Bone and vascular signals during the repair process after bone and bone marrow injury

2009 Fiscal Year Final Research Report

• Project/Area Number: 18390422
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Orthopaedic surgery
• Research Institution: University of Occupational and Environmental Health, Japan
• Principal Investigator: SAKAI Akinori University of Occupational and Environmental Health, Japan, 医学部, 准教授 (90248576)
• Co-Investigator(Kenkyū-buntansha): NAKAMURA Toshitaka 産業医科大学, 医学部, 教授 (50082235) ; OKIMOTO Nobukazu 産業医科大学, 医学部, 助手 (70330991)
• Project Period (FY): 2006 – 2009
• Keywords: 骨髄損傷 / 骨芽細胞 / シグナル伝達 / ビタミンA / 血管 / VEGF / PECAM-1 / BMP-2

Research Abstract

The dis ruption of tyrosine kinase domain of flt-1, one of VEGF (vascular endothelial growth factor) receptors, disturbed osteoblast differentia tion and delayed the repair process after bone and bone marrow injury in mice. The production of alkaline phosphatase increased in osteoblasts co-cultured with endothelial cells. This increase was dose-dependently inhibited by PECAM-1 (platelet endothelial cell adhesion molecule-1) antibody. These findings demonstrated that vascular factors including VEGF and PECAM-1 significantly contributed to osteoblast development in the repairprocess afterbone and bone marrow injury.

Research Products

• [Journal Article] Elcatonin injections suppress systemic bone resorption without affecting cortical bone regeneration after drill-hole injuries in mice. (2009)
  • Author(s): Katae Y, Tanaka S, Sakai A, Nagashima M, Hirasawa H, Nakamura T
  • Journal Title: Journal of Orthopaedic Research 27巻12号 Pages: 1652-1658
  • Peer Reviewed
• [Journal Article] Climbing exercise enhances osteoblast differentiation and inhibits adipogenic differentiation with high expression of PTH/PTHrP receptor in bone marrow cells. (2008)
  • Author(s): Menuki K, Mori T, Sakai A, Sakuma M, Okimoto N, Shimizu Y, Kunugita N, Nakamura T
  • Journal Title: Bone 43巻3号 Pages: 613-620
  • Peer Reviewed
• [Journal Article] Genetic disruption of all NO synthase isoforms enhances BMD and bone turnover in mice in vivo: involvement of the renin-angiotensin system. (2008)
  • Author(s): Sabanai K, Tsutsui M, Sakai A, Hirasawa H, Tanaka S, Nakamura E, Tanimoto A, Sasaguri Y, Ito M, Shimokawa H, Nakamura T, Yanagihara N
  • Journal Title: Journal of Bone and Mineral Research 23巻5号 Pages: 633-643
  • Peer Reviewed
• [Journal Article] Flt-1 tyrosine kinase-deficient homozygous mice result in decreased trabecular bone volume with reduced osteogenic potential. (2007)
  • Author(s): Otomo H, Sakai A, Uchida S, Tanaka S, Watanuki M, Moriwaki S, Niida S, Nakamura T
  • Journal Title: Bone 40巻6号 Pages: 1494-1501
  • Peer Reviewed
• [Presentation] Skeletal loading enhances osteoblast differentiation and inhibits adipogenic differentiation in bone marrow cells. (2008)
  • Author(s): Sakai A, Menuki K, Nakamura T
  • Organizer: International Society for Fracture Repair, International Symposium on Biophysical Stimulation on Bone and Fracture Healing, Kyoto
  • Place of Presentation: Kyoto International Conference Center
  • Year and Date: 2008-11-26
• [Presentation] Vitamin A deficiency delays healing process after cortical bone and bone marrow injury. (2007)
  • Author(s): Tanaka K, Tanaka S, Sakai A, Hirasawa H, Katae Y, Nakura N, Menuki K, Yamane H, Sabanai K, Shimizu Y, Nakamura T
  • Organizer: The 17th Scientific Meeting International Bone and Mineral Society, Montreal, Canada
  • Place of Presentation: Montreal Convention Centre
  • Year and Date: 2007-06-28
• [Presentation] Changes of osteoblast development after unloading and reloading in murine models. International symposium (2006)
  • Author(s): Sakai A
  • Organizer: 第24回日本骨代謝学会
  • Place of Presentation: 東京都(東京ファッションタウン)
  • Year and Date: 2006-07-06
• [Book] 1章骨構造と機能細胞とその機能、最新整形外科学大系1運動器の生物学と生体力学(総編集越智隆弘、専門編集中村利孝、吉川秀樹) (2008)
  • Author(s): 酒井昭典
  • Total Pages: 7-11
  • Publisher: 中山書店