Regeneration of the Pancreatic Polypeptide Cells in Experimental and Clinical Study

2007 Fiscal Year Final Research Report Summary

• Project/Area Number: 17590155
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: General anatomy (including Histology/Embryology)
• Research Institution: Tokyo Medical University
• Principal Investigator: YI Shuang-Qin Tokyo Medical University, School of Medicine, Assistant Professor (70334753)
• Co-Investigator(Kenkyū-buntansha): MIYAKI Takayoshi Tokyo Medial University, School of Medicine, Associate Professor (60366094) ; ITO Masahiro Tokyo Medial University, School of Medicine, Professor (00232471) ; TERAYAMA Hayato Tokyo Medial University, School of Medicine, Instructor (00384983)
• Project Period (FY): 2005 – 2007
• Keywords: Ventral Pancreas / Pancreatic Polypeptide / Regeneration / Obesity / Appetite / Food Intake / Neuropeptide Y / Peptide YY

Research Abstract

In our previous study, it indicated that the pancreatic polypeptide-immunoreactive cells (PP cells) were extremely abundant in the right lobe of the pancreas in the house musk shrew (Suncus murinus) similar to that of the PP cells in the uncinate process of human pancreas. It indicated that the right and left lobes of the Suncus pancreas corresponded to the ventral and dorsal pancreas. In this study, the ventral pancreas was excised while the dorsal pancreas was preserved in adult Suncus. After 1, 2, 3 weeks or 1, 2, 3 months of the operation, the animals were injected ip with BrdU before sacrificed. The dorsal pancreas, duodenum and stomach were collected for immunocytochemical and Western blotting analysis with insulin, glucagon, somatostatin, PP and BrdU. After 3-4 weeks of the resection of the ventral pancreas, the PP-positive cells appeared, redistributed within the Langerhans islets of the dorsal pancreas remained.
In addition, employing the specimen of the pancreas, after pancreaticoduodenectomy (PD), the PP positive cells were observed to redistribute in the remaining pancreas which correspond to the dorsal pancreas. Moreover, it was confirmed that the amount of the PP positive reaction cell was a positive correlation with time the passing time of after the operation.
The results implied that the PP-positive cells could be regenerated after these cells damaged or lost, and the converted cells exist in the dorsal pancreas.

Research Products

• [Journal Article] Splenic cytokines in mice immunized with testicular germ cells (2008)
  • Author(s): Tokunaga Y, Terayama H, Naito M, Qu N, Hirai S, Ogawa Y, Yi SQ, Itoh M.
  • Journal Title: Int J Androl.
  • Description: 「研究成果報告書概要(和文)」より
  • Peer Reviewed
• [Journal Article] A rare case of inferior mesenteric artery arising from the superior mesenteric artery,with a review of the review of the literature (2008)
  • Author(s): Yi SQ, Li J, Terayama H, et. al.
  • Journal Title: Surgical and Radiologic Anatomy 30 Pages: 159-165
  • Description: 「研究成果報告書概要(和文)」より
  • Peer Reviewed
• [Journal Article] Absence of the celiac trunk:case report and review of the literature (2008)
  • Author(s): Yi SQ, Terayama H, Naito M, Hirai S, Alimujang S, Yi N, Tanaka S, Itoh M.
  • Journal Title: Clin Anat 21 Pages: 283-286
  • Description: 「研究成果報告書概要(和文)」より
  • Peer Reviewed
• [Journal Article] A study of the accessory hepatic vein to segments VI and VII with a morphological reconsideration of the human liver (2008)
  • Author(s): Buhe S, Miyaki T, Saito T, Sawuti A, Terayama H, Naito M, Yi SQ, Itoh M.
  • Journal Title: Surg Radiol Anat 30 Pages: 201-207
  • Description: 「研究成果報告書概要(和文)」より
  • Peer Reviewed
• [Journal Article] Structure of Thymic Cysts in Congenital Lymph Nodes-lacking Mice (2008)
  • Author(s): Nakamura Y, Qu N, Terayama H, Naito M, Yi SQ, Moriyama H, Itoh M.
  • Journal Title: Anat Histol Embryol 37 Pages: 126-130
  • Description: 「研究成果報告書概要(和文)」より
  • Peer Reviewed
• [Journal Article] Absence of the celiac trunk : case report and review of the literature (2008)
  • Author(s): Yi, SO, Teravama, H, Naito, M, Hirai, S, Alimujang, S, Yi, N, Tanaka, S, Itoh, M
  • Journal Title: Clinical Anatomy (in press)
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] A study of the accessory hepatic vein to segments VI and VII with a morphological reconsideration of the human liver (2008)
  • Author(s): Buhe, S, Miyaki, T, Saito, T, Sawuti, A, Terayama, H, Naito, M, Yi, SQ, Itoh, M
  • Journal Title: Surgical and Radiologic Anatomy
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] A rare case of inferior mesenteric artery arising from the superior mesenteric artery, with a review of the review of the literature (2008)
  • Author(s): Yi, SQ, Li, J, Teravama, H, Naito, M, Iimura, A, Itoh, M
  • Journal Title: Surgical and Radiologic Anatomy 30(2) Pages: 159-165
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Structure of Thymic Cysts in Congenital Lymph Nodes-lacking Mice (2008)
  • Author(s): Nakamura, Y, Qu, N, Terayama, H, Naito, M, Yi, SQ, Moriyama, H, Itoh, M
  • Journal Title: Anatomia Histologia Embryologia 37(2) Pages: 126-130
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Gross anatomical study of the sympathetic cardiac nerves in the house musk shrew(Suncus murinus) (2007)
  • Author(s): Tanaka A, Tanaka S, Yi SQ, et. al.
  • Journal Title: The Anatomical Record 290 Pages: 468-476
  • Description: 「研究成果報告書概要(和文)」より
  • Peer Reviewed
• [Journal Article] Sequential Histopathology of Pancreatic Tissues in aly/aly Mice (2007)
  • Author(s): Nakamura Y, Yi SQ, Terayama H, et. al.
  • Journal Title: Cells Tissues Organs 186 Pages: 204-209
  • Description: 「研究成果報告書概要(和文)」より
  • Peer Reviewed
• [Journal Article] A common celiacomesenteric trunk,and a brief review of the literature (2007)
  • Author(s): Yi SQ, Terayama H, Naito M, Hayashi S, Moriyama H, Tsuchida A, Itoh M.
  • Journal Title: Ann Anat. 189 Pages: 482-488
  • Description: 「研究成果報告書概要(和文)」より
  • Peer Reviewed
• [Journal Article] The quail and chicken intestine have sialyl-Gal sugar chains responsible for the binding of influenza A viruses to human type receptors (2007)
  • Author(s): Guo CT, Takahashi N, Yagi H, Kato K, Takahashi T, Yi SQ, Chen Y, Ito T, Otsuki K, Kida H, Kawaoka Y, Hidari KI, Miyamoto D, Suzuki T, Suzuki Y.
  • Journal Title: Glycobiology 17 Pages: 713-724
  • Description: 「研究成果報告書概要(和文)」より
  • Peer Reviewed
• [Journal Article] Surgical anatomy of innervation of the gallbladder in humans and Suncus murinus with special reference to morphological understanding of gallstone formation after gastrectomy (2007)
  • Author(s): Yi SQ, Ohta T, Tsuchida A, Terayama H, Naito M, Li J, Wang HX, Yi N, Tanaka S, Itoh M.
  • Journal Title: World J Gastroenterol 13 Pages: 2066-2071
  • Description: 「研究成果報告書概要(和文)」より
  • Peer Reviewed
• [Journal Article] Short term effects of di-(2-ethylhexyl) phthalate on testes,liver,kidneys and pancreas in mice (2007)
  • Author(s): Miura Y, Naito M, Ablake M, Terayama H, Yi SQ, Qu N, Cheng LX, Suna S, Jitsunari F, Itoh M.
  • Journal Title: Asian J Androl 9 Pages: 199-205
  • Description: 「研究成果報告書概要(和文)」より
  • Peer Reviewed
• [Journal Article] Splenic cytokines in mice immunized with testicular germ cells (2007)
  • Author(s): Tokunaga, Y, Teravama, H, Naito, M, Qu, N, Hirai, S, Ogawa, Y, Yi, SO, Itoh, M
  • Journal Title: International Journal of Andrology
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] A common celiacomesenteric trunk, and a brief review of the literature (2007)
  • Author(s): Yi, SO, Teravama, H, Naito, M, Hayashi, S, Moriyama, H, Tsuchida, A, Itoh, M
  • Journal Title: Annals of Anatomy 189(5) Pages: 482-488
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Sequential Histopathology of Pancreatic Tissues in aly/aly Mice (2007)
  • Author(s): Nakamura, Y, Yi, SQ, Terayama, H, Naito, M, Li, J, Moriyama, H, Tsuchida, A, Itoh, M
  • Journal Title: Cells Tissues Organs 186(3) Pages: 204-209
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] The quail and chicken intestine have sialyl-Gal sugar chains responsible for the binding of influenza A viruses to human type receptors (2007)
  • Author(s): Guo, CT, Takahashi, N, Yagi, H, Kato, K, Takahashi, T, Yi, SO, Chen, Y, Ito, T, Otsuki, K, Kida, H, Kawaoka, Y, Hidari, KI, Miyamoto, D, Suzuki, T, Suzuki, Y
  • Journal Title: Glycobiology 17(7) Pages: 713-724
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Gross anatomical study of the sympathetic cardiac nerves in the house musk shrew(Suncus murinus) (2007)
  • Author(s): Tanaka, A, Tanaka, S, Miyamoto, K, Yi, SQ, Nakatani, T
  • Journal Title: The Anatomical Record 290(5) Pages: 468-476
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Surgical anatomy of innervation of the gallbladder in humans and Suncus murinus with special reference to morphological understanding of gallstone formation after gastrectomy (2007)
  • Author(s): Yi, SQ, Ohta, T, Tsuchida, A, Terayama, H, Naito, M, Li, J, Wang, HX, Yi, N, Tanaka, S, Itoh, M
  • Journal Title: World Journal of Gastroenterology 13(14) Pages: 2066-2071
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Short term effects of di-(2-ethylhexyl) phthalate on testes, liver, kidneys and pancreas in mice (2007)
  • Author(s): Miura, Y, Naito, M, Ablake, M, Terayama, H, Yi, SO, Qu, N, Cheng, LX, Suna, S, Jitsunari, F, Itoh, M
  • Journal Title: Asian Journal of Andrology 9(2) Pages: 199-205
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Complete ureteral duplication associated with megaureter and ureteropelvic junction dilatation:report on an adult cadaver case with a brief review of the literature (2006)
  • Author(s): Iimura A, Yi SQ, Terayama H, Naito M, Buhe S, Oguchi T, Takahashi T, Miyaki T, Itoh M.
  • Journal Title: Ann Anat 188 Pages: 371-375
  • Description: 「研究成果報告書概要(和文)」より
  • Peer Reviewed
• [Journal Article] A case of spontaneous splenorenal shunt associated with splenic artery aneurysm (2006)
  • Author(s): Hayashi S, Yi SQ, Miyaki T, Nakamura Y, Terayama H, Naito M, Itoh M.
  • Journal Title: Surg Radiol Anat 28 Pages: 311-315
  • Description: 「研究成果報告書概要(和文)」より
  • Peer Reviewed
• [Journal Article] Surgical anatomy of the innervation of pylorus in human and Suncus murinus,in relation to surgical technique for pylorus-preserving pancreaticoduodenectomy (2006)
  • Author(s): Yi SQ, Ru F, Ohta T, Terayama H, Naito M, Hayashi S, Buhe S, Yi N, Miyaki T, Tanaka S, Itoh M.
  • Journal Title: World J Gastroenterol 12 Pages: 2209-2216
  • Description: 「研究成果報告書概要(和文)」より
  • Peer Reviewed
• [Journal Article] Complete ureteral duplication associated with megaureter and ureteropelvic junction dilatation : report on an adult cadaver case with a brief review of the literature (2006)
  • Author(s): Iimura, A, Yi, SQ, Teravama, H, Naito, M, Buhe, S, Oguchi, T, Takahashi, T, Miyaki, T, Itoh, M
  • Journal Title: Annals of Anatomy 188(4) Pages: 371-375
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] A case of spontaneous splenorenal shunt associated with splenic artery aneurysm (2006)
  • Author(s): Hayashi, S, Yi, SQ, Miyaki, T, Nakamura, Y, Teravama, H, Naito, M, Itoh, M
  • Journal Title: Sugical and Radiologic Anatomy 28(3) Pages: 311-315
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Surgical anatomy of the innervation of pylorus in human and Suncus murinus, in relation to surgical technique for pylorus-preserving pancreaticoduodenectomy (2006)
  • Author(s): Yi, SO, Ru, F, Ohta, T, Terayama, H, Naito, M, Hayashi, S, Buhe, S, Yi, N, Miyaki, T, Tanaka, S, Itoh, M
  • Journal Title: World Journal of Gastroenterology 12(14) Pages: 2209-2216
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Surgical anatomy of the innervation of the major duodenal papilla in human and Suncus murinus,from the perspective of preserving innervation in organ-saving procedures (2005)
  • Author(s): Yi SQ, Ohta T, Miwa K, Shimokawa T, Akita K, Itoh M, Miyamoto K, Tanaka S.
  • Journal Title: Pancreas 30 Pages: 211-217
  • Description: 「研究成果報告書概要(和文)」より
  • Peer Reviewed
• [Journal Article] Surgical anatomy of the innervation of the major duodenal papilla in human and Suncus murinus, from the perspective of preserving innervation in organ-saving procedures (2005)
  • Author(s): Yi, SQ, Ohta, T, Miwa, K, Shimokawa, T, Akita, K, Itoh, M, Miyamoto, K, Tanaka, S
  • Journal Title: Pancreas 30(3) Pages: 211-217
  • Description: 「研究成果報告書概要(欧文)」より
• [Presentation] スンクスの膵臓と消化管におけるNPYファミリとレセプターの免疫局在 (2007)
  • Author(s): 李 軍, 易 勤, 斯欽 布和, ほか
  • Organizer: 日本解剖学会 第112回総会
  • Place of Presentation: 大阪
  • Year and Date: 20070327-29
  • Description: 「研究成果報告書概要(和文)」より
• [Presentation] スンクスにおける膵炎、膵線維化モデル研究 (2007)
  • Author(s): 王 恒孝, 易 勤, 李 軍, 寺山 隼人, 内藤 宗和, 林 省吾, 伊藤 正裕
  • Organizer: 日本解剖学会 第112回総会
  • Place of Presentation: 大阪
  • Year and Date: 20070327-29
  • Description: 「研究成果報告書概要(和文)」より
• [Presentation] 腹腔動脈幹の分岐異常の3例報告 (2007)
  • Author(s): 易 勤, 寺山 隼人, 内藤 宗和, 林 省吾, 伊藤 正裕
  • Organizer: 日本解剖学会 第112回総会
  • Place of Presentation: 大阪
  • Year and Date: 20070327-29
  • Description: 「研究成果報告書概要(和文)」より
• [Presentation] Immunolocalization of NPY Family and its Receptors in the Pancreas and the Gastrointestinal Tract of the House Musk Shrew, Suncus Murinus (2007)
  • Author(s): Jun, Li, Shuang-Qin, Yi, et. al.
  • Organizer: 112th convention of Japanese Association of Anatomisis
  • Place of Presentation: Osaka
  • Year and Date: 20070327-29
  • Description: 「研究成果報告書概要(欧文)」より
• [Presentation] Study of Experimental model of Pancreatitis and Fiberosis of Pancreas in the House Musk Shrew, Suncus Murinus (2007)
  • Author(s): Heng-Xiao, Wang, Shuang-Qin, Yi, et. al.
  • Organizer: 112th convention of Japanese Association of Anatomisis
  • Place of Presentation: Osaka
  • Year and Date: 20070327-29
  • Description: 「研究成果報告書概要(欧文)」より
• [Presentation] Variation of the celiac trunk : three cases report (2007)
  • Author(s): Shuang-Qin, Yi, et. al.
  • Organizer: 112th convention of Japanese Association of Anatomisis
  • Place of Presentation: Osaka
  • Year and Date: 20070327-29
  • Description: 「研究成果報告書概要(欧文)」より