黄色猩々蝿のＴＡＬＥモジュレーションを利用した転写因子群の二機能性に関する研究

2019 Fiscal Year Annual Research Report

• Project/Area Number: 18F18792
• Research Institution: Okinawa Institute of Science and Technology Graduate University
• Principal Investigator: ラスカム ニコラス 沖縄科学技術大学院大学, ゲノム・遺伝子制御システム科学ユニット, 教授 (70724087)
• Co-Investigator(Kenkyū-buntansha): CHEN ZHE 沖縄科学技術大学院大学, ゲノム・遺伝子制御システム科学ユニット, 外国人特別研究員
• Project Period (FY): 2018-10-12 – 2020-03-31
• Keywords: GENE REGULATION / STATISTICAL MODELLING / SYSTEMS BIOLOGY / DROSOPHILA MELANOGASTER

Outline of Annual Research Achievements

In FY2019, the fellow’s role was to develop the experimental model to test synthetic regulatory circuits using transcription activator-like (TALE) proteins fused to activator or repressor domains (Crocker et al., 2016). The amount of the TFs contribution is manipulated by adding or subtracting the number of TALE-binding sites in the enhancer. The same approach with TALE fused to Bcd was applied; by adjusting the number of TALE binding sites, the impact of the stepwise increase in Bcd activity will be observed, so testing whether and at what point it switches from repressor to activator. Experimental conditions for generating and assembling essential fragments that compose of transgenic constructs were optimized. They can then be used to create transgenic flies for applications later on. Altogether, the success of these steps provides a strong technical basis and shows great promises for downstream experiments.

Research Progress Status

令和元年度が最終年度であるため、記入しない。

Strategy for Future Research Activity

令和元年度が最終年度であるため、記入しない。