2006 Fiscal Year Final Research Report Summary
Study on Design of Biodegradable Nanoparticles with Function of Controlled Drug Release
| Project/Area Number |
17500308
|
|---|
| Research Category |
Grant-in-Aid for Scientific Research (C)
|
| Allocation Type | Single-year Grants |
|---|
| Section | 一般 |
|---|
| Research Field |
Biomedical engineering/Biological material science
|
|---|
| Research Institution | Kyushu Institute of Technology |
|---|
Principal Investigator |
OKAMOTO Kouji Kyushu Institute of Technology, Faculty of Computer Science and Systems Engineering, Professor, 情報工学部, 教授 (40122618)
|
|---|
| Co-Investigator(Kenkyū-buntansha) |
KAIBARA Kozue Kyushu Kyoritsu University, Faculty of Engineering, Professor, 工学部, 教授 (90080564)
MAEDA Iori Kyushu Institute of Technology, Faculty of Computer Science and Systems Engineering, Research Associate, 情報工学部, 助手 (50311858)
|
|---|
| Project Period (FY) |
2005 – 2006
|
| Keywords | Controlled drug release / Coacervate droplet / γ-irradiation / nanoparticles / Biodegradability / Phosphorylation-response / Redox-response |
|---|
| Research Abstract |
The high polymer of repeating pentapeptide sequence, (VPGVG)n simply Vpp, is basis for phosphorylation-responsible and redox-responsible controlled release devices. Vpp undergoes self-assembly called coacervation, where coacervate droplets with diameter in the range of 400 to 600 nm are formed. The nanoparticles cross-linked by cobalt-60 γ-irradiation of coacervate droplets are useful as drug release devices. Vpp and random copolymers, [8(VPGVG), 2(VPGK(Z)G)] simply VK(Z)pp and [8(VPGVG), 2(VPGK(Boc)G)] simply VK(Boc)pp, where the molar ratio of VPGVG to VPGK(Z)G or VPG(Boc)G is 8 to 2 were synthesized. The coacervate droplets of these polymer and copolymers in the coacervate state at 55-60'C were cross-linked by cobalt-60 γ-irradiation to yield nanoparticles in the range of 200 to 400 nm. The nanoparticles obtained were stable in the treatment of enzyme. The loading antitumor drug, adriamycin, into Vpp-derived and VK(Boc)pp-derived nanoparticles were 80 and 77%, respectively. The release of adriamycin from Vpp-derived and VK(Boc)pp-derived nanoparticles were 13 and 19 days (time for releasing half amount of loaded drug), respectively. Next we synthesized two random copolymers, [30(VPGVG), (RGYSL)] and [7(V PGV G), 3 (VPGK(NMeN)G)], where RGYSL which is a peptide substrate for phosphorylation by protein kinase and VPGK(NMeN)G which is a redox peptide with N-methylnicotinamide were added. The onset temperature of self-assembly of [30(VPGVG), (RGYSL)] shifted to higher temperature by phosphorylation and to lower temperature by dephosphorylation. Moreover, the onset temperature of self-assembly of [7(VPGVG), 3(VPGK(NMeN)G)] shifted to higher temperature by oxidation and to lower temperature by reduction. Further study on controlled drug release from nanoparticles of [30(VPGVG), (RGYSL)] and [7(VPGVG), 3(VPGK(NMeN)G)] is underway.
|
Research Products
(12 results)
-
-
-
-
-
[Journal Article] Domains 16 and 17 of tropoelastin in elastin fibre formation2007
Author(s)
H.Wachi, F.Sato, J.Nakazawa, R.Nonaka, Z.Szabo, Z.Urban, T.Yasunaga, I.Maeda, K.Okamoto, B.C.Stacher, D.Y.Li, R.P.Mecham, Y.seyama
-
Journal Title
Biochem. J. 402
Pages: 63-70
Description
「研究成果報告書概要(欧文)」より
-
-
-
-
-
-
-
