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2008 Fiscal Year Final Research Report

X-ray crystallographic analysis of DNA using temperature-control crystallization technique

Research Project

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Project/Area Number 18790030
Research Category

Grant-in-Aid for Young Scientists (B)

Allocation TypeSingle-year Grants
Research Field Physical pharmacy
Research InstitutionKyoto University

Principal Investigator

CHATAKE Toshiyuki  Kyoto University, 原子炉実験所, 准教授 (30383475)

Project Period (FY) 2006 – 2008
Keywords物理化学
Research Abstract

本研究の目的は、独自に開発した温度制御による結晶化法を用いることによりDNA のX 線結晶解析の効率を飛躍的に向上させ、構造に基づいた核酸系薬剤のドラッグデザインへの基盤を確立することにある。今回の研究では、(1)温度制御結晶化法を結晶成長学的な観点から実証して、(2)これに基づいたB 型・Z 型DNA の結晶化と高分解能X 線結晶構造解析を行い、(3)薬剤設計の基本となる特殊なDNA (デオキシ型リボザイム・ミニヘアピンDNA・ボロン化合物を導入したDNA)の結晶化を行った。

  • Research Products

    (9 results)

All 2009 2008 2007

All Journal Article (6 results) (of which Peer Reviewed: 5 results) Presentation (3 results)

  • [Journal Article] Functional aberration of myofibrils by cardiomyopathy-causing mutations in the coiled-coil region of the troponin-core domain2009

    • Author(s)
      Matsumoto F, Maeda K, Chatake T, Maeda Y, Fujiwara S
    • Journal Title

      Biochem BioPhys Research Communication 382

      Pages: 205-209

    • Peer Reviewed
  • [Journal Article] Hydrogen bonds of DsrD protein revealed by neutron crystallography2008

    • Author(s)
      Chatake T, Higuchi Y, Mizuno N, Tanaka I, Niimura N, Morimoto Y
    • Journal Title

      Journal of Synchrotron Radiation 15

      Pages: 277-280

    • Peer Reviewed
  • [Journal Article] An abnormal pK(a) value of internal histidine of the insulin molecule revealed by neutron crystallographic analysis2008

    • Author(s)
      Ishikawa T, Chatake T, Morimoto Y, Maeda M, Kurihara K, Tanaka I, Niimura N
    • Journal Title

      Biochem BioPhys Research Communication 376

      Pages: 32-35

    • Peer Reviewed
  • [Journal Article] Preliminary time-of-flight neutron diffraction study of human deoxyhemoglobin2008

    • Author(s)
      Kovalevsky AY, Chatake T, Shibayama N, Park SY, Ishikawa T, Mustyakimov M, Fisher SZ, Langan P, Morimoto Y
    • Journal Title

      Acta Crystallogr F64

      Pages: 270-273

    • Peer Reviewed
  • [Journal Article] Protonation states of buried histidine residues in human deoxyhemoglobin revealed by neutron crystallography2007

    • Author(s)
      Chatake T, Shibayama N, Park SY, Kurihara K, Tamada T, Tanaka I, Niimura N, Kuroki R, Morimoto Y
    • Journal Title

      Journal of American Chemical Society 129

      Pages: 14840-14841

    • Peer Reviewed
  • [Journal Article] Protocol of refinement in neutron crystallographic analysis2007

    • Author(s)
      Chatake T, Morimoto Y
    • Journal Title

      日本中性子科学会誌 17

      Pages: 216-217

  • [Presentation]2009

    • Author(s)
      茶竹俊行
    • Organizer
      温度制御によるDNA の結晶化とその応用タンパク質結晶育成研究会
    • Place of Presentation
      東海村(茨城県)
    • Year and Date
      2009-03-06
  • [Presentation]2008

    • Author(s)
      Chatake, Ishikawa, Morimoto
    • Organizer
      Crystal Structures of Z-DNA complexed with Ca2+ ion, and Mg2+ ion XXI Congress and General Assembly of the International Union of Crystallography
    • Place of Presentation
      大阪市(大阪府)
    • Year and Date
      2008-08-25
  • [Presentation] 金属イオンがDNA構造に与える影響2007

    • Author(s)
      石川卓哉・森本幸生・茶竹俊行
    • Organizer
      日本結晶学会年会
    • Place of Presentation
      東京都
    • Year and Date
      2007-12-02

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Published: 2010-06-10   Modified: 2016-04-21  

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