2010 Fiscal Year Final Research Report
A Novel Genomic Approch for the Understanding of the Structure and Function of Chromosomes
| Project/Area Number |
18101007
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| Research Category |
Grant-in-Aid for Scientific Research (S)
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| Allocation Type | Single-year Grants |
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| Research Field |
基礎ゲノム科学
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| Research Institution | Tokyo Institute of Technology |
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Principal Investigator |
SHIRAHIGE Katsuhiko Tokyo Institute of Technology, 分子細胞生物学研究所, 教授 (90273854)
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| Co-Investigator(Kenkyū-buntansha) |
HIROTA Toru 財団法人癌研究会, 癌研究所・実験病理部, 部長 (50421368)
SUTANI Takashi 東京大学, 分子細胞生物学研究所, 助教 (30401524)
ITOH Takehiko 東京工業大学, 大学院・生命理工学研究科, 教授 (90501106)
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| Project Period (FY) |
2006 – 2010
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| Keywords | 染色体構築 / ChIP-chip / ChIP-seq / コヒーシン |
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| Research Abstract |
We have built up the system for the efficient analysis of chromosome structure of yeast species. Based on this system, we have identified many cross talks between chromosome functions and newly developed the new system to analyze chromosome structure of higher eukaryotes. The key method is to use in vitro transcription instead of PCR for the amplification of genomic fragment from higher eukaryotes that contain many and various classes of repetitive sequences, to avoid biased amplification of repeat. Using the system we unexpectedly found that cohesion, the protein complex essential for holding sister chromatids together until the onset of M-phase, co-localizes with CTCF, the protein essential for transcriptional insulation. Our data suggest that the cohesion complex itself is required for transcriptional insulation and CTCF is required for proper localization of cohesion complexes on chromosome.
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[Journal Article] Cohesin mediates transcriptional insulation by CCCTC-binding factor.2008
Author(s)
K.S.Wendt, K.Yoshida, T.Itoh, M.Bando, B.Koch, E.Schirghuber, S.Tsutsumi, G.Nagae, K.Ishihara, T.Mishiro, K.Yahata, F.Imamoto, H.Aburatani, M.Nakao, N.Imamoto, K.Maeshima, K.Shirahige, J.-M.Peters
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Journal Title
Nature (article) 451
Pages: 796-801
Peer Reviewed
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[Journal Article] Rad51 suppresses gross chromosomal rearrangement at centromere in Schizosaccharomyces pombe.2008
Author(s)
K.Nakamura, A.Okamoto, Y.Katou, C.Yadani, T.Shitanda, C.Kaweeteerawat, T.S.Takahashi, T.Itoh, K.Shirahige, H.Masukata, T.Nakagawa
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Journal Title
EMBO J. 27
Pages: 3036-3046
Peer Reviewed
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[Journal Article] Identification of novel androgen response genes in prostate cancer cells by coupling chromatin immunoprecipitation and genomic microarray analysis.2007
Author(s)
K.Takayama, K.Kaneshiro, S.Tsutsumi, K.Horie-Inoue, K.Ikeda, T.Urano, N.Ijichi, Y.Ouchi, K.Shirahige, H.Aburatani, S.Inoue
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Journal Title
Oncogene. 26
Pages: 4453-4463
Peer Reviewed
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[Journal Article] Protein Phosphatase 2A protects centromeric sister chromatid cohesion.2006
Author(s)
C.G.Riedel, V.L.Katis, Y.Katou, S.Mori, T.Itoh, W.Helmhart, M.Galova, M.Petronczki, J.Gregan, B.Cetin, I.Mudrak, E.Ogris, K.Mechtler, L.Pelletier, F.Buchholz, K.Shirahige, K.Nasmyth.
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Journal Title
Nature (Article) 441
Pages: 53-61
Peer Reviewed
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