2007 Fiscal Year Final Research Report Summary
UItrastructural studies fir transition of lipid droplet in the egg during embryogenesis in Bombyx mori
| Project/Area Number |
18580051
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Applied entomology
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| Research Institution | Hamamatsu University School of Medicine |
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Principal Investigator |
YAMAHAMA Yumi Hamamatsu University School of Medicine, Faculty of Medicine, Instructor (90242784)
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| Co-Investigator(Kenkyū-buntansha) |
KUMAKIRI Yoko Hamamatsu University School of Medicine, Technical Staff Department, Technician (20397409)
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| Project Period (FY) |
2006 – 2007
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| Keywords | Bombyx mori / egg / embryogenesis / lipid droplet / lipid staining / transmission electron microscopy / high pressure freezing and freeze substitution met / insect |
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| Research Abstract |
Lipid droplets in insect eggs are considered one of the most important energy sources during late embryogenesis, however the process of their incorporation into the embryo is still unknown. The present study focused on the process of transition of lipid droplets from the extraembryonic yolk to the embryo of the silkworm Bombyx mori, using morphological and biochemical methods. We showed that there are three steps in the incorporation of lipid droplets from the extraembryonic yolk into the embryo ; the first step is translocation of lipid droplet directly before the blastokinesis stage, the second step is morphogenetic inclusion during dorsal closure stage, and the third step is ingestion of the extraembryonic yolk by the developing caterpillar before it's hatching. We demonstrated that lipid droplets and other component in the egg, which tended tolose by the conventional preparation method, were visible in electron microscopic observation using the high pressure freezing and freeze substitution method. We examined how the lipid droplets are incorporated into the embryonic tissues during early embryogenesis. Before blastokinesis stage, some of lipid droplets were fused and/or attached with developing rough endoplasmic reticulum, suggesting the incorporation of lipid from the cell surface and the formation of lipid droplet in the embryonic cell. After the dorsal closure stage, many yolk cells were existed in the midgut lumen, and fat bodies were in the coelom of developing caterpillar. Many lipid droplets were observed in the lumen of midgut enclosed with yolk cells, the intracellular of midgut, and the fat bodies.
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Research Products
(18 results)
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[Presentation] カイコ卵のTEM試料作製52007
Author(s)
熊切葉子, 村中祥悟, 山濱由美
Organizer
医学生物学電子顕微鏡技術学会第23回大会
Place of Presentation
北九州国際会議場
Year and Date
2007-05-20
Description
「研究成果報告書概要(和文)」より
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[Presentation] カイコ卵のTEM試料作製42006
Author(s)
由濱由美, 熊切葉子, 村中祥悟
Organizer
医学生物学電子顕微鏡技術学会第22回大会
Place of Presentation
クリエート浜松
Year and Date
2006-05-13
Description
「研究成果報告書概要(和文)」より
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