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2007 Fiscal Year Final Research Report Summary

Study on the peripheral mechanism of muscular mechanical hyperalgesia using the delayed onset muscle soreness as a model

Research Project

Project/Area Number 18390069
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field Environmental physiology (including Physical medicine and Nutritional physiology)
Research InstitutionNagoya University

Principal Investigator

MIZUMURA Kazue  Nagoya University, Res. Inst. Environ. Med, Professor (00109349)

Co-Investigator(Kenkyū-buntansha) KOZAKI Yasuko  Kinjo Galkuin Univ, Dept. Pharmacy, Associate Professor (20126882)
KATANOSAKA Kimiaki  Nagoya University, Res. Inst Environ Med, Assistant Professor (50335006)
HONDA Takashi  Fukushima Medical Univ, School of Nursing, Professor (20165608)
Project Period (FY) 2006 – 2007
Keywordsmuscular mechanical hyperalgesia / bradykinin / COX2 / nerve growth factor (NGF) / delayed onset muscle soreness / ATP / lengthening contraction / muscle thin-fiber receptors
Research Abstract

To clarify the mechanism for the muscular mechanical hyperalgesia, following three points were examined using a rat model of delayed onset muscle soreness that was induced by loading lengthening contraction (LC). 1. Change of sensitizing substances in the muscle that is released by compression of the muscle or during contraction, and sensitization of muscle C-fiber receptors by these substances. 2. Change in ion channels and reception expressed in muscle C-fiber sensory receptor neurons. 3. Change in physical (mechanical) environment of muscle sensory receptors in edematous muscle after LC. We found: 1. ATP was released by compression of the muscle, but its amount was not different between the muscles before and 2 days after LC. ATP 0.1-100 μM rather suppressed the response of muscle C-fiber receptors to the mechanical stimulation. Administration of bradykinin receptor antagonists, Cox inhibitors and anti-NGF (nerve growth factor) antibody at different time points around LC revealed that bradykinin through B2 receptors and Cox-2 were involved in triggering the process to mechanical hyperalgesia, and that NGF was involved in maintenance of mechanical hyperalgesia. Corresponding to these observations expression of Cox-2 mRNA and protein increased during 0 12 hr after LC whereas NGF mRNA increased during 12 hr 2 days after LC. NGF facilitated the mechanical response of muscle thin-fiber receptors recorded in vitro, supporting the role of NGF in mechanical hyperalgesia after LC. 2. Suppression subtractive hybridization analysis of mRNA in DRGs after exercise revealed upregulation of RNAs of 65 substances. RTPCR of two of them, pain-related annexin A2 and calbindin 1, showed significant increase 2 days after LC. 3. The muscle 2 days after LC was by about 5% heavier, suggesting existence of edema after I.C. Immunohistochemistry of aquaporin 1 and 4 in muscle showed no consistent change after LC.

  • Research Products

    (45 results)

All 2008 2007 2006 Other

All Journal Article (7 results) (of which Peer Reviewed: 2 results) Presentation (34 results) Book (3 results) Remarks (1 results)

  • [Journal Article] Compression-induced ATP release from rat skeletal muscle with and without lengthening contraction.2008

    • Author(s)
      TAGUCHI Toru
    • Journal Title

      Neuroscience Letters 434

      Pages: 277-281

    • Description
      「研究成果報告書概要(和文)」より
    • Peer Reviewed
  • [Journal Article] Compression-induced ATP release from rat skeletal muscle with and without lengthening contraction2008

    • Author(s)
      TAGUCHI, Toru
    • Journal Title

      Compression-induced ATP release from rat skeletal muscle with and without lengthening contraction 434(3)

      Pages: 277-281

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] Facilitated response of muscle receptors after eccentric contraction2008

    • Author(s)
      MIZUMURA, Kazue
    • Journal Title

      In : GRAVEN-NIELSEN T, ARENDT-NIELSEN L, Mense S eds. Fundamentals of Musculoskeletal Pain. Seattle : IASP Press

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] Change with age in muscular mechanical hyperalgesia after lengthening contraction in rats.2007

    • Author(s)
      TAGUCHI Toru
    • Journal Title

      Neuroscience Reseearch 57

      Pages: 331-338

    • Description
      「研究成果報告書概要(和文)」より
    • Peer Reviewed
  • [Journal Article] Change with age in muscular mechanical hyperalgesia after lengthening contraction in rats2007

    • Author(s)
      TAGUCHI, Toru
    • Journal Title

      Neuroscience Research 7(3)

      Pages: 31-338

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] Muscular pain mechanisms : Brief review with special consideration of delayed-onset muscle soreness2007

    • Author(s)
      MIZUMURA, Kazue
    • Journal Title

      In : ONOZUKA M, YEN C-T eds. Novel Trends in Brain Science, Tokyo : Springer

      Pages: 203-224

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] Single fiber recording technique using skin/muscle-nerve preparation-in vitro2006

    • Author(s)
      MIZUMURA, Kazue
    • Journal Title

      In : M. Kawatani eds. Methods for approaching pain mechanism. Tokyo : Shinko Koeki

    • Description
      「研究成果報告書概要(欧文)」より
  • [Presentation] Effects of COX-2 and NGF on mechanical hyperalgesia induced by exercise(DOMS)(遅発性筋痛(DOMS)におけるCOX-2およびNGFの関与について)2008

    • Author(s)
      妹尾 詩織
    • Organizer
      第85回日本生理学会大会
    • Place of Presentation
      東京
    • Year and Date
      20080325-27
    • Description
      「研究成果報告書概要(和文)」より
  • [Presentation] Upregulation of annexin A2 and calbindin 1 in dorsal root ganglion cells from rat with delayed onset muscle soreness2008

    • Author(s)
      小崎康子
    • Organizer
      第85回日本生理学会大会
    • Place of Presentation
      東京
    • Year and Date
      20080325-27
    • Description
      「研究成果報告書概要(和文)」より
  • [Presentation] Effects of COX-2 and NGF on mechanical hyperalgesia induced by exercise (DOMS)2008

    • Author(s)
      SENOO, Shiori
    • Organizer
      85th Annual Meeting of the Physiological Society of Japan
    • Place of Presentation
      Tokyo
    • Year and Date
      20080325-27
    • Description
      「研究成果報告書概要(欧文)」より
  • [Presentation] Upregulation of annexin A2 and calbindin 1 in dorsal root ganglion cells from rat with delayed onset muscle soreness2008

    • Author(s)
      KOZAKI, Yasuko
    • Organizer
      85th Annual Meeting of the Physiological Society of Japan
    • Place of Presentation
      Tokyo
    • Year and Date
      20080325-27
    • Description
      「研究成果報告書概要(欧文)」より
  • [Presentation] 遅発性筋痛の末梢神経機構の解析2007

    • Author(s)
      水村和枝
    • Organizer
      第62回日本体力医学会大会
    • Place of Presentation
      秋田
    • Year and Date
      20070914-16
    • Description
      「研究成果報告書概要(和文)」より
  • [Presentation] Analysis of peripheral neural mehanism of delayed onset muscle soreness2007

    • Author(s)
      MIZUMURA, Kazue
    • Organizer
      The 62nd Japanese Society of Physical Fitness and Sports Medicine
    • Place of Presentation
      Akita
    • Year and Date
      20070914-16
    • Description
      「研究成果報告書概要(欧文)」より
  • [Presentation] Adenosine triphosphate release induced by non-damaging mechanical stimulation from the muscle untreated and hyperalgesic muscle 2 days after lengthening contraction2007

    • Author(s)
      MIZUMURA Kazue
    • Organizer
      Seventh World Congress on Myofascial Pain and Fibromyalgia Syndrome
    • Place of Presentation
      Washington, DC, U.S.A
    • Year and Date
      20070819-23
    • Description
      「研究成果報告書概要(和文)」より
  • [Presentation] Clinical application of a muscle pain model: eccentric exercise, muscle pain, and C-fiber response augmentation2007

    • Author(s)
      MIZUMURA Kazue
    • Organizer
      Seventh World Congress on Myofascial Pain and Fibromyalgia Syndrome
    • Place of Presentation
      Washington, DC, U.S.A
    • Year and Date
      20070819-23
    • Description
      「研究成果報告書概要(和文)」より
  • [Presentation] Adenosine triphosphate release induced by non-damaging mechanical stimulation from the muscle untreated and hyperalgesic muscle 2 days after lengthening contraction2007

    • Author(s)
      MIZUMURA, Kazue
    • Organizer
      Myopain 2007 (Seventh World Congress on Myofascial Pain and Fibromyalgia Syndrome)
    • Place of Presentation
      Washington, DC, U.S.A
    • Year and Date
      20070819-23
    • Description
      「研究成果報告書概要(欧文)」より
  • [Presentation] Clinical application of a muscle pain model : eccentric exercise, muscle pain, and C-fiber response augmentation2007

    • Author(s)
      MIZUMURA, Kazue
    • Organizer
      Myopain 2007 (Seventh World Congress on Myofascial Pain and Fibromyalgia Syndrome)
    • Place of Presentation
      Washington, DC, U.S.A
    • Year and Date
      20070819-23
    • Description
      「研究成果報告書概要(欧文)」より
  • [Presentation] ATP release induced by the mechanical stimulation from the mechanically hyperalgesic muscle 2 days after lengthening contraction2007

    • Author(s)
      水村和枝
    • Organizer
      第84回日本生理学会大会
    • Place of Presentation
      大阪
    • Year and Date
      20070320-22
    • Description
      「研究成果報告書概要(和文)」より
  • [Presentation] Effect of nonsteroidal arti-inflammatory drugs(NSAIDS)and bradykinin(BK)receptor antagonists on mechanical hyperalgesia induced by exercise(DOMS)2007

    • Author(s)
      松田 輝
    • Organizer
      第84回日本生理学会大会
    • Place of Presentation
      大阪
    • Year and Date
      20070320-22
    • Description
      「研究成果報告書概要(和文)」より
  • [Presentation] Randall-Selitto device measures the mechanical withdrawal thresholds of different tissues in rats depending on its probe diameter2007

    • Author(s)
      那須輝顕
    • Organizer
      第84回日本生理学会大会
    • Place of Presentation
      大阪
    • Year and Date
      20070320-22
    • Description
      「研究成果報告書概要(和文)」より
  • [Presentation] ATP release induced by the mechanical stimulation from the mechanically hyperalgesic muscle 2 days after lengthening contraction2007

    • Author(s)
      MIZUMURA, Kazue
    • Organizer
      84th Annual Meeting of the Physiological Society of Japan
    • Place of Presentation
      Osaka
    • Year and Date
      20070320-22
    • Description
      「研究成果報告書概要(欧文)」より
  • [Presentation] Effect of nonsteroidal anti-inflammatory drugs (NSAIDS) and bradykinin (BK) receptor antagonists on mechanical hyperalgesia induced by exercise (DOMS)2007

    • Author(s)
      MATSUDA, Teru
    • Organizer
      84th Annual Meeting of the Physiological Society of Japan
    • Place of Presentation
      Osaka
    • Year and Date
      20070320-22
    • Description
      「研究成果報告書概要(欧文)」より
  • [Presentation] Randall-Selitto device measures the mechanical withdrawal thresholds of different tissues in rats depending on its probe diameter2007

    • Author(s)
      NASU, Teruaki
    • Organizer
      84th Annual Meeting of the Physiological Society of Japan
    • Place of Presentation
      Osaka
    • Year and Date
      20070320-22
    • Description
      「研究成果報告書概要(欧文)」より
  • [Presentation] Mechanism of exercise-induced muscular hyperalgesia2007

    • Author(s)
      MIZUMURA Kazue
    • Organizer
      Neuroplasticity and Learning in Chronic Pain
    • Place of Presentation
      Schwetzingen, Germany
    • Year and Date
      2007-11-15
    • Description
      「研究成果報告書概要(和文)」より
  • [Presentation] 筋性疼痛の最近の理解2007

    • Author(s)
      水村和枝
    • Organizer
      日本ペインクリニック学会第41回大会ランチョンセミナー9
    • Place of Presentation
      横浜
    • Year and Date
      2007-07-07
    • Description
      「研究成果報告書概要(和文)」より
  • [Presentation] Present understanding of muscular pain mechanism2007

    • Author(s)
      MIZUMURA, Kazue
    • Organizer
      The 41st Annual Meeting of Japan Society of Pain Clinicians, Luncheon Seminar 9
    • Place of Presentation
      Yokohama
    • Year and Date
      2007-07-07
    • Description
      「研究成果報告書概要(欧文)」より
  • [Presentation] Facilitated response of muscle receptors afer lengthening contraction2007

    • Author(s)
      MIZUMURA Kazue
    • Organizer
      IASP Research Symposium 2007: Fundamentals of Musculoskeletal Pain
    • Place of Presentation
      Aalborg, Denmark
    • Year and Date
      2007-05-07
    • Description
      「研究成果報告書概要(和文)」より
  • [Presentation] Facilitated response of muscle receptors after lengthening contraction2007

    • Author(s)
      MIZUMURA, Kazue
    • Organizer
      IASP Research Symposium 2007 : Fundamentals of Musculoskeletal Pain
    • Place of Presentation
      Aalborg, Denmark
    • Year and Date
      2007-05-07
    • Description
      「研究成果報告書概要(欧文)」より
  • [Presentation] Effects of non-steroidal anti-inflammatory drugs and bradykinin(BK)antagonists on muscular mechanical hyperalgesia afer lengthening contraction(LC)in rats2006

    • Author(s)
      MIZUMURA Kazue
    • Organizer
      The 6th Congress of the Federation of Asian and Oceanian Physiological Societies
    • Place of Presentation
      Seoul, Korea
    • Year and Date
      20061015-18
    • Description
      「研究成果報告書概要(和文)」より
  • [Presentation] Effects of non-steroidal anti-inflammatory drugs and bradykinin (BK) antagonists on muscular mechanical hyperalgesia after lengthening contraction (LC) in rats2006

    • Author(s)
      MIZUMURA, Kazue
    • Organizer
      The 6th Congress of the Federation of Asian and Oceanian Physiological Societies
    • Place of Presentation
      Seoul, Korea
    • Year and Date
      20061015-18
    • Description
      「研究成果報告書概要(欧文)」より
  • [Presentation] Change by aging in muscular mechanical hyperalgesia after lengthening contraction2006

    • Author(s)
      水村和枝
    • Organizer
      第29回日本神経科学大会
    • Place of Presentation
      京都
    • Year and Date
      20060719-21
    • Description
      「研究成果報告書概要(和文)」より
  • [Presentation] Change by aging in muscular mecha nical hyperalgesia after lengthening contraction2006

    • Author(s)
      MIZUMURA, Kazue
    • Organizer
      Neuroscience 2006
    • Place of Presentation
      Kyoto
    • Year and Date
      20060719-21
    • Description
      「研究成果報告書概要(欧文)」より
  • [Presentation] Periheral neural mechanism of exercise-induced muscle hyperalgesia2006

    • Author(s)
      MIZUMURA Kazue
    • Organizer
      From Molecules to Patients in Pain Research
    • Place of Presentation
      Nuernburg, Germany
    • Year and Date
      2006-09-22
    • Description
      「研究成果報告書概要(和文)」より
  • [Presentation] Periheral neural mechanism of exercise-induced muscle hyperalgesia2006

    • Author(s)
      MIZUMURA, Kazue
    • Organizer
      From Molecules to Patients in Pain Research
    • Place of Presentation
      Nuemburg, Germany
    • Year and Date
      2006-09-22
    • Description
      「研究成果報告書概要(欧文)」より
  • [Presentation] Periheral neural mechanism of exercise-induced muscle hyperalgesia2006

    • Author(s)
      MIZUMURA Kazue
    • Organizer
      Manheim University
    • Place of Presentation
      Heidelberg, Germany
    • Year and Date
      2006-09-20
    • Description
      「研究成果報告書概要(和文)」より
  • [Presentation] 筋性疼痛の基礎研究から2006

    • Author(s)
      水村和枝
    • Organizer
      日本ペインクリニック学会 第40回大会 ワークショップ
    • Place of Presentation
      神戸
    • Year and Date
      2006-07-14
    • Description
      「研究成果報告書概要(和文)」より
  • [Presentation] From the basic research on muscular pain2006

    • Author(s)
      MIZUMURA, Kazue
    • Organizer
      The 40th Annual Meeting of Japan Society of Pain Clinicians, Workshop
    • Place of Presentation
      Kobe
    • Year and Date
      2006-07-14
    • Description
      「研究成果報告書概要(欧文)」より
  • [Presentation] 痛みの神経シグナル 痛みはどのようにして感じられるのか?2006

    • Author(s)
      水村和枝
    • Organizer
      世界脳週間2006 脳研究の最前線 痛みの科学
    • Place of Presentation
      名古屋
    • Year and Date
      2006-04-22
    • Description
      「研究成果報告書概要(和文)」より
  • [Presentation] Neural signal of pain-How pain is sensed2006

    • Author(s)
      MIZUMURA, Kane
    • Organizer
      World Brain Week 2006, Frontier of Brain Research-Pain science
    • Year and Date
      2006-04-22
    • Description
      「研究成果報告書概要(欧文)」より
  • [Presentation] An approach for the mechanism of muscle pain using a model of delayed onset muscle soreness2006

    • Author(s)
      MIZUMURA Kazue
    • Organizer
      Cooperative Joint Conference on Neuroscience in Gifu
    • Place of Presentation
      岐阜
    • Year and Date
      2006-04-01
    • Description
      「研究成果報告書概要(和文)」より
  • [Presentation] An approach for the mechanism of muscle pain using a model of delayed onset muscle sore ness2006

    • Author(s)
      MIZUMURA, Kazue
    • Organizer
      Cooperative Joint Conference on Neuroscience in Gifu
    • Place of Presentation
      Gifu
    • Year and Date
      2006-04-01
    • Description
      「研究成果報告書概要(欧文)」より
  • [Book] Fundamentals of Musculoskeletal Pain(chapter 'Facilitated response of muscle receptors after eccentric contraction')2008

    • Author(s)
      MIZUMURA Kazue
    • Publisher
      IASP Press(印刷中)
    • Description
      「研究成果報告書概要(和文)」より
  • [Book] Novel Trends in Brain Science (Chapter 'Muscular pain mechanisms: Briefreview with special consideration of delayed-onset muscle soreness' in Section III. Pain)2007

    • Author(s)
      MIZUMURA Kazue
    • Total Pages
      203-232
    • Publisher
      Springer
    • Description
      「研究成果報告書概要(和文)」より
  • [Book] 皮膚・筋-神経標本からの単一神経記録による検討法:河谷正仁(編).痛み研究のアプローチ2006

    • Author(s)
      水村和枝
    • Total Pages
      88-96
    • Publisher
      真興交易医書出版部
    • Description
      「研究成果報告書概要(和文)」より
  • [Remarks] 「研究成果報告書概要(和文)」より

    • URL

      http://www.riem.nagoya-u.ac.jp/4/drof1/nr/index.html

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Published: 2010-02-04  

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