2019 Fiscal Year Final Research Report
The development of diagnostic tool for severe fever with thrombocytopenia syndrome (SFTS) in a SFTS-endemic area in Japan.
| Project/Area Number |
17K15778
|
|---|
| Research Category |
Grant-in-Aid for Young Scientists (B)
|
| Allocation Type | Multi-year Fund |
|---|
| Research Field |
Laboratory medicine
|
|---|
| Research Institution | University of Miyazaki |
|---|
Principal Investigator |
|
|---|
| Project Period (FY) |
2017-04-01 – 2020-03-31
|
| Keywords | SFTS / diagnostic tools / Double-antigen ELISA |
|---|
| Outline of Final Research Achievements |
The aim of this study was to develop the useful diagnostic tool for severe fever with thrombocytopenia syndrome (SFTS) in daily clinical practice. We prepared recombinant SFTS virus-associated protein such as nucleoprotein (NP) and nonstructural S segment (NS) protein. Anti- recombinant NP (rNP) antibody was purified from serum of immunized rabbit with rNP. Double-antigen enzyme-linked immunosorbent assay (ELISA) using rNP detected anti-NP antibody in serum obtained from patients with early onset of SFTS. This assay showed that anti-NP antibody increased in serum within 3 or 4 days after SFTS virus infection. Although immunochromatographic assay (ICA) to detect SFTS virus antigen was also developed using anti-rNP rabbit antibody, the performance of ICA was unstable. In future study, it is necessary to improve the sensitivity of ICA for early diagnosis of SFTS. Double-antigen ELISA may be useful diagnostic tool for SFTS virus infection in a SFTS-endemic area in Japan.
|
| Free Research Field |
Infectious Diseases
|
| Academic Significance and Societal Importance of the Research Achievements |
致死率の高い感染症である重症熱性血小板減少症候群（SFTS）には有効な治療法がない．近年，SFTS発症早期にファビピラビルを投薬することにより，SFTS患者の回復が期待できることが示されている．本研究で確立したdouble-antigen ELISA法は，SFTSの早期診断に有用と考えられ，早期治療介入に貢献し死亡率を低減できる可能性がある．
|
