Novel pathways of electro -chemical signals at membrane proteins

2008 Fiscal Year Self-evaluation Report

• Project/Area Number: 18209007
• Research Category: Grant-in-Aid for Scientific Research (A)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: General physiology
• Research Institution: Osaka University (2008-2009); National Institutes of Natural Sciences Okazaki Research Facilities (2006-2007)
• Principal Investigator: OKAMURA Yasushi Osaka University, 大学院・医学系研究科, 教授 (80201987)
• Project Period (FY): 2006 – 2008
• Keywords: イオンチャネル / 酵素 / シグナル伝達 / 生体分子 / 蛋白質

Research Abstract

電位センサ-とホスファタ-ゼを併せ持つ電位センサ-ドメイン蛋白と、電位センサ-ドメインのみから成りプロトンチャネル活性を示す蛋白など新たに同定された電位感受性蛋白による膜電位シグナル伝達のメカニズムと生理的意義を明らかにする

Research Products

• [Journal Article] Enzyme domain affects the movement of the voltage sensor in ascidian and zebrafish VSPs (2008)
  • Author(s): Hossain MI , Iwasaki H, Okochi Y, Chahine, M, Higashijima S, Nagayama K & Okamura Y.
  • Journal Title: J Biol Chem 283 Pages: 18248-18259
• [Journal Article] Multimeric nature of voltage-gated proton channels (2008)
  • Author(s): Koch HP, Kurokawa T, Okochi Y, Sasaki M, Okamura Y & Larsson HP.
  • Journal Title: Proc Natl Acad Sci USA 105(26) : 9111-6
• [Journal Article] A voltage-sensing phosphatase, Ci-VSP, which shares sequence identity with PTEN, dephosphorylates phosphatidylinositol 4, 5-bisphosphate (2008)
  • Author(s): Iwasaki H, Murata Y, Kim Y, Hossain MI, Worby CA, Dixon JE, McCormack T, Sasaki T & Okamura Y.
  • Journal Title: Proc Natl Acad Sci U S A 105 Pages: 7970-7975
• [Journal Article] Improving membrane voltage measurements using FRET with new fluorescent proteins (2008)
  • Author(s): Tsutsui H, Karasawa S, Okamura Y & Miyawaki A
  • Journal Title: Nature Methods 8 Pages: 683-5
• [Journal Article] Depolarization activates the phosphoinositide phosphatase Ci-VSP, as detected in Xenopus oocytes coexpressing sensors of PIP2 (2007)
  • Author(s): Murata Y & Okamura Y
  • Journal Title: J Physiol 583 Pages: 875-889
• [Journal Article] Biodiversity of voltage sensor domain proteins (2007)
  • Author(s): Okamura Y
  • Journal Title: (review) Pflugers Archiv 454(3) Pages: 361-71
• [Journal Article] A voltage sensor-domain protein is a voltage-gated proton channel (2006)
  • Author(s): Sasaki M, Takagi M & Okamura Y
  • Journal Title: Science 312(5773) Pages: 589-92
• [Presentation] VSOP protein lacking the C-terminal half of S4-like segment retains proton permeation (2009)
  • Author(s): 黒川竜紀、高木正浩、坂田宗平、大河内善史、岡村康司
  • Organizer: Biophysical society meeting
  • Place of Presentation: (ポスタ-)
  • Year and Date: 2009-03-03
• [Presentation] 電位依存性プロトンチャネルの動作機構 (2008)
  • Author(s): 黒川竜紀、コッホハンス、高木正浩、大河内善史、佐々木真理、ラ-ソンピ-タ-、岡村康司
  • Organizer: 第32回日本分子生物学会年会 第82回日本生化学会大会合同大会
  • Place of Presentation: 神戸(口頭)
  • Year and Date: 2008-12-12
• [Presentation] Biochemical characterization of voltage-gated proton channel, VSOP, in phagocytes (2008)
  • Author(s): Okochi Y, Sasaki M, Iwasaki H, Kurokawa T & Okamura Y
  • Organizer: 42nd Annual Scientific Meeting of the European Symposium of Clinical Investigation
  • Place of Presentation: Geneve, Switzerland(口頭)
  • Year and Date: 2008-03-29
• [Presentation] Voltage range for tuning of phosphatase of Ci-VSP as measured by two PIP2-sensors (2008)
  • Author(s): Hossain MI, Sakata S, Murata Y & Okamura Y
  • Organizer: 52th Biophysical Society Annual Meeting
  • Place of Presentation: Long Beach, USA(ポスタ-)
  • Year and Date: 2008-02-05
• [Presentation] Voltage-gated proton channels (2008)
  • Author(s): Okamura Y
  • Organizer: "Voltage-dependent proton channels : come of age", 16th IUPAB International Biophysics Congress, Biophysical Journal
  • Place of Presentation: Long Beach, USA(口頭)(vol.94, issue 2, 587-588)
  • Year and Date: 2008-02-05
• [Presentation] How do animals utilize signals of membrane potentials? lessons from two voltage-sensing proteins (2007)
  • Author(s): Okamura Y, Sasaki M, Kurokawa T, Okochi Y, Hossain MI, Iwasaki H, Murata Y & Higashijima S
  • Organizer: the 6th Okazaki Biology Conference : "Marine Biology"
  • Place of Presentation: Okazaki, Japan(口頭)
  • Year and Date: 2007-12-04
• [Presentation] Mechanisms of voltage-sensor domain proteins and insights into physiological significance (2007)
  • Author(s): Okamura Y
  • Organizer: Plenary Lecture, Annual Meeting of Neuroscience in Chile
  • Place of Presentation: Los Andes, Chile(口頭)
  • Year and Date: 2007-09-27
• [Book] Mol.Neurobiology 3rd Ed. Volumel(Mikoshiba K.)
  • Author(s): Okamura Y.
  • Publisher: Handbook of Neurochem(印刷中)
• [Book] 標準生理学第4版
  • Publisher: 医学書院