2007 Fiscal Year Final Research Report Summary
Mechanism of inhibitory activity of lenthionine against platelet aggregation and its ex vivo effect
| Project/Area Number |
18580130
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Food science
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| Research Institution | Nihon University |
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Principal Investigator |
KUMAGAI Hitomi Nihon University, College of Bioresource Sciences, Department of Agricultural Chemistry, Associate Professor (20225220)
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| Project Period (FY) |
2006 – 2007
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| Keywords | Ienthionine / shiitake mushroom / platelet aggregation / cyclic sulfuric compound / ex vivo experiment |
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| Research Abstract |
Inhibiroy activity of lenthionine against signal transduction in platelets
Inhibitory activity of lenthionine, the most abundant shiitake flavor component, atainst platelet aggregation was examined. Lenthionine inhibited platelet aggregation induced by arachidonic acid and U-46619. The inhibitory activity of authentic lenthionine was almost similar to that of lenthionine in the essential oil from shiitake, indicating that lenthionine was the most active component in the essential oil.
Then, platelet was activated by using various agonists such as A23187, PMA, PAF, and ADP, to examine the inhibitory mechanizm of lenthionine. Lenthionine inhibited the aggreation of plateles induced by every agonists tested. This result indicates that lenthionie would not inhibit signal transduction cascade in platelets but inhibit adhesion and shape change of platelets, receptors for platelet activation, or phosphorylation of proteins.
Inhibition of ex vivo platelet aggregation by lenthionine
Lenthionine was orally administered to rats and blood was withdrawn after a certain interval to examine its ex vivo effect on platelet aggregation. Oral-administrated lenthionine significantly inhibited platelet aggregation 8-20 hours after the administration. Then, dose of lenthionine was varied to examine the minimum dose to exert the inhibitory activity against platelet aggregation. As a result, more than 1 mg/kg of lenthionine significantly inhibited platelet aggregation.
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Research Products
(8 results)
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[Presentation] Effect of lenthionine on activation of platelets2006
Author(s)
Shoichiro, Shimada, Kazuki, Kumagai, Yuka, Sato, Hitomi, Kumagai, Hidetoshi, Sakurai
Organizer
The 53rd Annual Meeting of the Japanese Society for Food Science and Technology
Place of Presentation
Nihon University
Year and Date
2006-08-29
Description
「研究成果報告書概要(欧文)」より
