Establishment of a method for diagnosing subtypes of Creutzfeldt-Jakob disease

2019 Fiscal Year Final Research Report

• Project/Area Number: 17K10022
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Infectious disease medicine
• Research Institution: University of Miyazaki
• Principal Investigator: MORI Tsuyoshi 宮崎大学, 医学部, 助教 (40426565)
• Co-Investigator(Kenkyū-buntansha): 佐藤 克也 長崎大学, 医歯薬学総合研究科(保健学科), 教授 (70398147)
• Project Period (FY): 2017-04-01 – 2020-03-31
• Keywords: 孤発性CJD / QUIC法

Outline of Final Research Achievements

Sporadic Creutzfeldt-Jakob disease (sCJD), a majority of human prion diseases, is classified in different subtypes which contribute to the occurrence of distinct clinical-pathological phenotypes. In this study, we tested whether a modification of the real-time quaking-induced conversion (RT-QUIC) assay, a sensitive diagnostic test for prion disease, could distinguish subtypes of sCJD. We prepared various PrP peptides and used them in the QUIC assay to derive those that show a promoting or inhibiting effect. RT-QUICs were performed with brain homogenates from several types of prion disease patients. As a result, by using several PrP peptide, there were slight differences in the RT-QUIC signaling between specimens. Furthermore, the use of multiple peptides further affected the RT-QUIC signal between specimens.

Free Research Field

分子生物学

Academic Significance and Societal Importance of the Research Achievements

急速な神経細胞変性をきたす致死性の疾患、プリオン病。その代表的な病気である孤発性クロイツフェルト・ヤコブ病（CJD）はタイプにより予後の進行状況が異なることから、発症早期にタイプを含めた孤発性CJDを正確に診断することが臨床現場から切望されている。ここでプリオンタイプによりRT-QUIC法でのPrP重要配列が異なることが示唆されたことは、予後診断法の確立において大きな意義があると考える。