2009 Fiscal Year Final Research Report
The study of the mechanism of therapeutic efficacy of novel vaccines on Tuberculosis-infection and the induction of cytotoxic T cells by the vaccines
| Project/Area Number |
18390138
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Bacteriology (including Mycology)
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| Research Institution | Clinical Research Center, National Hospital Organization, Kinki-Chuo Chest Medical Center |
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Principal Investigator |
OKADA Masaji Clinical Research Center, National Hospital Organization, Kinki-Chuo Chest Medical Center, 臨床研究センター, 臨床研究センター長 (40160684)
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| Co-Investigator(Kenkyū-buntansha) |
YOSHIDA Shigeto 自治医科大学, 医学部, 講師 (10296121)
OHARA Naoya 国立感染症研究所, 免疫部 (70223930)
SUZUKI Katsuhiro 近畿中央胸部疾患センター, 臨床研究センター, 部長 (00206468)
INOUE Yoshikazu 近畿中央胸部疾患センター, 臨床研究センター, 部長 (90240895)
TSUYUGUCHI Kazunari 近畿中央胸部疾患センター, 臨床研究センター, 室長 (00359308)
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| Project Period (FY) |
2006 – 2009
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| Keywords | 感染症 / 免疫学 / 細菌 / 遺伝子 / 医療・福祉 |
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| Research Abstract |
(1)We have developed a novel therapeutic tuberculosis (TB) vaccine ; a combination of the DNA vaccines expressing mycobacterial heat shock protein 65 (HSP65) and interleukin 12 (IL-12) delivered by the hemagglutinating virus of Japan (HVJ)-envelope (HSP65+IL-12/HVJ). This vaccine induced CD8^+ cytotoxic T-cell specific for TB antigen.
(2)This vaccine exerted therapeutic efficacy in the TB-infected cynomolgus monkeys as well as mice.
(3)Granulysin augmented in vitro and in vivo induction of CTL. Thus, it was demonstrated that granulysin is CTL differentiation factor. Furthermore, in the presence of IL-6 or IL-2 the in vitro and in vivo induction by granulysin was synergistically enhanced.
(4)We established granulysin transgenic mice.
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