2007 Fiscal Year Final Research Report Summary
Identification of glucocorticoid target genes involved in visceral obesity
Project/Area Number |
18590997
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Research Category |
Grant-in-Aid for Scientific Research (C)
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Allocation Type | Single-year Grants |
Section | 一般 |
Research Field |
Metabolomics
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Research Institution | University of Hyogo |
Principal Investigator |
KAJI Hidesuke University of Hyogo, College of Nursing Art & Science, Professor (90224401)
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Co-Investigator(Kenkyū-buntansha) |
FUKANO Chika University of Hyogo, College of Nursing Art & Science, Assistant Professor (00405367)
TANIDA Keiko University of Hyogo, College of Nursing Art & Science, Senior Assistant Professor (60405371)
KIMURA Yukari University of Hyogo, College of Nursing Art & Science, Associate (80438259)
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Project Period (FY) |
2006 – 2007
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Keywords | adipocyte / glucocorticoid / RNA interference (RNAi) / metabolic syndrome (MetS) / single nuculeotide polymorphism (SNP) / CCAAT / enhancer binding protein 8 (C / EBPδ) / neuropeptide Y (NPY) / NPY type 2 receptor (NPY2R) |
Research Abstract |
We investigated to clarify whether connective tissue growth factor, emerin, and calpain 1 are essential for the initial step of adipocyte differentiation, since glucocorticoid is very important for adipocyte differentiation, and mRNA expressions of these 3 molecules are induced by cocktail with dexamethasone during the initial adipocyte differentiation from 3T3-L1 cells, and they have their human ortholog as well as glucocorticoid responsive element on their 5'-flanking region of the gene. The RNA interference in each mRNA expression failed to change the adipocyte differentiation, suggesting that these molecules, at least by each single molecule, are not essential for the initial step of the adipocyte differentiation. Next, the association between metabolic syndrome (MetS) and CCAAT/enhancer binding protein δ (C/EBPδ) was investigated after informed consent in 172 persons for health checkup. It has been reported in the study using C/EBPδ knockout mouse that C/EBPδ mRNA induction by gluc
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ocorticoid during the initial adipocyte differentiation from 3T3-L1 cells is essential for visceral obesity. Our result indicated for the first time that genotype C/T in SNP rs15955 was more prevalent in dyslipidemia as well as impaired glucose tolerance (IGT) among phenotypes of MetS. Furthermore, neuropeptide Y (NPY)/NPY type 2 receptor (NPY2R) pathway is recently reported to be involved in high fat and sucrose diet-induced visceral obesity under certain stresses with stimulated glucocorticoid secretion. Therefore, we tested the association between MetS (IDF Asia criteria) and the 5'-flanking region of NPY2R gene SNPs with high minor allele frequency in Asian population. In 317 persons for health checkup participated in this study after informed consent, genotype '17 in SNP rs6857715 was more prevalent in MetS and IGT. Genotype AA in SNP rs6857530 was more prevalent in IGT. Further functional studies of the transcriptional control of these SNP regions will be required for the development of prevention strategy and drug discovery for MetS. Less
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Research Products
(51 results)
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[Journal Article] Chemerin enhances insulin signaling and potentiates insulin-stimulated glucose uptake in 3T3-L1 adipocytes2008
Author(s)
Takahashi M, Takahashi Y, Takahashi K, Zolotaryov FN, Hong KS, Kitazawa R, IidaK Okimura Y, Kaji H, Kitazawa S, Kasuga M, Chihara K.
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Journal Title
FEBS Lett 582(5)
Pages: 573-578
Description
「研究成果報告書概要(和文)」より
Peer Reviewed
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[Journal Article] Chemerin enhances insulin signaling and potentiates insulin-stimulated glucose uptake in 3T3-L1 adipocytes2008
Author(s)
Takahashi M, Takahashi Y, Takahashi K, Zolotaryov, FN, Hong, KS, Kitazawa R, IidaK, Okimura Y, Kaji H, Kitazawa S, Kasuga M, Chihara, K
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Journal Title
Description
「研究成果報告書概要(欧文)」より
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[Journal Article] Neither intravenous nor intracerebroventricular Administration of obestatin affects the secretion of GH, PRL, TSH and ACTH in rats2007
Author(s)
Yamamoto D, Ikehashi N, Daito R, Herningtyas, EH, Toda K, Kaji, H(9/11), Okimura Y, et. al.
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Journal Title
Regulatory Peptides 138
Pages: 141-144
Description
「研究成果報告書概要(欧文)」より
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[Journal Article] The N131S mutation in the von Hippel-Lindau gene in a Japanese family with pheochromocytoma and hemangioblastomas2006
Author(s)
Imanaka M, Iida K, Takahashi K, Tsuji K, Nishizawa H., Fukaoka H, Takeno R, Takahashi Y, Okimura Y, Kaii H, Chihara K
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Journal Title
Endocrine Journal 53
Pages: 819-827
Description
「研究成果報告書概要(和文)」より
Peer Reviewed
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[Journal Article] The N131S mutation in the von Hippel-Lindau gene in a Japanese family with pheochromocytoma and hemangioblastomas2006
Author(s)
Imanaka M, Iida K, Takahashi K, Tsuji K, Nishizawa, H., Fukaoka H, Takeno R, Takahashi Y, Okimura Y, Kaji H, Chihara, K
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Journal Title
Endocrine Journal 53
Pages: 819-827
Description
「研究成果報告書概要(欧文)」より
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[Presentation] IGF-I stimulates reactive oxygen species(ROS) production, thereby affecting insulin sensitivity in adipocytes2007
Author(s)
Fukuoka, H., Takahashi, Y., Iida, K., Kaji, H.(11/12), et. al.
Organizer
The Endocrine Society's 89th Annual Meeting
Place of Presentation
Toronto, Canada
Year and Date
2007-06-05
Description
「研究成果報告書概要(欧文)」より
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[Presentation] Growth hormone increase FoxOl protein levels and the expression of its target genes in adipose tissue2006
Author(s)
Fukuoka, H., Takahashi, Y., Okimura, Y., Kaji, H(10/11)., Chihara, K., et. al.
Organizer
The Third International Congress of GRS and IGF Society
Place of Presentation
Kobe
Year and Date
2006-11-12
Description
「研究成果報告書概要(和文)」より
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[Presentation] Growth hormone increase FoxO1 protein levels and the expression of its target genes in adipose tissue2006
Author(s)
Fukuoka, H., Takahashi, Y., Okimura, Y., Kaji, H.(10/11), Chihara, K., et. al.
Organizer
The Third International Congress of GRS and IGF Society
Place of Presentation
Kobe
Year and Date
2006-11-12
Description
「研究成果報告書概要(欧文)」より
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[Presentation] Difference in the frequency of polymorphism in the growth hormone receptor gene between Japanese and Caucasian populations2006
Author(s)
Iida, K., Takahashi, Y., Kaji, H., Okimura, Y., Nose, O., Chihara, K.
Organizer
The Endocrine Society's88th Annual Meeting
Place of Presentation
Boston,USA
Year and Date
2006-06-25
Description
「研究成果報告書概要(和文)」より
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[Presentation] Difference in the frequency of polymorphism in the growth hormone receptor gene between Japanese and Caucasian populations2006
Author(s)
Iida, K., Takahashi, Y., Kaji, H., Okimura, Y., Nose, O., Chihara, K
Organizer
The Endocrine Society's88th Annual Meeting
Place of Presentation
Boston, USA
Year and Date
2006-06-25
Description
「研究成果報告書概要(欧文)」より
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