2007 Fiscal Year Final Research Report Summary
Novel Roles of mast cells in Eosinophilic nasal polyps including aspirin sensitive nasal polyps
| Project/Area Number |
18591889
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Otorhinolaryngology
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| Research Institution | Nippon Medical School |
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Principal Investigator |
PAWANKAR Ruby Nippon Medical School, 医学部, Associate professor (00287674)
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| Co-Investigator(Kenkyū-buntansha) |
TAKIZAWA Ryuta Nippon Medical School, Otolaryngology, Assistant (10271347)
OZU Chika Nippon Medical School, Otolaryngology, Assistant (20328785)
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| Project Period (FY) |
2006 – 2007
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| Keywords | Nasal Polyps / Mast cell / MC(T) and MC(TC) / FcεRI / Eosinophils / MMP-9 / IL-13 / IgE |
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| Research Abstract |
The mechanisms of eosinophilic nasal polyps and aspirin induced nasal polyps is not well defined. Most studies have focused on investigating the epithelial cells and eosinophilic mediators. Mast cells are increased in the nasal polyps and are important inflammatory cells and are particularly increased in eosinophilic nasal polyps and aspirin sensitive nasal polyps and may play a role in the infiltration of eosinophils
In the present study, we used the the mast cell culture system we established to investigate the activating mechanisms of the nasal polyp mast cells. Upon stimulating nasal polyp mast cells with the FceRI antibody we investigated the levels of histamine, tryptase, IL-4, IL-5 TNF-alpha, IL-13 and TGF-beta by ELISA. Histamine, tryptase, IL-, IL-5, IL-13, TNF-alpha were increased in the cell supernatants of stimulated mast cells.
The levels of histamine and eosinophilic cationic protein that are proteins of mast cells and eosinophils respectively gad a good correlation. From the point of view of increased IL-4 and IL-13, these are related to the increased levels of IgE in nasal polyps. Histaimine from nasal polyp mast cells upregulates RANTES production from nasal epithelial cells and this in turn can induce increased eosinophil infiltration into the nasal polyp
MMP-9 also was increased in nasal polyp. TIMP-1 and TIMP-2 were less. Mast cells and eosinophils exprrssed MMP-9. This can induce the structural changes seen in eosinophilic nasal polyps
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