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2007 Fiscal Year Final Research Report Summary

Musculo-skeletal regeneration during distracionosteogenesis

Research Project

Project/Area Number 18390418
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field Orthopaedic surgery
Research InstitutionThe University of Tokushima

Principal Investigator

YASUI Natsuo  The University of Tokushima, GRADUATE SCHOOL, INSITUTE OF HEALTH BIOCIENCES, PROFESSOR (00157984)

Co-Investigator(Kenkyū-buntansha) MATSUURA Tetsuya  The University of Tokushima, UNIVERSITY MEDICAL AND DENTAL HOSPITAL, ASSISTANT PROFESSOR (30359913)
NIKAWA Takeshi  The University of Tokushima, GRADUATE SCHOOL, INSITUTE OF HEALTH BIOCIENCES, PROFESSOR (20263824)
SAIRYO Koichi  The University of Tokushima, GRADUATE SCHOOL, INSITUTE OF HEALTH BIOCIENCES, LECTURER (10304528)
Project Period (FY) 2006 – 2007
Keywordsdistraction osteogenesis / callus distraction / limb lenethening / osteoactivin / MMP-3 / transgenic mice / bone resorption / bone formation
Research Abstract

Distraction osteogenesis is a useful technique to study the response of bone to mechanical tension-stress in vivo. Using this technique, we here showed the unique response of bone to mechanical stress, especially the association between osteoactivin and MMP-3 expression. At 1 week after osteotomy of tibiae (the lag phase), mice were divided into two groups : the distraction osteogenesis group and non-distraction control group. In the first group, the tibiae were lengthened for 2 weeks (the distraction phase), followed by sustaining the length for 5 weeks (the consolidation phase). At 1 week after surgery, fibroblast-like cells (possible progenitors of osteoblasts or chondrocytes), which mainly expressed osteoactivin, were infiltrated into the osteotomy site. Interestingly, osteoactivin- and MMP-3-double positive fibroblast-like cells were accumulated in tibiae during the distraction phase. Consistent with immunohistochemical analysis, the levels of osteoactivin and MMP-3 transcripts in … More tibiae were significantly increased by osteotomy and distraction, respectively, in the distraction osteogenesis group, whereas no upregulated expression of MMP-3 was observed in the non-distraction group. To elucidate this difference in MMP-3 expression, we examined the shedding of osteoactivin during the distraction phase. Extracellular and intracellular fragments of osteoactivin were produced in distraction osteogenesis group, while they were hardly detected in the non-distraction group. These findings suggest that the shedding of osteoactivin is necessary for upregulation of MMP-3 expression in the lengthened segment. Finally, to address the role of osteoactivin-mediated MMP-3 expression in bone, osteoactivin-transgenic mice were subjected to this model. In osteoactivin-transgenic mice, bone resorption in the remodeling zone was significantly inhibited, compared with wild-type mice, whereas bone formation was not altered. We conclude that the shedding of osteoactivin functions as a mechanical tension-stress respondent inducer of MMP-3 for the fibroblast-like cells, and it mediates the inhibition of distracted callus resorption in the lengthened segment. Less

  • Research Products

    (33 results)

All 2008 2007 2006 Other

All Journal Article (27 results) (of which Peer Reviewed: 12 results) Presentation (2 results) Book (3 results) Patent(Industrial Property Rights) (1 results)

  • [Journal Article] Continuous local infusion of fibroblast growth factor-2 enhances consolidation of the bone segment lengthened by distraction osteogenesis in rabbit experiment.2008

    • Author(s)
      Abbaspour A
    • Journal Title

      Bone 42

      Pages: 98-106

    • Description
      「研究成果報告書概要(和文)」より
    • Peer Reviewed
  • [Journal Article] Treatment of dysplastic osteoarthritis with labral tear by Chiari pelvic osteotomy: outcomes after more than 10 years follow-up.2008

    • Author(s)
      Nakano S
    • Journal Title

      Arch Orthop Trauma Surg 128(1)

      Pages: 103-109

    • Description
      「研究成果報告書概要(和文)」より
    • Peer Reviewed
  • [Journal Article] Continuous infusion of insulin-like growth factor-I into the epiphysis of the tibia.2008

    • Author(s)
      Abbaspour A
    • Journal Title

      Int Orthop, (in press)

    • Description
      「研究成果報告書概要(和文)」より
    • Peer Reviewed
  • [Journal Article] Continuous local infusion of fibroblast growth factor-2 enhances consolidation of the bone segment lengthened by distraction osteogenesis in rabbit experiment2008

    • Author(s)
      Abbaspour, A
    • Journal Title

      Bone 42

      Pages: 98-106

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] Treatment of dysplastic osteoarthritis with labral tear by Chiari pelvic osteotomy : outcomes after more than 10 years follow-up2008

    • Author(s)
      Nakano, S
    • Journal Title

      Arch Orthop Trauma Surg 128(1)

      Pages: 103-109

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] Callus formation during healing of the repaired tendon-bone junction. A rat experimental model.2007

    • Author(s)
      Hibino N
    • Journal Title

      J Bone Joint Surg [Br] 89-B(11)

      Pages: 1539-1544

    • Description
      「研究成果報告書概要(和文)」より
    • Peer Reviewed
  • [Journal Article] Genetic association between the PRKCH gene encoding protein kinase Ceta isozyme and rheumatoid arthritis in the Japanese population.2007

    • Author(s)
      Takata Y
    • Journal Title

      Arthritis Rheum 56(1)

      Pages: 30-42

    • Description
      「研究成果報告書概要(和文)」より
    • Peer Reviewed
  • [Journal Article] SNPs in the KCNJ11-ABCC8 gene locus are associated with type 2diabetes and blood pressure levels in the Japanese population.2007

    • Author(s)
      Sakamoto Y
    • Journal Title

      J Hum Genet 52(10)

      Pages: 781-793

    • Description
      「研究成果報告書概要(和文)」より
    • Peer Reviewed
  • [Journal Article] Callus formation during healing of the repaired tendon-bone junction. A rat experimental model2007

    • Author(s)
      Hibino, N
    • Journal Title

      J Bone Joint Surg [Br] 89-B(11)

      Pages: 1539-1544

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] Genetic association between the PRKCH gene encoding protein kinase Ceta isozyme and rheumatoid arthritis in the Japanese population2007

    • Author(s)
      Takata, Y
    • Journal Title

      Arthritis Rheum 56(1)

      Pages: 30-42

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] SNPs in the KCNJ11-ABCC8 gene locus are associated with type 2 diabetes and blood pressure levels in the Japanese population2007

    • Author(s)
      Sakamoto, Y
    • Journal Title

      J Hum Genet 52(10)

      Pages: 781-793

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] Surgical correction of limb length difference2007

    • Author(s)
      Kawasaki, Y, Yasui, N
    • Journal Title

      Surgical techniques in Pediatric Orthopaedics(Medical View co.)

      Pages: 162-177

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] Treatment of knee deformities2007

    • Author(s)
      Kawasaki, Y, Yasui, N
    • Journal Title

      Joint Surgery 126(9)

      Pages: 52-61

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] Evaluation and correction of the deformities in lower limb in children(review)2007

    • Author(s)
      Yasui, N, Kawasaki, Y
    • Journal Title

      Journal of Japanese Pediatric Orhtopaedics Society 16(2)

      Pages: 280-283

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] Bisphosphonate Modulates Morphological and Mechanical Properties in Distraction Osteogenesis through Inhibition of Bone Resorption.2006

    • Author(s)
      Takahashi M
    • Journal Title

      Bone 39

      Pages: 573-581

    • Description
      「研究成果報告書概要(和文)」より
    • Peer Reviewed
  • [Journal Article] Ubiquitin ligase Cbl-b down-regulates bone formation through suppression of IGF-1 signaling in osteoblasts during denervation.2006

    • Author(s)
      Suzue N
    • Journal Title

      J Bone Miner Res 21(5)

      Pages: 722-734

    • Description
      「研究成果報告書概要(和文)」より
    • Peer Reviewed
  • [Journal Article] Spl Family of Transcription Factors Regulates the Human α2(XI) Collagen Gene(COL11A2)in Saos-2 Osteoblastic Cells.2006

    • Author(s)
      Goto T
    • Journal Title

      J Bone Miner Res 21(5)

      Pages: 663-673

    • Description
      「研究成果報告書概要(和文)」より
    • Peer Reviewed
  • [Journal Article] A novel type of EWS-CHOP fusion gene in myxoid liposarcoma.2006

    • Author(s)
      Matsui Y
    • Journal Title

      Biochemi Biophysi Res Commun 348

      Pages: 437-440

    • Description
      「研究成果報告書概要(和文)」より
    • Peer Reviewed
  • [Journal Article] JRAB/MICAL-L2 Is Junctional Rab13-binding Protein Mediating the Endocytic Recycling of Occcludin.2006

    • Author(s)
      Terai T
    • Journal Title

      Mol Biol Cell 17

      Pages: 2465-2475

    • Description
      「研究成果報告書概要(和文)」より
    • Peer Reviewed
  • [Journal Article] Overexpression of HMGA2-LPP fusion transcripts promotes expression of the alpha2 type XI collagen gene.2006

    • Author(s)
      Kubo T
    • Journal Title

      Biochem Biophys Res Commun 340(2)

      Pages: 476-481

    • Description
      「研究成果報告書概要(和文)」より
    • Peer Reviewed
  • [Journal Article] Bisphosphonate Modulates Morphological and Mechanical Properties in Distraction Osteogenesis through Inhibition of Bone Resorption2006

    • Author(s)
      Takahashi, M
    • Journal Title

      Bone 39

      Pages: 573-581

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] Ubiquitin ligase Cbl-b down-regulates bone formation through suppression of IGF-1 signaling in osteoblasts during denervation2006

    • Author(s)
      Suzue, N
    • Journal Title

      J Bone Miner Res 21(5)

      Pages: 722-734

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] Spl Family of Transcription Factors Regulates the Human a 2(X I) Collagen Gene(COL11A2)in Saos-2 Osteoblastic Cells2006

    • Author(s)
      Goto, T
    • Journal Title

      J Bone Miner Res 21(5)

      Pages: 661-673

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] A novel type of EWS-CHOP fusion gene in myxoid liposarcoma2006

    • Author(s)
      Matsui, Y
    • Journal Title

      Biochemi Biophysi Res Commun 348

      Pages: 437-440

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] JRABMDCAL-L2 Is Junctional Rab13-binding Protein Mediating the Endocytic Recycling of Occludin2006

    • Author(s)
      Terai, T
    • Journal Title

      Mol Biol Cell 17

      Pages: 2465-2475

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] Overexpression of HMGA2-LPP fusion transcripts promotes expression of the alpha 2 type XI collagen gene2006

    • Author(s)
      Kubo, T
    • Journal Title

      Biochem Biophys Res Commun 340(2)

      Pages: 476-481

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] Continuous infusion of insulin-like growth factor-I into the epiphysis of the tibia

    • Author(s)
      Abbaspour, A
    • Journal Title

      Int Orthop (in press)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Presentation] Workshop6:Changes of Gene Expression During Distraction Osteogenesis-A Mechanistic Update2007

    • Author(s)
      Yasui N
    • Organizer
      The 53rd Annual Meeting of the Orthopaedic Researth Society
    • Place of Presentation
      San Diego, California
    • Year and Date
      20070211-14
    • Description
      「研究成果報告書概要(和文)」より
  • [Presentation] Workshop6 : Changes of Gene Expression During Distraction Osteogenesis-A Mechanistic Update2007

    • Author(s)
      Yasui, N
    • Organizer
      53rd Annual Meeting of the Orthopaedic Research Society
    • Place of Presentation
      San Diego, California
    • Year and Date
      20070211-14
    • Description
      「研究成果報告書概要(欧文)」より
  • [Book] 下肢長不等に対する手術:延長術+矯正術2007

    • Author(s)
      川崎 賀照、安井 夏生
    • Total Pages
      162-177
    • Publisher
      小児整形外科手術テクニック(medical view社)
    • Description
      「研究成果報告書概要(和文)」より
  • [Book] 小児膝関節近傍の下肢変形に対する治療2007

    • Author(s)
      川崎 賀照、安井 夏生
    • Total Pages
      52-61
    • Publisher
      関節外科特集小児膝関節疾患・外傷の治療法、Vol.26 No.9
    • Description
      「研究成果報告書概要(和文)」より
  • [Book] 小児の下肢変形の評価と矯正(解説)2007

    • Author(s)
      安井 夏生、川崎 賀照
    • Total Pages
      280-283
    • Publisher
      日本小児整形外科学会雑誌、16号2巻
    • Description
      「研究成果報告書概要(和文)」より
  • [Patent(Industrial Property Rights)] 局所的骨成長促進剤および局所的骨成長促進用キット2006

    • Inventor(s)
      安井 夏生
    • Industrial Property Rights Holder
      徳島大学
    • Industrial Property Number
      特許、特願2006-000858
    • Filing Date
      2006-01-05
    • Description
      「研究成果報告書概要(和文)」より

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Published: 2010-02-04  

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