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2009 Fiscal Year Final Research Report

Mechanism of Biogenesis and Catalytic Function of Peptidyl Built-in Quinone Cofactors

Research Project

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Project/Area Number 18370043
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field Structural biochemistry
Research InstitutionOsaka University

Principal Investigator

TANIZAWA Katsuyuki  Osaka University, 産業科学研究所, 教授 (20133134)

Co-Investigator(Kenkyū-buntansha) OKAJIMA Toshihide  大阪大学, 産業科学研究所, 准教授 (10247968)
Project Period (FY) 2006 – 2009
Keywords銅アミン酸化酵素 / トパキノン / ビルトイン型キノン補酵素 / キノヘムプロテイン / 分子内チオエーテル架橋 / 翻訳後修飾 / X線結晶解析 / 酵素触媒機構
Research Abstract

Peptidyl built-in cofactors are produced by posttranslational modification of the cognate enzyme proteins, unlike ordinary cofactors which are mostly bio-synthesized from water soluble vitamins. In this study, we focused on two built-in quinone cofactor-containing enzymes, copper amine oxidase and quinohemoprotein amine dehydrogenase (QHNDH) that contain topa quinone (TPQ) and cysteine tryptophyl quinone (CTQ), respectively. For the reaction mechanism of copper amine oxidase, it has been demonstrated that the former reductive half-reaction is accompanied with the conformational change of TPQ. On the other hand, two proteins encoded close to the QHNDH genes, an [Fe-S]-cluster and S-adenosylmethionine-binding protein and a subtilisin-like protease, have been found to play essential roles for QHNDH biogenesis.

  • Research Products

    (16 results)

All 2010 2009 2008 2007 2006 Other

All Journal Article (8 results) (of which Peer Reviewed: 5 results) Presentation (5 results) Book (2 results) Remarks (1 results)

  • [Journal Article] 分子内架橋構造をもちトリプトファン残基に由来するキノン補酵素の生合成機構2009

    • Author(s)
      岡島俊英, 谷澤克行
    • Journal Title

      化学と生物 47

      Pages: 522-524

  • [Journal Article] Further Insight into the Mechanism of Stereoselective Proton Abstraction by Bacterial Copper Amine Oxidase.2008

    • Author(s)
      Taki, M., Murakawa, T., Nakamoto, T., Uchida, M., Hayashi, H., Tanizawa, K., Yamamoto, Y., Okajima, T.
    • Journal Title

      Biochemistry 47

      Pages: 7726-7733

    • Peer Reviewed
  • [Journal Article] Trapping of a Dopaquinone Intermediate in the TPQ Cofactor Biogenesis in a Copper-containing Amine Oxidase from Arthrobacter globiformis.2007

    • Author(s)
      Moore, R.H., Spies, M.A., Culpepper, M.B., Murakawa, T., Hirota, S., Okajima, T., Tanizawa K., Mure, M.
    • Journal Title

      J. Am. Chem. Soc. 129

      Pages: 11524-11534

    • Peer Reviewed
  • [Journal Article] nvolvement of a Putative [Fe-S]-Cluster-Binding Protein in the Biogenesis of Quinohemoprotein Amine Dehydrogenase.2006

    • Author(s)
      Ono, K., Okajima, T., Tani, M., Kuroda, S., Sun, D., Davidson, V.L., Tanizawa, K.
    • Journal Title

      J. Biol. Chem. 281

      Pages: 13672-13684

    • Peer Reviewed
  • [Journal Article] Kinetic and Structural Studies on the Catalytic Role of the Aspartic Acid Residue Conserved in Copper Amine Oxidase.2006

    • Author(s)
      Chiu, Y.-C., Okajima, T., Murakawa, T., Uchida, M., Taki, M., Hirota, S., Kim, M., Hayashi, H., Yamamoto, Y., Tanizawa, K.
    • Journal Title

      Biochemistry 45

      Pages: 4105-4120

    • Peer Reviewed
  • [Journal Article] Quantum Mechanical Hydrogen Tunneling in Bacterial Copper Amine Oxidase Reaction. Biochem.2006

    • Author(s)
      Murakawa, T., Okajima, T., Kuroda, S., Nakamoto, T., Taki, M., Yamamoto, Y., Hayashi, H., Tanizawa, K.
    • Journal Title

      Biophys. Res. Commun. 342

      Pages: 414-423

    • Peer Reviewed
  • [Journal Article] 新しいビルトイン型キノン補酵素CTQと分子内チオエーテル架橋構造-その特異な構造形成機構の解明に向けて-.2006

    • Author(s)
      谷澤克行
    • Journal Title

      酵素工学ニュース 56

      Pages: 5-9

  • [Journal Article] X線結晶解析と反応速度論的解析に基づく銅アミン酸化酵素の触媒機構.2006

    • Author(s)
      岡島俊英, 谷澤克行
    • Journal Title

      日本応用酵素協会誌 41

      Pages: 1-7

  • [Presentation] Acid-Base Chemistry in the Reductive Half-Reaction of Copper Amine Oxidase from Arthrobacter globiformis2010

    • Author(s)
      Murakawa, T., Hayashi, H., Okajima, T., Tanizawa, K.
    • Organizer
      Gordon Research Conference on Protein Cofactors, Radicals and Quinones
    • Place of Presentation
      Ventura, CA, USA
    • Year and Date
      20101024-20101029
  • [Presentation] X-ray Crystallographic Evidence for Conformational Change of Topaquinone in the Reductive Half-reaction of Copper Amine Oxidase from Arthrobacter globiformis, Gordon Research Conference on Protein Cofactors2010

    • Author(s)
      Hamaguchi, A., Okajima, T., Nakai, T. Tanizawa, K., Murakawa, T., Hayashi, H.
    • Organizer
      Radicals and Quinones
    • Place of Presentation
      Ventura, CA, USA
    • Year and Date
      20100124-20101029
  • [Presentation] 銅含有アミン酸化酵素のトパキノン補酵素生成におけるコンセンサス配列中のアスパラギン残基の役割2008

    • Author(s)
      元山暁仁、中西将太、黒田俊一、岡島俊英、谷澤克行
    • Organizer
      第31回日本分子生物学会年会/第81回日本生化学会大会合同大会(BMB2008)
    • Place of Presentation
      神戸国際会議場・神戸国際展示場(兵庫県)
    • Year and Date
      20081209-20081212
  • [Presentation] Involvement of an Iron Sulfur Protein and a Subtilisin-like Protease in the Biogenesis of Quinohemoprotein Amine Dehydrogenase2008

    • Author(s)
      Okajima, T., Ono, K., Tanizawa, K.
    • Organizer
      Japan-Holland Joint Seminar on New Aspects in Enzyme Science and Biotechnology
    • Place of Presentation
      九州大学西新プラザホール(福岡県)
    • Year and Date
      20080928-20080930
  • [Presentation] Conformational Flexibility of the TPQ Cofactor in Bacterial Copper Amine Oxidase, Gordon Research Conference on Protein Cofactors2008

    • Author(s)
      Okajima, T., Nakanishi, S., Murakawa, T., Hayashi, H., Tanizawa, K.
    • Organizer
      Radicals and Quinones
    • Place of Presentation
      Ventura, CA, USA
    • Year and Date
      20080120-20080125
  • [Book] Cofactors of Amine Oxidases: Copper Ion and Its Substitution and the 2, 4, 5-Trihydoxylphenylalanine Quinone (Chapter 3). In'Copper Amine Oxidases: Structures, Catalytic Mechanisms, and Role in Pathophysiology'(Taylor & Francis Group)2009

    • Author(s)
      Suzuki, S., Okajima, T., Tanizawa, K., Mure, M.
    • Total Pages
      19-38
    • Publisher
      CRC Press
  • [Book] Mechanism of TPQ Biogenesis in Prokaryotic Copper Amine Oxidase (Chapter 8). In'Copper Amine Oxidases: Structures, Catalytic Mechanisms, and Role in Pathophysiology'(Taylor & Francis Group)2009

    • Author(s)
      Okajima, T., Tanizawa, K.
    • Total Pages
      103-118
    • Publisher
      CRC Press
  • [Remarks]

    • URL

      http://www.sanken.osaka-u.ac.jp/labs/smb/project.html

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Published: 2011-06-18   Modified: 2016-04-21  

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