2007 Fiscal Year Final Research Report Summary
Improvement of sensitivity for chemiluminescnt detection system using complex
| Project/Area Number |
18550073
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Analytical chemistry
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| Research Institution | Kobe University |
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Principal Investigator |
SAITO Keiitsu Kobe University, Graduate School of Human Development and Environment, Professor (50162194)
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| Co-Investigator(Kenkyū-buntansha) |
KOMATSU Yu Kanazawa Institute of Tochnology, College of Environmental Engineering and Architechure, Professor (70339985)
KODAMATANI Hitoshi Nagashima University, Faculty of Science, Assistant Professor (30434468)
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| Project Period (FY) |
2006 – 2007
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| Keywords | Ruthenium romplex / Chemiluminescence / Thiophene derivative / Derivatization reagent / Phosphate |
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| Research Abstract |
The chemiluminescent reaction of tris(2,2'-bipyridine)ruthenium(III) with various compounds was examined. Five-membered heteroaromatic compounds produced intense emission. The chemiluminescent intensity increased with an increase in electron density of the aromatic ring. Aliphatic secondary amines having a carboxyl group at the α-position showed very intense chemiluminescence. The chemiluminescent reaction of tris(2,2'-bipyridine)ruthenium(III) with 2,5-dimethylthiophene was examined in detail. Tris(2,21-bipyridine)ruthenium(III) reacted with 2,5-dimethylthiophene in the ratio of 2:1. The reaction product was identified as 5-methyl-2-thiophenecarbaldeyde. From the simultaneous measurement of the chemiluminescent intensity and the oxidation potential and previously reported studies on the oxidative reaction of thiophene derivatives, the chemiluminescent reaction mechanism involving deprotonation was estimated. Since imino acids in human body fluid are important biomakers for various ill
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nesses, a sensitive simultaneous determination method for imino acids in human serum was developed. Four imino acids were successfully determined within 60 min.
To develop novel co-reactants for electrochemiluminesecence (ECL) immunoassays, preliminary studies on the tris(2,2'-bipyridine)ruthenium(II) ECL detection system using some compounds as co-reactants were performed. It was suggested that the sensitivity of the tris(2,2'-bipyridine)ruthenium(II)/ N,N-dipropyl-L-alanine system was comparable to that of the tris(2,2'-bipyridine)ruthenium(II)/ tripropylamine system, which has been widely used. Since N,N-dipropyl-L-alanine is a solid at ordinary temperature and its aqueous solution is neutral, it is ease of use compared to tripropylamine. A novel derivatization reagent, (2,4,5-trimethyl-3-thieny)acetic acid, for electrogenerated tris(2,2'-bipyridine)ruthenium (III) chemiluminescent detection was synthesized. Thirteen alkylphenols and bisphenol A in a mixed solution could be detected at ppb levels using high performance liquid chromatography. A sensitive determination method of phosphate in environmental water was developed. Molybdophosphate produced by the reaction of phosphate with molybdate in sulfuric acid formed an ion-pair with aliphatic tertiary amines. Phosphate was indirectly determined by the measurement of the aliphatic tertiary amines in the ion-pairs with tris(2,2'-bipyridine)ruthenium(III) chemiluminescent detection. Less
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[Journal Article] Novel Derivatization Reagent,(2,4,5-Trimethyl-3-thienyl)acetic Acid, for Electrogenerated Tris(2,2'-bipyridine)ruthenium(III) Chemiluminescence Detection2008
Author(s)
Hitoshi, KODAMATANI., Yu, KOMATSU., Shigeo, YAMAZAKI., Keiitsu, SAITO
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Journal Title
Chemistry Letters 37-1
Pages: 80-81
Description
「研究成果報告書概要(欧文)」より
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[Presentation] Determination of nicotine using tris(2,2'-bipyridine)ruthenium(III)chemiluminescence detection2007
Author(s)
Susumu, Tada., Emiko, Toyofuku., Hiroyuki, Kurata., Nanami, Ota., Hitoshi, Kodamatani., Yu, Komatsu., Shigeo, Yamazaki., Keiitsu, Saito
Organizer
56th Annual Meeting of the Japan Society for Analytical Chemistry
Place of Presentation
Tokushima
Year and Date
2007-09-18
Description
「研究成果報告書概要(欧文)」より
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[Presentation] Chemiluminescence detection of phosphate using ion-pair extraction2006
Author(s)
Nanami, Ota., Hitoshi, Kodamatani., Kaoru, Fujinaga., Yu, Komatsu., Shigeo, Yamazaki., Keiitsu, Saito
Organizer
Symposium on Solvent Extraction 2006
Place of Presentation
Kanazawa
Year and Date
2006-11-03
Description
「研究成果報告書概要(欧文)」より
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[Presentation] Sensitive and simultaneous determination of imino acids using HPLC with tris(2,2'-bipyridine)ruthenium(III)chemiluminescence detection2006
Author(s)
Hitoshi, Kodamatani., Nanami, Ota., Shigeo, Yamazaki., Yu, Komatsu., Kciitsu, Saito
Organizer
17th Conference of the Society for Chromatographic Sciences
Place of Presentation
Sendai
Year and Date
2006-11-02
Description
「研究成果報告書概要(欧文)」より
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[Presentation] Development of determination method for trimethylamines using tris(2,2'-bipyridine)ruthenium(III)chemiluminescentce detection2006
Author(s)
Yuki, Takada, Nanami, Ota, Hitoshi, Kodamatani, Yu, Komatsu, Shigeo, Yamazaki, Keiitsu, Saito
Organizer
55th Annual Meeting of the Japan Society for Analytical Chemistry
Place of Presentation
Osaka
Year and Date
2006-09-22
Description
「研究成果報告書概要(欧文)」より
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[Presentation] Chemiluminescence detection of heteroaromatic compounds using tris(2,2'-bipyridine)ruthenium(III)(3)-Application for analysis of alkylphenols2006
Author(s)
Hitoshi, Kodamatani, Keiitsu, Saito, Nobumitsu, Niina, Shigeo, Yamazaki, Yu, Komatsu
Organizer
67th, Symposium on Analytical Chemistry
Place of Presentation
Akita
Year and Date
2006-05-14
Description
「研究成果報告書概要(欧文)」より
