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2007 Fiscal Year Final Research Report Summary

Molecular and structural biostudies on streptococcalinyasion/infection mechanisms to host cells through degradation of extracellular matrices glyoceaminoglycans

Research Project

Project/Area Number 18580075
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Applied microbiology
Research InstitutionKyoto University

Principal Investigator

HASHIMOTO Wataru  Kyoto University, Grad. Schl.Agric, Associate Professor (30273519)

Project Period (FY) 2006 – 2007
KeywordsStreptococcus / Glycosaminoalycan / Polysaccharide lyase / Unsaturated glucuronyl hydrolase / Invasion / infection mechanism / X-ray crystallography / Sulfated saccharide / Extracellular matrix
Research Abstract

Some pathogenic microorganisms produce polysaccharide-degrading enzymes (lyases and hydrolases) to invade mammalian and/or plant cells. Mammalian glycosaminoglycans that form part of cell surface matrix are typical targets for microbial enzymes. Unsaturated glucuronyl hydrolase (UGL), which was genetically first identified in Bacillus sp. strain GL1, catalyzes the hydrolytic release of an unsaturated uronic acid from oligosaccharides produced through the reaction of the matrix-degrading polysaccharide lyases (e.g., hyaluronate and chondroitin lyases), suggesting that these enzymes function as a virulent factor in microbial infection. In this study, structure and function relationship of glycosaminoglycan-degrading enzymes, lyase and hydrolase, was analyzed.
Based on X-ray crystallography of enzyme-substrate complexes and site-directed mutagenesis, mechanisms for catalytic reaction and substrate recognition of hyaluronate lyase homologue (xanthan lyase) were clarified. A single tyrosine … More residue abstracts C5-proton of the uronate residue at subsite +1 and donates the proton to glycosidic bond to be cleaved.
In contrast with general glycoside hydrolases with the retention or inversion catalytic mechanism of an anomeric configuration, UGL uniquely triggers hydrolysis of vinyl ether groups in the unsaturated uronate residue but not of the glycosidic bond.
Recent complete genome sequence analyses indicate that a large number of microorganisms ranging from bacteria to fungi (over 70 species) have a UGL homologous gene in their genome. In the Carbohydrate-Active enZyme (CAZy) database, UGL and its homologues form a new family, GH-88. Microbial producers of UGL include pathogenic bacteria such as clostridia, streptococci, and vibrios. The enzyme homologous gene has been found especially in streptococci, Streptococcus agalactiae, Streptococcus pneumoniae, Streptococcus pyogenes, and Streptococcus suis. Unlike the bacillus UGL, streptococcal UGL acts on unsaturated chondroitin disaccharide with a sulfate group at C4 of GalNAc. Less

  • Research Products

    (29 results)

All 2008 2007 2006

All Journal Article (16 results) (of which Peer Reviewed: 8 results) Presentation (12 results) Book (1 results)

  • [Journal Article] Crystal structure of YihS in complex with D-mannose: Structural annotation of Escherichia coli and Salmonella enterica yihS-encoded proteins to an aldose-ketose isomerase2008

    • Author(s)
      Takafumi Itoh
    • Journal Title

      Journal of Molecular Biology 377

      Pages: 1443-1459

    • Description
      「研究成果報告書概要(和文)」より
    • Peer Reviewed
  • [Journal Article] Superchannel of bacteria: biological significance and new horizons2008

    • Author(s)
      Kousaku Murata
    • Journal Title

      Bioscience, Biotechnology and Biochemistry 72

      Pages: 265-277

    • Description
      「研究成果報告書概要(和文)」より
    • Peer Reviewed
  • [Journal Article] Crystal structure of YihS in complex with D-mannose : Structural annotation of Escherichia coli and Salmonella enterica yihS-encoded proteins to an aldose-ketose isomerase2008

    • Author(s)
      Takafumi, Itoh, Bunzo, Mikami, Wataru, Hashimoto, Kousaku, Murata
    • Journal Title

      Journal of Molecular Biology 377

      Pages: 1443-1459

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] Superchannel of bacteria : biological significance and new horizons2008

    • Author(s)
      Kousaku, Murata, Shigeyuki, Kawai, Bunzo, Mikami, Wataru, Hashimoto
    • Journal Title

      Bioscience, Biotechnology, and Biochemistry 72

      Pages: 265-277

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] Hydration of vinyl ether groups by unsaturated glycoside hydrolases and their role in bacterial pathogenesis2007

    • Author(s)
      Wataru Hashimoto
    • Journal Title

      International Microbiology 10

      Pages: 233-243

    • Description
      「研究成果報告書概要(和文)」より
    • Peer Reviewed
  • [Journal Article] A structural factor responsible for substrate recognition by Bacillus sp. GL1 xanthan lyase that acts specifically on pyruvated side chains of xanthan2007

    • Author(s)
      Yukie Maruyama
    • Journal Title

      Biochemistry 46

      Pages: 781-791

    • Description
      「研究成果報告書概要(和文)」より
    • Peer Reviewed
  • [Journal Article] Hydration of vinyl ether groups by unsaturated glycoside hydrolases and their role in bacterial pathogenesis2007

    • Author(s)
      Wataru, Hashimoto, Takafumi, Itoh, Yukie, Maruyama, Bunzo, Mikami, Kousaku, Murata
    • Journal Title

      International Microbiology 10

      Pages: 233-243

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] A structural factor responsible for substrate recognition by Bacillus sp. GL1 xanthan lyase that acts specifically on pyruvated side chains of xanthan2007

    • Author(s)
      Yukie, Maruyama, Bunzo, Mikami, Wataru, Hashimoto, Kousaku, Murata
    • Journal Title

      Biochemistry 46

      Pages: 781-791

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] Structure of unsaturated rhamnogalacturonyl hydrolase complexed with substrate2006

    • Author(s)
      Takafumi Itoh
    • Journal Title

      Biochemical and Biophysical Research Communications 347

      Pages: 1021-1029

    • Description
      「研究成果報告書概要(和文)」より
    • Peer Reviewed
  • [Journal Article] Crystal structure of unsaturated glucuronyl hydrolase complexed with substrate: Molecular insights into its catalytic reaction mechanism2006

    • Author(s)
      Takafumi Itoh
    • Journal Title

      Journal of Biological Chemistry 281

      Pages: 29807-29816

    • Description
      「研究成果報告書概要(和文)」より
    • Peer Reviewed
  • [Journal Article] A novel glycoside hydrolase family 105: The structure of family 105 unsaturated rhamnogalacturonyl hydrolase complexed with a disaccharide in comparison with family 88 enzyme complexed with the disaccharide2006

    • Author(s)
      Takafumi Itoh
    • Journal Title

      Journal of Molecular Biology 360

      Pages: 573-585

    • Description
      「研究成果報告書概要(和文)」より
    • Peer Reviewed
  • [Journal Article] Substrate recognition by unsaturated glucuronyl hydrolase from Bacillus sp. GL12006

    • Author(s)
      Takafumi Itoh
    • Journal Title

      Biochemical and Biophysical Research Communications 344

      Pages: 253-262

    • Description
      「研究成果報告書概要(和文)」より
    • Peer Reviewed
  • [Journal Article] Structure of unsaturated rhamnogalacturonyl hydrolase complexed with substrate2006

    • Author(s)
      Takafumi, Itoh, Akihito, Ochiai, Bunzo, Mikami, Wataru, Hashimoto, Kousaku, Murata
    • Journal Title

      Biochemical and Biophysical Research Communications 347

      Pages: 1021-1029

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] Crystal structure of unsaturated glucuronyl hydrolase complexed with substrate : Molecular insights into its catalytic reaction mechanism2006

    • Author(s)
      Takafumi, Itoh, Wataru, Hashimoto, Bunzo, Mikami, Kousaku, Murata
    • Journal Title

      Journal of Biological Chemistry 281

      Pages: 29807-29816

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] A novel glycoside hydrolase family 105 : The structure of family 105 unsaturated rhamnogalacturonyl hydrolase complexed with a disaccharide in comparison with family 88 enzyme complexed with the disaccharide2006

    • Author(s)
      Takafumi, Itoh, Akihito, Ochiai, Bunzo, Mikami, Wataru, Hashimoto, Kousaku, Murata
    • Journal Title

      Journal of Molecular Biology 360

      Pages: 573-585

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] Substrate recognition by unsaturated glucuronyl hydrolase from Bacillus sp. GL12006

    • Author(s)
      Takafumi, Itoh, Wataru, Hashimoto, Bunzo, Mikami, Kousaku, Murata
    • Journal Title

      Biochemical and Biophysical Research Communications 344

      Pages: 253-262

    • Description
      「研究成果報告書概要(欧文)」より
  • [Presentation] サルモネラ菌由来機能不明タンパク質YihSの構造・機能解析:ポスト構造ゲノミクスに向けて2008

    • Author(s)
      伊藤貴文
    • Organizer
      日本農芸化学会平成20年度大会
    • Place of Presentation
      名城大学
    • Year and Date
      2008-03-28
    • Description
      「研究成果報告書概要(和文)」より
  • [Presentation] Structural and functional analysis of Salmonella enterica YihS with function unknown : Road map for post-structural genomics2008

    • Author(s)
      Takafumi, Itoh, Bunzo, Mikami, Wataru, Hashimoto, Kousaku, Murata
    • Organizer
      Annual Meeting of Japan Society for Bioscience, Biotechnology, and Agrochemistry
    • Place of Presentation
      Meijo University
    • Year and Date
      2008-03-28
    • Description
      「研究成果報告書概要(欧文)」より
  • [Presentation] 連鎖球菌による宿主細胞外マトリクス・グリコサミノグリカンの分解2007

    • Author(s)
      橋本 渉
    • Organizer
      日本農芸化学会平成19年度大会
    • Place of Presentation
      東京農業大学
    • Year and Date
      2007-03-26
    • Description
      「研究成果報告書概要(和文)」より
  • [Presentation] 不飽和グルクロニルヒドロラーゼの基質認識と反応機構2007

    • Author(s)
      伊藤貴文
    • Organizer
      日本農芸化学会平成19年度大会
    • Place of Presentation
      東京農業大学
    • Year and Date
      2007-03-26
    • Description
      「研究成果報告書概要(和文)」より
  • [Presentation] Structure and function of glycosaminoglycan-degrading enzyme from streptococci2007

    • Author(s)
      Wataru, Hashimoto, Yukie, Maruyama, Takafumi, Itoh, Bunzo, Mikami, Kousaku, Murata
    • Organizer
      Annual Meeting of Japan Society for Bioscience, Biotechnology, and Agrochemistry
    • Place of Presentation
      Tokyo University of Agriculture
    • Year and Date
      2007-03-26
    • Description
      「研究成果報告書概要(欧文)」より
  • [Presentation] Substrate recognition and catalytic mechanism of bacterial unsaturated galacturonyl hydrolase responsible for degradation of plant cell walls2007

    • Author(s)
      Takafumi, Itoh, Akihito, Ochiai, Bunzo, Mikami, Wataru, Hashimoto, Kousaku, Murata
    • Organizer
      Annual Meeting of Japan Society for Bioscience, Biotechnology, and Agrochemistry
    • Place of Presentation
      Tokyo University of Agriculture
    • Year and Date
      2007-03-26
    • Description
      「研究成果報告書概要(欧文)」より
  • [Presentation] Bacterial unsaturated glycoside hydrolases as virulent factors: novel catalytic reaction mechanism2006

    • Author(s)
      伊藤貴文
    • Organizer
      日本生物物理学会平成18年度大会
    • Place of Presentation
      沖縄コンベンションセンター
    • Year and Date
      2006-11-14
    • Description
      「研究成果報告書概要(和文)」より
  • [Presentation] Bacterial unsaturated glycoside hydrolases as virulent factors novel catalytic reaction mechanism2006

    • Author(s)
      Takafumi, Itoh, Akihito, Ochiai, Bunzo, Mikami, Wataru, Hashimoto, Kousaku, Murata
    • Organizer
      Annual Meeting of Japan Society for Biophysics
    • Place of Presentation
      Okinawa Convention Center
    • Year and Date
      2006-11-14
    • Description
      「研究成果報告書概要(欧文)」より
  • [Presentation] 新規な糖質加水分解酵素ファミリー105:植物細胞壁に作用する枯草菌由来不飽和ガラクツロニルヒドロラーゼYteRの機能と高次構造2006

    • Author(s)
      伊藤貴文
    • Organizer
      日本農芸化学会平成18年度関西支部大会
    • Place of Presentation
      京都工芸繊維大学
    • Year and Date
      2006-10-01
    • Description
      「研究成果報告書概要(和文)」より
  • [Presentation] A novel glycoside family 105 : Function and structure of Bacillus subtilis unsaturated galacturonyl hydrolase YteR responsible for plant cell wall degradation2006

    • Author(s)
      Takafumi, Itoh, Akihito, Ochiai, Bunzo, Mikami, Wataru, Hashimoto, Kousaku, Murata
    • Organizer
      Annual Meeting of Japan Society for Bioscience, Biotechnology, and Agrochemistry, Kansai Branch
    • Place of Presentation
      Kyoto Institute of Technology
    • Year and Date
      2006-10-01
    • Description
      「研究成果報告書概要(欧文)」より
  • [Presentation] 動物細胞外マトリクスを分解する細菌酵素「不飽和グルクロニルヒドロラーゼ」の機能と構造2006

    • Author(s)
      伊藤貴文
    • Organizer
      日本農芸化学会平成18年度関西支部例会
    • Place of Presentation
      京都府立大学
    • Year and Date
      2006-05-27
    • Description
      「研究成果報告書概要(和文)」より
  • [Presentation] Bacterial enzyme for degrading mammalian extracellular matrix : Function and structure of unsaturataed glucuronyl hydrolase2006

    • Author(s)
      Takafumi, Itoh, Wataru, Hashimoto, Bunzo, Mikami, Kousaku, Murata
    • Organizer
      Regular Meeting of Japan Society for Bioscience, Biotechnology, and Agrochemistry, Kansai Branch
    • Place of Presentation
      Kyoto Prefectural University
    • Year and Date
      2006-05-25
    • Description
      「研究成果報告書概要(欧文)」より
  • [Book] 遺伝子から見た応用微生物学2008

    • Author(s)
      橋本 渉
    • Total Pages
      55-68
    • Publisher
      朝倉出版
    • Description
      「研究成果報告書概要(和文)」より

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Published: 2010-02-04  

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