Elucidation of the invading mechanism of Neospora caninum into host cells by proteomics and its application for development of novel drugs and vaccines

2008 Fiscal Year Self-evaluation Report

• Project/Area Number: 18380180
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Applied veterinary science
• Research Institution: Obihiro University of Agriculture and Veterinary Medicine
• Principal Investigator: XUAN Xuenan Obihiro University of Agriculture and Veterinary Medicine, 原虫病研究センター, 教授 (10292096)
• Project Period (FY): 2006 – 2009
• Keywords: Neospora caninum / Toxoplasma gondii / プロテオーム / 感染機構 / 治療 / 予防

Research Abstract

本研究ではプロテオーム解析手法を用いて、ネオスポラ原虫の宿主細胞への吸着・侵入に関わるタンパク質の網羅的同定と新規ワクチン・薬剤標的分子の発掘を目的として実施する。

Research Products

• [Journal Article] Effects of a transferring antibody against Neospora caninum infection in a murine model (2009)
  • Author(s): Nishikawa, Y., Zhang, H., Huang, P., Zhang, G., Xuan, X.
  • Journal Title: Vet.Parasitol. 160 Pages: 60-65
  • Peer Reviewed
• [Journal Article] Proteomic analysis of calcium-dependent secretion in Toxoplasma gondii (2007)
  • Author(s): Kawase, O., Nishikawa, Y., Zhang, H., Zhang, G., Bannai, H., Jin, S., Lee, E.G., Xuan, X.
  • Journal Title: Proteomics 7 Pages: 3718-3725
  • Peer Reviewed
• [Journal Article] Identification of ribosomal phosphoprotein P0 of Neospora caninim as a potential common vaccine candidate for the control of both neosporosis and toxoplasmosis (2007)
  • Author(s): Zhang, H., Lee, E.G., Liao, M., Comaore, M., Zhang, G., Kawase, O., Fujisaki, K., Sugimoto, C., Nishikawa, Y., Xuan, X.
  • Journal Title: Mol. Biochem. Parasitol 153 Pages: 141-148
  • Peer Reviewed
• [Journal Article] Apical membrane antigen 1 is a cross-reactive antigen between Neospora caninum and Toxoplasma gondii, and anti-NcAMA1 antibody inhibit host cell invasion by both parasites (2007)
  • Author(s): Zhang, H., Compaore, M., Lee, E.G., Liao, M., Zhang, G., Sugimoto, C., Nishikawa, Y, Xuan, X.
  • Journal Title: Mol. Biochem. Paarasitol. 151 Pages: 205-212
  • Peer Reviewed
• [Journal Article] Common inheritance of chromosome Ia associated with clonal expansion of Toxoplasma gondii (2006)
  • Author(s): Khan, A., Bohme, U., Kelly, K. A., Adlem, E., Brooks, K., Simmonds, M., Mungall, K., Quail, M. A., Arrowsmith, C., Chillingworth, T., Churcher, C., Harris, D., Collins, M., Fosker, N., Fraser, A., Hance, Z., Jagels, K., Moule, S., Murphy, L., O'Neil, S., Rajandream, A., Saunders, D., Seeger, K., Whitehead, S., Mayr, T., Xuan, X., Watanabe, J., Suzuki, Y., Wakaguri, H., Sugano, S., Sugimoto, C., Paulsen,I., Mackey, A. J., Roos, D. S., Hall, N., Berriman, M., Barrell, B., Sibley, L. D., Ajioka, J. W.
  • Journal Title: Genome Res. 16 Pages: 1119-1125
  • Peer Reviewed