectious Diseases, Associate Professor (70294113)
INABA Hiroaki Osaka University, Graduate School ofDentisiry, Dept of Oral Frontier Biology, Assistant Professor (70359850)
KAWAI Shinji Osaka University, Graduate School ofDentisiry, Dept of Oral Frontier Biology, Assistant Professor (40362678)
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| Project Period (FY) |
2005 – 2007
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| Keywords | Dentistry / Bacteria / Infection / Cell・tissue / Signal transduction |
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| Research Abstract |
Porphyromonas gingivalis is a predominant periodontal pathogen that expresses a number of potential virulence factors involved in the pathogenesis of periodontitis. Gingival epithelial cells are spontaneously exposed to bacterial attacks and function to prevent invasion by bacteria into deeper tissues. P. gingivalis fimbriae are a critical factor for mediation of interaction of the organism with host tissues, as they promote both bacterial adhesion to and invasion of targeted sites. Fimbriae are capable of binding to human salivary components, extracellular matrix proteins, and commensal bacteria, while they also strongly adhere to cellular a581-integrin. Following adhesion to a581-integrin, P. gingivalis is captured by cellular pseudopodia, which enables invagination through an actin-mediated pathway. The invasive event has been reported to require host cellular dynamin, actin fibers, microtubules, and lipid rafts. Following passage through the epithelial barrier, the intracellular pathogen impairs cellular function. Fimbriae are classified into 6 genotypes (types I to V and Ib) based on the diversity of the fimA genes encoding each fimbria subunit, and intracellular P. gingivalis with type II fimbriae has been found to clearly degrade integrin-related signaling molecules, paxillin, and focal adhesion kinase, which disables cellular migration and proliferation. These events are considered to integrate the bacterial strategy for persistence in periodontal tissues.
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Research Products
(101 results)
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[Journal Article] Distribution of Porphyromonas gingivalis fimA genotypes in Japanese children and adolescents
Author(s)
Tamura, K, Nakano, K, Nomura, R, Miyake, S, Nakagawa, I, Amano, A, Ooshima, T
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Journal Title
Journal of Periodontology 76(5)
Pages: 674-679
Description
「研究成果報告書概要(欧文)」より
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[Journal Article] Impaired degradation of matrix collagen in human gingival fibroblasts by the anti-epileptic drug phenytoin
Author(s)
Kato, T, Okahashi, N, Kawai, S, Kato, T, Inaba, H, Morisaki, I, Amano, A
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Journal Title
Journal of Periodontology 76(6)
Pages: 941-950
Description
「研究成果報告書概要(欧文)」より
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[Journal Article] Apple- and hop-polyphenols protect periodontal ligament cells stimulated with enamel matrix derivative from Porphyromonas gingivalis
Author(s)
Inaba, H, Tagashira, M, Kanda, T, Ohno, T, Kawai, S, Amano, A
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Journal Title
Journal of Periodontology 76(12)
Pages: 2223-2229
Description
「研究成果報告書概要(欧文)」より
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[Journal Article] Molecular dissection of internalization of Porphyromonas gingivalis by cells using fluorescent beads coated with bacterial membrane vesicle
Author(s)
Tsuda, K, Amano, A, Umebayashi, K, Inaba, H, Nakagawa, I, Nakanishi, Y, Yoshimori, T
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Journal Title
Cell Structure and Function 30(2)
Pages: 81-91
Description
「研究成果報告書概要(欧文)」より
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[Journal Article] Relationship of periodontal bacteria and Porphyromonas gingivalis fimA variations with phenytoin-induced gingival overgrowth
Author(s)
Akiyama, S*, Amano, A, Kato, T, Takada, Y, Kimura, KR, Morisaki, I
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Journal Title
Oral Diseases 12(1)
Pages: 51-56
Description
「研究成果報告書概要(欧文)」より
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[Journal Article] Relationship of serum advanced glycation end products with deterioration of periodontitis in type 2 diabetes patients
Author(s)
Takeda, M, Ojima, M, Yoshioka, H, Inaba, H, Kogo, M, Shizukuishi, S, Nomura, M., Amano, A
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Journal Title
Journal of Periodontology 77(1)
Pages: 15-20
Description
「研究成果報告書概要(欧文)」より
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[Journal Article] Effect of phenytoin on collagen accumulation by human gingival fibroblasts exposed to TNF-a, in vitro
Author(s)
Kato, T, Okahashi, N, Ohno, T, Inaba, H, Kawai, S, Amano, A
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Journal Title
Oral Diseases 12(2)
Pages: 156-162
Description
「研究成果報告書概要(欧文)」より
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[Journal Article] Proinflammatory gene expression in mouse ST2 osteoblast/stromal cell line in response to infection by Porphyromonas gingivalis
Author(s)
Ohno, T, Okahashi, N*, Kawai, S, Kato, T, Inaba, H, Shibata, Y, Morisaki, I, Abiko, Y, Amano, A
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Journal Title
Microbes and Infection 8(4)
Pages: 1025-1034
Description
「研究成果報告書概要(欧文)」より
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[Journal Article] Inhibitory effects of tumor necrosis factor-a on migration of human periodontal ligament cells
Author(s)
Takemura, A, Nakagawa, I, Kawai, S, Inaba, H, Kato, T, Hamada, S, Amano, A
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Journal Title
Journal of Periodontology 77(5)
Pages: 883-890
Description
「研究成果報告書概要(欧文)」より
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[Journal Article] Invasion of epithelial cells and proteolysis of cellular focal adhesion components by distinct fimbria types of Porphyromonas gingivalis
Author(s)
Nakagawa, I, Inaba, H, Yamamura, T, Kato, T, Kawai, S, Ooshima, T, Amano, A
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Journal Title
Infection and Immunity 74(7)
Pages: 3773-3782
Description
「研究成果報告書概要(欧文)」より
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[Journal Article] Isolation and characterization of Streptococcus mutans in heart valve and dental plaque specimens from a patient with infective endocarditis
Author(s)
Nomura, R, Nakano, K, Nemoto, H, Fujita, K, Inagaki, S, Takahashi, T, Taniguchi, K, Takeda, M, Yoshioka, H, Amano, A, Ooshima, T
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Journal Title
Journal of Medical Microbiology 56(4)
Pages: 551-556
Description
「研究成果報告書概要(欧文)」より
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[Journal Article] Detection of cariogenic Streptococcus mutans in extirpated heart valve and atheromatous plaque specimens
Author(s)
Nakano, K, Inaba, H, Nomura, R, Nemoto, H, Takeda, M, Yoshioka, H, Matsue, H, Takahashi, T, Taniguchi, K, Amano, A, Ooshima, T
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Journal Title
Journal of Clinical Microbiology 44(9)
Pages: 3313-3317
Description
「研究成果報告書概要(欧文)」より
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[Journal Article] Distribution of genotypes of Porphyromonas gingivalis in type 2 diabetic patients with periodontitis in Mexico
Author(s)
Davila-Perez, C, Amano, A, Alpuche-Solis, AG, Patino-Marin, N, Pontigo-Loyola, AP, Hamada, S, Loyola-Rodriguez, JP
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Journal Title
Journal of Clinical Periodontology 34(1)
Pages: 25-30
Description
「研究成果報告書概要(欧文)」より
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[Journal Article] Virulence of Porphyromonas gingivalis is altered by substitution of fimbria gene with different genotype
Author(s)
Kato, T, Kawai, S, Nakano, K, Inaba, H, Kuboniwa, M, Nakagawa, I, Tsuda, K, Omori, H, Ooshima, T, Yoshimori, T, Amano, A
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Journal Title
Cellular Microbiology 9(3)
Pages: 753-765
Description
「研究成果報告書概要(欧文)」より
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[Journal Article] Detection and serotype distribution of Actinobacillus actinomycetemcomitans in cardiovascular specimens from Japanese patients
Author(s)
Nakano, K, Inaba, H, Nomura, R, Nemoto, H, Tamura, K, Miyamoto, E, Yoshioka, H, Taniguchi, K, Amano, A, Ooshima, T
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Journal Title
Oral Microbiology and Immunology 22(2)
Pages: 136-139
Description
「研究成果報告書概要(欧文)」より
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[Journal Article] Serotype distribution of Streptococcus mutans a pathogen of dental caries in cardiovascular specimens from Japanese patients
Author(s)
Nakano, K, Nemoto, H, Nomura, R, Homma, H, Yoshioka, H, Shudo, Y, Hata, H, Toda, K, Taniguchi, K, Amano, A, Ooshima, T
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Journal Title
Journal of Medical Microbiology 56(4)
Pages: 551-556
Description
「研究成果報告書概要(欧文)」より
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[Journal Article] Maturation of fimbria precursor protein by exogenous gingipains in Porphyromonas gingivalis gingipain-null mutant
Author(s)
Kato, T, Tsuda, T, Omori, H, Kato, T, Yoshimori, T, Amano, A
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Journal Title
FEMS Microbiology Letters 273
Pages: 96-102
Description
「研究成果報告書概要(欧文)」より
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[Journal Article] Detection of novel serotype k Streptococcus mutans in infective endocarditis patients
Author(s)
Nakano, K, Nomura, R, Nemoto, H, Mukai, T, Yoshioka, H, Shudo, Y, Hata, H, Toda, K, Taniguchi, K, Amano, A, Ooshima
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Journal Title
Journal of Medical Microbiology 56(10)
Pages: 1413-1415
Description
「研究成果報告書概要(欧文)」より
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[Journal Article] Inflammatory responses of gingival epithelial cells stimulated with Porphyromonas gingivalis vesicles are inhibited by hop-associated polyphenols
Author(s)
Kou, Y, Inaba, H, Kato, T, Tagashira, M, Honma, D, Kanda, T, Ohtake, Y, Amano, A
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Journal Title
Journal of Periodontology 79(1)
Pages: 174-180
Description
「研究成果報告書概要(欧文)」より
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[Journal Article] Heterogenic virulence and related factors among clinical isolates of Porphyromonas gingivalis with type II fimbriae
Author(s)
Inaba, H, Nakano, K, Kato, T, Nomura, R, Kawai, S, Kuboniwa, M, Ishihara, K, Ooshima, T, Amano, A
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Journal Title
Oral Microbiology and Immunology 23(1)
Pages: 29-35
Description
「研究成果報告書概要(欧文)」より
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[Journal Article] Porphyromonas gingivalis gingipains cause G1 arrest in osteoblastic/stromal cells
Author(s)
Kato, T, Tsuda, T, Kawai, S, Inaba, H, Okahashi, N, Shibata, Y, Abiko, Y, Amano, A
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Journal Title
Oral Microbiology and Immunology 23(2)
Pages: 158-164
Description
「研究成果報告書概要(欧文)」より
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[Journal Article] Distribution of Porphyromonas gingivalis fimA genotypes in cardiovascular specimens from Japanese patients
Author(s)
Nakano, K, Inaba, H, Nomura, R, Nemoto, H, Takeuchi, H, Yoshioka, H, Toda, K, Taniguchi, K, Amano, A, Ooshima, T
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Journal Title
Oral Microbiology and Immunology 23(2)
Pages: 170-172
Description
「研究成果報告書概要(欧文)」より
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[Journal Article] Cellular motility of Down syndrome gingival fibroblasts susceptible to impairment by Porphyromonas gingivalis invasion
Author(s)
Murakami, J, Kato, T, Kawai, S, Akiyama, S, Amano, A, Morisaki, I
Description
「研究成果報告書概要(欧文)」より
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[Journal Article] P. gingivalis accelerates gingival epithelial cell progression through the cell cycle
Author(s)
Kuboniwa, M, Hasegawa, Y, Mao, S, Shizukuishi, S, Amano, A, Lamont, RJ*, Yilmaz, O
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Journal Title
Microbes and Infection (in press) (Epub ahead of print)
Description
「研究成果報告書概要(欧文)」より
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[Journal Article] Relationship of Porphyromonas gingivalis with glycemic level in patients with type 2 diabetes following periodontal treatment
Author(s)
Makiura, N, Ojima, M, Kou, Y, Furuta, N, Okahashi, N, Shizukuishi, S, Amano, A
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Journal Title
Oral Microbiology and Immunology 23(4)(in press)
Description
「研究成果報告書概要(欧文)」より
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[Journal Article] Identification of hop polyphenolic components which inhibit prostaglandin E_2 production by gingival epithelial cells stimulated with periodontal pathogen
Author(s)
Inaba, H, Tagashira, M, Honma, D, Kanda, T, Kou, Y, Ohtake, Y, Amano, A
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Journal Title
Biological & Pharmaceutical Bulletin 31(3)
Pages: 527-530
Description
「研究成果報告書概要(欧文)」より
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[Journal Article] Functional analysis of a5β1 integrin and lipid rafts in invasion of epithelial cells by Porphyromonas gingivlis using fluorescent beads coated with bacterial membrane vesicle
Author(s)
Tsuda, K, Furuta, N, Inaba, H, Kawai, S, Umebayashi, K, Hanada, K, Yoshimori, T, Amano, A
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Journal Title
Cell Structure and Function,Apr 4(Epub ahead of print)
Description
「研究成果報告書概要(欧文)」より
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[Journal Article] Multiple dental caries in unerupted permanent teeth in a child with phenytoin-induced gingival overgrowth
Author(s)
Murakami, J*, Morisaki, I, Tanaka, T, Akiyama, S, Amano, A, Friedman, SC
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Journal Title
Journal of Disability and Oral Health 9(1)
Pages: 13-16
Description
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[Presentation] Effect of genotypes of Porphyromonas gingivalis fimbriae on biofilm formation2007
Author(s)
Kuboniwa, M, Amano, A, Kato, T, Iwasaki, M, Hashino, E, Shizukuishi, S
Organizer
55th Annual Meeting of Japanese Association for Dental Research
Place of Presentation
Yokohama
Year and Date
2007-11-17
Description
「研究成果報告書概要(欧文)」より
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[Presentation] Porphyromonas gingivalis accelerates cell cycle progression in gingival epithelial cells2007
Author(s)
Kuboniwa, M, Hasegawa, Y, Mao, S, Amano, A, Shizukuishi, S, Lamont, R
Organizer
85th General session of the IADR
Place of Presentation
New Orleans, USA
Year and Date
2007-03-23
Description
「研究成果報告書概要(欧文)」より
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[Presentation] Cellular impairment by distinct fimbria types of P. gingivalis2006
Author(s)
Amano, A, Kato, T, Inaba, H, Kawai, S, Nakagawa, I
Organizer
84th General session of the IADR
Place of Presentation
Brisbane, Australia
Year and Date
2006-06-28
Description
「研究成果報告書概要(欧文)」より
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[Book] 歯科医学の最先端2008
Author(s)
天野敦雄, 他4名(分担)
Total Pages
321
Publisher
大阪大学出版会
Description
「研究成果報告書概要(和文)」より
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