Identification of new DNA damages by DNA adductome analysis

2010 Fiscal Year Final Research Report

• Project/Area Number: 18101003
• Research Category: Grant-in-Aid for Scientific Research (S)
• Allocation Type: Single-year Grants
• Research Field: Risk sciences of radiation/Chemicals
• Research Institution: Kyoto University
• Principal Investigator: MATSUDA Tomonari Kyoto University, 大学院・工学研究科, 准教授 (50273488)
• Co-Investigator(Kenkyū-buntansha): SAEKI Kenichi 横浜薬科大学, 薬学部, 准教授 (60254306) ; TAKAMURA Takeji 神奈川工科大学, 応用化学科, 准教授 (50342910) ; KAWANISHI Masanobu 大阪府立大学, 産学官連携機構, 助教 (70332963)
• Project Period (FY): 2006 – 2010
• Keywords: DNAアダクトーム / DNA付加体 / 突然変異 / LC / MS / MS

Research Abstract

DNA, a principal genetic material, is damaged by attacking from various environmental factors. We developed a method named "DNA adductome" to detect DNA adducts comprehensively, and found that major DNA adducts in human organ are formed from lipid-oxidation. Additionally, we developed the method to quantify DNA adducts caused by alcohol drinking, acrylamide, nano-particles, air-pollutants, to clarify their health risk.

Research Products

• [Journal Article] Genotoxicity of colloidal fullerene c(60) (2011)
  • Author(s): Matsuda, S., Matsui, S., Shimizu, Y., Matsuda, T.
  • Journal Title: Environ Sci Technol 45 Pages: 4133-4138
  • Peer Reviewed
• [Journal Article] Acrylamide genotoxicity in young versus adult gpt delta male rats (2011)
  • Author(s): Koyama, N., Matsuda, T., Honma, M., et al.
  • Journal Title: Mutagenesis. in press
  • Peer Reviewed
• [Journal Article] Application of the DNA adductome approach to assess the DNA-damaging capability of in vitro micronucleus test-positive compounds (2011)
  • Author(s): Kato, K., Kawanishi, M., Yagi, T., Matsuda T., et al
  • Journal Title: Mutat Res 721 Pages: 21-26
  • Peer Reviewed
• [Journal Article] Effects of acetaldehyde inhalation in mitochondrial aldehyde dehydrogenase deficient mice (Aldh2-/-) (2010)
  • Author(s): Oyama, T., Nagayoshi, H., Matsuda, T., Kawamoto, T., et al.
  • Journal Title: Front Biosci (Elite Ed) 2 Pages: 1344-1354
  • Peer Reviewed
• [Journal Article] Anticipated Mutation Assay Using Single-molecule Real-time (SMRT TM) Sequencing Technology (2010)
  • Author(s): Matsuda, T.
  • Journal Title: Genes and Environment 32 Pages: 21-24
  • Peer Reviewed
• [Journal Article] DNA modifications by the omega-3 lipid peroxidation-derived mutagen 4-oxo-2-hexenal in vitro and their analysis in mouse and human DNA (2010)
  • Author(s): Kawai, K., Chou, P.H., Matsuda, T., Kasai, H., et al.
  • Journal Title: Chem Res Toxicol 23 Pages: 630-636
  • Peer Reviewed
• [Journal Article] Detection of lipid peroxidation-induced DNA adducts caused by 4-oxo-2(E)-nonenal and 4-oxo-2(E)-hexenal in human autopsy tissues (2010)
  • Author(s): Chou, P.H., Matsuda, T., et al.
  • Journal Title: Chem Res Toxicol 23 Pages: 1442-1448
  • Peer Reviewed
• [Journal Article] Genotoxicity of nano/microparticles in in vitro micronuclei, in vivo comet and mutation assay systems (2009)
  • Author(s): Totsuka, Y., Kawanishi, M., Wakabayashi, K., et al.
  • Journal Title: Part Fibre Toxicol 6 Pages: 23
  • Peer Reviewed
• [Journal Article] The genotoxic air pollutant 3-nitrobenzanthrone and its reactive metabolite N-hydroxy-3-aminobenzanthrone lack initiating and complete carcinogenic activity in NMRI mouse skin (2009)
  • Author(s): Schmeiser, H.H., Takamura-Enya, T., Arlt, V.M., et al
  • Journal Title: Cancer Lett 284 Pages: 21-29
  • Peer Reviewed
• [Journal Article] Increased formation of gastric N(2)-ethylidene-2'-deoxyguanosine DNA adducts in aldehyde dehydrogenase-2 knockout mice treated with ethanol (2009)
  • Author(s): Nagayoshi, H., Matsuda, T., et al
  • Journal Title: Mutat Res 673 Pages: 74-77
  • Peer Reviewed
• [Journal Article] Genotoxicity of 3, 6-dinitrobenzo[e]pyrene, a novel mutagen in ambient air and surface soil, in mammalian cells in vitro and in vivo (2009)
  • Author(s): Kawanishi, M., Yagi, T., et al
  • Journal Title: Mutagenesis 24 Pages: 279-284
  • Peer Reviewed
• [Journal Article] Quantification of 3-nitrobenzanthrone-DNA adducts using online column-switching HPLC-electrospray tandem mass spectrometry (2009)
  • Author(s): Gamboa da Costa, G., Takamura-Enya, T., Phillips, D.H., et al.
  • Journal Title: Chem Res Toxicol 22 Pages: 1860-1868
  • Peer Reviewed
• [Journal Article] Mutagenic specificity of N-acetoxy-3-aminobenzanthrone, a major metabolically activated form of 3-nitrobenzanthrone, in shuttle vector plasmids propagated in human cells (2008)
  • Author(s): Nishida, H., Kawanishi, M., Takamura-Enya, T., Yagi, T.
  • Journal Title: Mutat Res 654 Pages: 82-87
  • Peer Reviewed
• [Journal Article] Structural identification of DNA adducts derived from 3-nitrobenzanthrone, a potent carcinogen present in the atmosphere (2007)
  • Author(s): Takamura-Enya, T., Kawanishi, M., Yagi, T., Hisamatsu, Y.
  • Journal Title: Chem Asian J 2 Pages: 1174-1185
  • Peer Reviewed
• [Journal Article] DNA adduct formation and oxidative stress from the carcinogenic urban air pollutant 3-nitrobenzanthrone and its isomer 2-nitrobenzanthrone, in vitro and in vivo (2007)
  • Author(s): Nagy, E., Adachi, S., Takamura-Enya,T., Zeisig, M., Moller, L.
  • Journal Title: Mutagenesis 22 Pages: 135-145
  • Peer Reviewed
• [Journal Article] Increased formation of hepatic N2-ethylidene-2'-deoxyguanosine DNA adducts in aldehyde dehydrogenase 2-knockout mice treated with ethanol (2007)
  • Author(s): Matsuda, T., et al
  • Journal Title: Carcinogenesis 28 Pages: 2363-2366
  • Peer Reviewed
• [Journal Article] DNA adduct formation in human hepatoma cells treated with 3-nitrobenzanthrone : analysis by the (32)P-postlabeling method (2007)
  • Author(s): Kanno, T., Kawanishi, M., Takamura-Enya, T., Arlt, V.M., Phillips, D.H., Yagi, T.
  • Journal Title: Mutat Res 634 Pages: 184-191
  • Peer Reviewed
• [Journal Article] Application of the adductome approach to assess intertissue DNA damage variations in human lung and esophagus (2007)
  • Author(s): Kanaly, R.A., Matsui, S., Hanaoka, T., Matsuda, T.
  • Journal Title: Mutation Research 625 Pages: 83-93
  • Peer Reviewed
• [Journal Article] Mutagenicity and DNA adduct formation by the urban air pollutant 2-nitrobenzanthrone (2007)
  • Author(s): Arlt, V.M., Glatt, H., Gamboa da Costa, G., Reynisson, J., Takamura-Enya, T., Phillips, D.H.
  • Journal Title: Toxicol Sci 98 Pages: 445-457
  • Peer Reviewed
• [Journal Article] Mutagenic activities and physicochemical properties of selected nitrobenzanthrones (2006)
  • Author(s): Takamura-Enya, T., Suzuki, H., Hisamatsu, Y.
  • Journal Title: Mutagenesis 21 Pages: 399-404
  • Peer Reviewed
• [Journal Article] DNA damage and acute toxicity caused by the urban air pollutant 3-nitrobenzanthrone in rats : characterization of DNA adducts in eight different tissues and organs with synthesized standards (2006)
  • Author(s): Nagy, E., Adachi, S., Takamura-Enya T., Zeisig, M., Moller, L.
  • Journal Title: Environ Mol Mutagen 47 Pages: 541-552
  • Peer Reviewed
• [Journal Article] Increased DNA-damage-in ALDH2-deficient alcoholics (2006)
  • Author(s): Matsuda, T., Yabushita, H., Kanaly, R.A., Shibutani, S., Yokoyama, A.
  • Journal Title: Chem Res Toxicol 19 Pages: 1374-1378
  • Peer Reviewed
• [Presentation] 質量分析器、次世代DNAシーケンサーの変異原研究への応用可能性 (2010)
  • Author(s): 松田知成
  • Organizer: 日本環境変異原学会(JEMS)第39回大会
  • Place of Presentation: つくば
  • Year and Date: 20101116-20101117
• [Presentation] Significance of DNA adductome analysis in in vitro micronucleus test (2009)
  • Author(s): Muto, S., Kato, K., Yamamura, E., Kawanishi, M., Yagi, T., Matsuda T., Sugiyama, A., Uno, Y.
  • Organizer: In 10th International Conference on Environmental Mutagens (ICEM)
  • Place of Presentation: イタリア、フィレンツェ
  • Year and Date: 20090820-20090825
• [Presentation] ALDH2 genotype is critical for DNA adducts formation in mice treated with alcohol and acetaldehyde (2009)
  • Author(s): Matsuda T., Nagayoshi, H., Oka, M., Yukawa, Y., Hori, K., Kawamoto, T., Muto, M., Oyama, T.
  • Organizer: In 10th International Conference on Environmental Mutagens (ICEM)
  • Place of Presentation: イタリア,フィレンツェ
  • Year and Date: 20090820-20090825
• [Presentation] DNA adductomics : global survey of DNA damage in human tissues. (2008)
  • Author(s): Matsuda T., Kanaly, R.A., Chou, P.H.
  • Organizer: ECNIS WP6 workshop on new developments : biomarkers of complex mixtures and use of 'omics' technology.
  • Place of Presentation: クロアチア,ツァヴタット
  • Year and Date: 2008-09-21