2018 Fiscal Year Annual Research Report
Dissection of SINEUP ncRNAs, which have a potential to become next hot molecular biotechnology tool.
|Research Institution||Institute of Physical and Chemical Research |
Carninci Piero 国立研究開発法人理化学研究所, 生命医科学研究センター, 副センター長 (10333296)
|Foreign Research Fellow
RAHMAN MOHAMMED 国立研究開発法人理化学研究所, 生命医科学研究センター, 外国人特別研究員
|Project Period (FY)
2017-11-10 – 2020-03-31
|Keywords||SINEUP / EEA elements / SINEB2 elements|
|Outline of Annual Research Achievements
Project 1 - Dissecting physiological role of lncRNAs with SINEB2 elements: We have finalized the list of SINB2 containing lncRNAs (research plan; project2; a) that we want to dissect in details by analyzing different database analysis. We looked for their expression in different tissues and established cell lines and their involvement in different patho-/physiological functions. Moreover, we started with and got some initial data regarding research plan; project2; a) & b).
Project 2 - SINEUP Sequence motifs and RNA binding proteins (RBPs): We finalized the protocol for ‘modified eCLIP’ in house. We also prepared the initial libraries for sequencing.
|Current Status of Research Progress
Current Status of Research Progress
2: Research has progressed on the whole more than it was originally planned.
We added a new Aim-2 in order to address our original research question to dissect the mechanism of SINEUPs. So far, the research activity to address the new aim moving forward smoothly. I strongly believe, we can finish the projects on time.
|Strategy for Future Research Activity
Project 1: Dissecting physiological role of lncRNAs with SINEB2 elements
1) Defining relationship between IncRNA/SINEB2 and sense protein coding genes by knockdown and overexpression experiments, 2) Optimizing protocols (RNA FISH, ChRIP-Seq/MS etc) to evaluate the mechanism of action of IncRNA/SINEB2.
Project 2: SINEUP Sequence motifs and RNA binding proteins (RBPs)
1) Finalize modified eCLIP protocol in house, 2) Generate biological replicate eCLIP library for validation, 3) Sequencing and data analysis, 4) Write manuscript and present our finding at international conferences.