Unravelling fundamental interactions underlying the rotation mechanism of F1-ATPase

2019 Fiscal Year Final Research Report

• Project/Area Number: 17K05562
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Mathematical physics/Fundamental condensed matter physics
• Research Institution: Tohoku University
• Principal Investigator: SASAKI Kazuo 東北大学, 工学研究科, 教授 (50205837)
• Co-Investigator(Kenkyū-buntansha): 吉留 崇 東北大学, 工学研究科, 助教 (90456830)
• Project Period (FY): 2017-04-01 – 2020-03-31
• Keywords: F1-ATPase / 化学状態 / 相互作用ポテンシャル / ポアソン過程 / 統計解析

Outline of Final Research Achievements

The rotor of a protein motor F1-ATPase can be forcefully rotated by manipulating a magnetic field if a magnetic nanoparticle is attached to the rotor. An algorithm is proposed that can infer the fundamental interaction producing the rotational motion of this motor, by statistically analyzing a time course of the rotation angle of the rotor obtained experimentally. A similar algorithm proposed earlier failed to infer the interaction in certain rages of rotation angle. In contrast, our algorithm successfully inferred it in the whole rage of the angle in the analysis of time courses produced by computers based on a theoretical model of F1-ATPase.

Free Research Field

統計物理学

Academic Significance and Societal Importance of the Research Achievements

私たちの体の中でエネルギーを蓄える普遍的な分子であるATPの合成に関わるタンパク質F1-ATPaseは，回転の力学的エネルギーを化学エネルギー変換することでATPを合成する．本研究成果により，F1-ATPaseが高いエネルギー変換効率で効率的に働く仕組みを解明するために必要な物理学的情報を，実験データから獲得するための道が開かれた．