2007 Fiscal Year Final Research Report Summary
Structure and function of leuoocyte chemotactic lectin in parasitic infection
| Project/Area Number |
17590371
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Parasitology (including Sanitary zoology)
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| Research Institution | The University of Tokushima |
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Principal Investigator |
OHASHI Makoto The University of Tokushima, Faculty of Integrated Arts and Sciences, Professor (40128369)
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| Project Period (FY) |
2005 – 2007
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| Keywords | malaria / neutrophil / lectin / ubiquitin / Schistosoma / chitinase / Anopheles / eosinophil |
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| Research Abstract |
In Plasmodium infection, dramatic increase of neutrophil chemotactic activity was observed in the lysate of red blood cells km peripheral blood. The neutrophil chemotactic factor was purified by a combination of chromatographies, and the N-terminus aminoacid sequence of the protein was found to be identical with an ubiquitin family protein (IP18). The time course of the expression of IP18 was examined in each organ in Plasmodium infection. When the epitope of neutrophil chemotactic activity of IP18 was analyzed, it is clearly shown to be independent of fMLP (formyl-Met-Leu-Phe), TfiNCF (Tritrichomonas foetus), DiNCF (Dirofilaria immitis), GRO (chemokine). Analysis of the neutrophil chemotactic activity of various truncate forms of recombinant IP18 revealed that aminoacid sequence 78-88 was necessary for the expression of neutrophil chemotactic activity. IP18 showed hemaggutination activity, and C terminus 143-160 played important role for the hemaggutination activity. We also showed an eosinophil chemotactic activity in saliva or midgut extract of anopheline mosquitoes that transmit malaria parasites. This eosinophil chemotactic factor was identified as a chitinase family protein akin to eosinophil chemotactic factor produced in schistosomiasis. Antibody to chitinase was observed in the majority of residents sera in malaria endemic area, Solomon islands. The titer of aanti-chitinase was not correlated with the parasitemia of P. farciparum parasites. Thus, leukocyte chemotactic lectin would possess significant role for parasitic infections.
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Research Products
(15 results)
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[Presentation] 虫卵結節形成と好中球2005
Author(s)
大橋 眞
Organizer
第61回日本寄生虫学会西日本支部大会
Place of Presentation
高知市
Year and Date
2005-11-04
Description
「研究成果報告書概要(和文)」より
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