2007 Fiscal Year Final Research Report Summary
Elucidation of the formation pathways of nitric oxide involved in the reduction of orally-ingested nitrite in vivo
| Project/Area Number |
18590235
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
General pharmacology
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| Research Institution | The University of Tokushima |
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Principal Investigator |
TSUCHIYA Koichiro The University of Tokushima, Institute of Health Biosciences, Department of Medical Pharmacology, Professor (70301314)
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| Co-Investigator(Kenkyū-buntansha) |
TAKIGUCHI Yoshiharu The University of Tokushima, Institute of Health Biosciences, Department of Clinical Pharmacology, Professor (40163349)
TAMAKI Toshiaki The University of Tokushima, Institute of Health Biosciences, Department of Pharmacology, 教授 (80179879)
KANEMATSU Yasuhisa The University of Tokushima, Institute of Health Biosciences, Department of Pharmacology, Associate Professor (90363142)
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| Project Period (FY) |
2006 – 2007
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| Keywords | nitric oxide / quercetin / nitrite / iron / EPR |
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| Research Abstract |
In this research, we investigated whether reducing agents such as quercetin and iron(II) facilitate nitric oxide (NO) gas formation from orally ingested nitrite in vivo study. When 3 mg/kg Na^<15>NO_2 was orally administered to rats with or without iron(II) or quercetin, Hb^<15>NO, which is indicative of systemic (15)^NO, was appeared in the blood, and the time to maximum blood concentration of Hb^<15>N0 was 15 min after nitrite or nitrite plus quercetin treatment, whereas nitrite plus iron(II) or nitrite plus iron(II) with quercetin shortened the time (10 min). Interestingly either iron(II), quercetin, or iron(II) plus quercetin did not affect the total amount of Hb^<15>NO generated from orally administered Na^<15>NO_2. However, systemic nitrite concentration was significantly decreased in the presence of iron(II) or iron(II) plus quercetin. These results may indicate that iron(II) is of critical importance to the generation of NO gas from nitrite, whereas quercetin provides little contribution in vivo under our experimental conditions.
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