Muscle regeneration system on Myokine

2022 Fiscal Year Final Research Report

• Project/Area Number: 17K13138
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Sports science
• Research Institution: Doshisha University (2022); Kumamoto University (2018); Nagasaki University (2017)
• Principal Investigator: Tsuchiya Yoshifumi 同志社大学, スポーツ健康科学部, 助教 (20795679)
• Project Period (FY): 2022-02-01 – 2023-03-31
• Keywords: サテライト細胞 / マイオカイン / 筋損傷 / 活性化

Outline of Final Research Achievements

Here, we investigated whether damaged myofibers influence the activation of satellite cells. Our findings revealed that satellite cells are directly activated by damaged myofiber-derived factors (DMDFs). DMDFs induced satellite cells to enter the cell-cycle; however, the cells stayed at the G1 phase and did not undergo S phase, while these cells were reversible to the quiescent state. Proteome analysis identified metabolic enzymes including GAPDH as DMDFs, whose recombinant proteins stimulated the activation of satellite cells. Satellite cells pre-exposed to the DMDFs demonstrated accelerated proliferation ex vivo. Treatment with recombinant DMDFs prior to muscle injury promoted expansion of the satellite cell population in vivo. Thus, our results indicate that DMDFs are not only a set of biomarkers for muscle damage but also acting as moonlighting proteins involved in satellite cell activation at the initial step of muscle regeneration.

Free Research Field

筋分子生物学

Academic Significance and Societal Importance of the Research Achievements

本研究で新規に同定したマイオカインがバイオマーカーとして、筋疾患の亢進を食い止める新たな指標や、創薬開発、トレーニング法の構築に繋がることが期待される。したがって、本研究成果に基づいた筋疾患の「予防医学的」な治療法やアスリートのための筋疲労回復促進など、関連する分野における研究を加速させ、臨床も含めた国内外における創薬事業やスポーツ啓発事業にも貢献するものと予想される。