2006 Fiscal Year Final Research Report Summary
The research about the application of RNAi phenomenon to radiation therapy : using hSMG1-siRNA
| Project/Area Number |
17591284
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Radiation science
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| Research Institution | Yokohama City University |
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Principal Investigator |
NAKAGAMI Yoshihiro Yokohama City University, Hospital, assistant (80347301)
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| Co-Investigator(Kenkyū-buntansha) |
OMURA Motoko Yokohama City University, Hospital, assistant (70244506)
OGINO Ichiro Yokohama City University, Hospital, assistant professor (20275035)
INOUE Tomio Yokohama City University, School of Medicine, professor (80134295)
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| Project Period (FY) |
2005 – 2006
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| Keywords | radiation sensitivity / siRNA / gene knock down |
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| Research Abstract |
It is the purpose of our research that we transfect siRNAs of genes about radiation sensitivity to various radiation-resistant tumor, change their radiation sensitivity, enhance radiation therapy effect and utilize this data for future gene-radiation therapy.
We transfected siRNAs planed to knock down genes about radiation sensitivity (ATM, hSMG1 et.) to mice with radiation-resistant tumor, and checked the change of quantity of their gene product, the change of growth curve and the change of their radiation sensitivity We made mice with radiation-resistant tumor by transplanting hepatitis cell carcinoma, and made mice with radiation-sensitive tumor by transplanting malignant lymphoma. We considered stickiness and width of tumor and decided the radiation dose which made a proper apoptosis without necrosis on the mice. We irradiated gamma ray to each mice. We checked the occurrence of apoptosis on each mice before and after the irradiation with TUNNEL method, and checked the change of quantity of ATM and hSMG1 gene products with real-time PCR method. ATM and hSMG1 gene products correlated with the occurrence of apoptosis.
On the other hand, we transfected siRNAs planed to knock down ATM or hSMG1 genes into tumors using in vivo siRNA transfection regent (TransIT In Vivo Polymer Solution), and checked the occurrence of apoptosis before and after the transfection and the irradiation with TUNNEL method, and checked the change of quantity of ATM and hSMG1 gene products with real-time PCR method. In the groups knocked down ATM and hSMG1 gene, the occurrence of apoptosis decreased.
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