2006 Fiscal Year Final Research Report Summary
Re-evaluation of charge selective protein sieving barrier in nephrotic syndrome
| Project/Area Number |
17591118
|
|---|
| Research Category |
Grant-in-Aid for Scientific Research (C)
|
| Allocation Type | Single-year Grants |
|---|
| Section | 一般 |
|---|
| Research Field |
Pediatrics
|
|---|
| Research Institution | Nihon University |
|---|
Principal Investigator |
TAKAHASHI Shori Nihon University, School of Medicine, Lecturer (60328755)
|
|---|
| Co-Investigator(Kenkyū-buntansha) |
MURAKAMI Hitohiko Nihon University, School of Medicine, Research Assistant (90386037)
NAGATA Michio University of Tsukuba, Institute of Basic Medical Science, Professor (10192238)
|
|---|
| Project Period (FY) |
2005 – 2006
|
| Keywords | charge barrier / charge selective barrier / proteinuria / nephrotic syndrome / IgA nephropathy / Dent disease / Charge selectivity / Charge selectivity index |
|---|
| Research Abstract |
RESEARCH RESULTS
In this study we noted that IgA and IgG have similar molecular size and distinct molecular pI, hence both were regarded as appropriate molecules for use in analyzing a glomerular capillary wall (GCW) charge selective function by differential clearance. We defined IgA/IgG clearance in terms of a charge selectivity index (CSI) for at least a size of these molecules. If the CSI is low, charge selective protein sieving property may function, although it may not function if the former approaches 1.0. We studied CSI in various glomerular disease and the results indicated that CSI showed around 1.0 in glomerulonephritis, which indicated that charge selective barrier does not function in glomerulonephritis such as IgA nephropathy, Henoch-Sh〓nlein purpura nephritis, membranoproliferative glomerulonephritis and Alport syndrome. In contrast, CSI in podocyte diseases showed around 0.4. This indicated that charge barrier in podocyte diseases such as steroid sensitive nephrotic syndrome, focal and segmental glomerulosclerosis and Finnish type congenital nephrotic syndrome functions to some degree. Then we studied CSI in Dent disease, because, the urine of Dent disease is a concentrate of glomerular filtrate without passing a process of proximal tubular protein reabsorption. This in turn means that the urine of Dent disease keeps a protein profile of normal glomerular filtrate. The preliminary results of CSI in Dent disease was around 0.2 indicating a existence of strong charge selective barrier in normal GCW. This also indicated that the charge selectivity of GCW in podocyte disease were less strong than normal subjects.
Besides of these works we studied a relationship between mental stress and relapse of nephrotic syndrome in frequently relapsing nephrotic syndrome. In addition, we studied the role of multiple drug resistance gene 1 in steroid sensitive nephrotic syndrome either.
|
Research Products
(8 results)
