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2008 Fiscal Year Final Research Report

Molecular structure and function of water oxidation in Photosystem II : for application to hydrogen production

Research Project

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Project/Area Number 18770116
Research Category

Grant-in-Aid for Young Scientists (B)

Allocation TypeSingle-year Grants
Research Field Functional biochemistry
Research InstitutionEhime University (2008)
Osaka Prefecture University (2006-2007)

Principal Investigator

SUGIURA Miwa  Ehime University, 無細胞生命科学工学研究センター, 准教授 (80312255)

Project Period (FY) 2006 – 2008
Keywords生体エネルギー変換 / 光合成電子伝達系 / 酸素発生 / 部位特異的変異 / 好熱性ラン藻
Research Abstract

光合成によるエネルギー変換機能を改良して新規エネルギー生産に応用することを目的として、その初発の反応である水の酸化機能を高めた組換え体の作製を試みた。遺伝子組換えによって遺伝子の発現を制御したり、光化学系II反応中心のアミノ酸を変えることにより、水の酸化機能を1.8倍高めることに成功した。更に機能を高める目的で、水の酸化で生じた電子を受け取る+1.2 Vの高い酸化還元電位を持つP680クロロフィルのリガンドアミノ酸を別のアミノ酸に置換したところ、水の酸化には殆ど影響しないことが明らかになった。

  • Research Products

    (30 results)

All 2009 2008 2007 2006

All Journal Article (20 results) Presentation (9 results) Patent(Industrial Property Rights) (1 results)

  • [Journal Article] Complete EPR spectrum of the S3-state of the oxygen-evolving Photosystem II2009

    • Author(s)
      Boussac, A., Sugiura, M., Rutherford, A.W. and Dorlet, P.
    • Journal Title

      J. Am. Chem. Soc 131

      Pages: 5050-5051

  • [Journal Article] Probing the coupling between proton and electron transfer in Photosystem II core complexes containing a 3-fluorotyrosine2009

    • Author(s)
      Rappaport, F. Boussac, A., Force, D. A., Peloquin, J., Brynda, M., Sugiura, M., Un, U., Britt, R. and Diner, B.
    • Journal Title

      J. Am. Chem. Soc 131

      Pages: 4425-4433

  • [Journal Article] 酸素発生型光合成微生物による光-水素生成、新エネルギーの開発の現状と将来特集2009

    • Author(s)
      杉浦美羽
    • Journal Title

      KEC情報(関西電子工業振興センター発刊) 208

      Pages: 28-31

  • [Journal Article] The role of TyrD in the electron transfer kinetics in Photosystem II2008

    • Author(s)
      Szczepaniak, M., Sugiura, M. and Holzwarth, A.R.
    • Journal Title

      Biochim. Biophys. Acta (Bioenergetics) 1777

      Pages: 1510-1517

  • [Journal Article] Monitoring water reactions during the S-state cycle of the photosynthetic water-oxidizing center : Detection of the DOD bending vibrations by means of Fourier transform infrared spectroscopy2008

    • Author(s)
      Suzuki, H., Sugiura, M. and Noguchi, T.
    • Journal Title

      Biochemistry 47

      Pages: 22024-11030

  • [Journal Article] Analysis of xenon binding to photosystem Ii by X-ray crystallography2008

    • Author(s)
      Murray, J.W., Maghlaoui, K., Kargul, J., Sugiura, M., and Barber, J.
    • Journal Title

      Photosynth Res 98

      Pages: 523-527

  • [Journal Article] Evidence for a stable association of Psb30 (Ycf12) with photosystem II core complex in the cyanobacterium Synechocystis sp. PCC 68032008

    • Author(s)
      Inoue-Kashino, N., Takahashi, T., Ban, A., Sugiura, M., Takahashi, Y., Satoh, K. and Kashino, Y
    • Journal Title

      Photosynth. Res 98

      Pages: 323-335

  • [Journal Article] Quantum efficiency distributions of photo-induced side-pathway donor oxidation at cryogenic temperature in Photosystem II2008

    • Author(s)
      Hughes, J. L., Rutherford, A. W., Sugiura, M. and Krausz, E.
    • Journal Title

      Photosynth. Res 98

      Pages: 199-266

  • [Journal Article] X-ray crystallography identifies two chloride binding sites in the oxygen evolving centre of Photosystem II2008

    • Author(s)
      Murray, J.W, Maghlaoui, K., Kargul, J., Ishida, N., Lai, T.-L., Rutherford, A.W., Sugiura, M., Boussac. A. and Barber, J.
    • Journal Title

      Energy & Environmental Science 1

      Pages: 161-166

  • [Journal Article] Isotopic labelling of Photosystem II in Thermosynechococcus elongatus2008

    • Author(s)
      Boussac, A., Verbavatzand, J.-M., and Sugiura, M.
    • Journal Title

      Photosynth. Res 98

      Pages: 285-292

  • [Journal Article] Biosynthetic Exchange of Bromide for Chloride and Strontium for Calcium in the Photosystem II Oxygen-evolving Enzymes2008

    • Author(s)
      Ishida, N, Sugiura, M., Rappaport, F., Lai, T.-L., Rutherford, A.W., and Boussac, A.
    • Journal Title

      J. Biol. Chem 283

      Pages: 13330-13340

  • [Journal Article] Flash-induced FTIR Difference Spectroscopy Shows No Evidence for Structural Coupling of Bicarbonate to the Oxygen-Evolving Mn cluster in Photosystem II2008

    • Author(s)
      Aoyama, C., Suzuki, H., Sugiura, M. and Noguchi, T.
    • Journal Title

      Biochemistry 47

      Pages: 2760-2765

  • [Journal Article] Influence of Histidine-198 of the D1 subunit on the properties of the primary electron donor, P680, of Photosystem II2008

    • Author(s)
      Sugiura, M., Boussac, A., Noguchi, T. and Rappaport, F.
    • Journal Title

      Thermosynechococcus elongatus Biochim. Biophys. Acta (Bioenergetics) 1777

      Pages: 331-342

  • [Journal Article] Low temperature illuminations of Photosystem II from Thermosynechococcus elongatus by visible and near-infrared lights2008

    • Author(s)
      Boussac, A., Sugiura, M., Lai, T.-L. and Rutherford, A.W.
    • Journal Title

      Philos. Trans. R. Soc. Lond.: B. Biol. Sci. 363

      Pages: 1203-1210

  • [Journal Article] Water molecules coupled to the redox-active tyrosine YD in Photosystem II as detection by FTIR Spectroscopy2007

    • Author(s)
      Takahashi, R., Sugiura, M. and Noguchi, T.
    • Journal Title

      Biochemistry 46

      Pages: 14245-14249

  • [Journal Article] Ycf12 is a core subunit in photosystem II complex2007

    • Author(s)
      Kashino, Y., Ikeda, Y., Ban, A., Inoue-Kashino, N., Satoh, K. and Sugiura, M.
    • Journal Title

      Biochim. Biophys. Acta (Bioenergetics) 1767

      Pages: 1269-1275

  • [Journal Article] A nitrite transporter associated with nitrite uptake by higher plant chloroplasts2007

    • Author(s)
      Sugiura, M, Georgescu, M. N. and Takahashi, M.
    • Journal Title

      Plant Cell Physiol 48

      Pages: 1022-1035

  • [Journal Article] Perturbation of the structure of P680 and the charge distribution on its radical cation in isolated reaction center complexes of photosystem II as revealed by Fourier transform infrared spectroscopy2007

    • Author(s)
      Okubo, T., Sugiura, M., Tomo, T., and Noguchi, T.
    • Journal Title

      Biochemistry 46

      Pages: 4390-4397

  • [Journal Article] Characterization of the tyrosine-Z radical and its environment in the spin-coupled S2TyrZ state of Photosystem II from Thermosynechococcus elongatus2007

    • Author(s)
      Un, S., Boussac, A. and Sugiura, M.
    • Journal Title

      Biochemistry 46

      Pages: 3138-3150

  • [Journal Article] Structural perturbation of the carboxylate ligands to the Mn cluster upon Ca2+/Sr2+ exchange in the S-state cycle of photosynthetic oxygen evolution as studied by flash-induced FTIR difference spectroscopy2006

    • Author(s)
      Suzuki, H., Taguchi, Y., Sugiura, M., Boussac, A. and Noguchi, T.
    • Journal Title

      Biochemistry 45

      Pages: 13454-13464

  • [Presentation] 水の酸化反応におけるD1-His332の役割2009

    • Author(s)
      杉浦美羽、大野陽平、Alain Boussac、Fabrice Rappaport、鈴木博行、野口巧、林秀則
    • Organizer
      第50回日本植物生理学会年会
    • Place of Presentation
      名古屋大学
    • Year and Date
      20090321-24
  • [Presentation] 光化学系IIタンパク質複合体の分子構造の解析の現状2009

    • Author(s)
      杉浦美羽
    • Organizer
      平成20年度愛媛地区分析化学講演会
    • Place of Presentation
      愛媛大学
    • Year and Date
      2009-01-29
  • [Presentation] The water splitting enzyme: recent advances2008

    • Author(s)
      A., Boussac, N., Ishida, T.-L., Lai, A.W,. Rutherford, F., Rappaport, Y., Pushkar, M., Sugiura
    • Organizer
      第46回日本生物物理学会シンポジウム-光合成の新たなる潮流-
    • Place of Presentation
      福岡国際会議場
    • Year and Date
      20081203-05
  • [Presentation] 光化学系IIの構造と機能の解明から応用まで2008

    • Author(s)
      杉浦美羽
    • Organizer
      第46回日本生物物理学会シンポジウム-光合成の新たなる潮流-
    • Place of Presentation
      福岡国際会議場
    • Year and Date
      20081203-05
  • [Presentation] 光化学系IIにおける水の分解メカニズム2008

    • Author(s)
      杉浦美羽
    • Organizer
      2008年度日本化学会中国四国支部会
    • Place of Presentation
      愛媛大学
    • Year and Date
      2008-12-04
  • [Presentation] Effects of P680 ligand substitution in the oxygen-evolving Photosystem II of Thermosynechococcus elongatus2008

    • Author(s)
      Miwa Sugiura, Alain Boussac, Takumi Noguchi, and Fabrice Rappaport
    • Organizer
      Protein Island Matsuyama Inernational Symposium
    • Place of Presentation
      Matsuyama
    • Year and Date
      2008-09-26
  • [Presentation] 酸素発生型光合成微生物による光-水素生成2008

    • Author(s)
      杉浦美羽
    • Organizer
      2008年度KECセミナー新エネルギー開発の現状と将来-更なるCO2削減を目指して-
    • Place of Presentation
      (社)関西電子工業振興センター
    • Year and Date
      2008-07-18
  • [Presentation] Thermosynechococcus elongatus光化学系IIにおけるP680リガンドの酸化還元電位への影響2007

    • Author(s)
      杉浦美羽、Alain Boussac、野口巧、Fabrice Rappaport
    • Organizer
      第48回日本植物生理学会年会
    • Place of Presentation
      愛媛大学
    • Year and Date
      20070328-30
  • [Presentation] 光化学系IIにおける酸素発生の分子機能と構造:部位特異的変異を導入した好熱性シアノバクテリアが解明のブレークスルーになる2007

    • Author(s)
      杉浦美羽
    • Organizer
      第87回日本化学会春期年会特別企画シンポジウム(光合成機能の分子メカニズムと工学応用-分子レベルの探求から太陽光エネルギー変換系への応用まで-)
    • Place of Presentation
      関西大学
    • Year and Date
      2007-03-26
  • [Patent(Industrial Property Rights)] 光合成を利用した酸素発生型光合成微生物による水素生産方法2008

    • Inventor(s)
      杉浦美羽
    • Industrial Property Rights Holder
      杉浦美羽
    • Industrial Property Number
      特願2008-6011 特許権
    • Filing Date
      2008-01-15

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Published: 2010-06-10   Modified: 2016-04-21  

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