Structural basis of functional coupling between transcription and cellular metabolism

2008 Fiscal Year Self-evaluation Report

• Project/Area Number: 18GS0316
• Research Category: Grant-in-Aid for Creative Scientific Research
• Allocation Type: Single-year Grants
• Research Institution: Osaka University
• Principal Investigator: MORIKAWA Kosuke Osaka University, 蛋白質研究所, 特任研究員(客員教授) (80012665)
• Project Period (FY): 2006 – 2010
• Keywords: 核内受容体 / 脂肪酸代謝物 / 転写共役因子 / 立体構造解析

Research Abstract

「核内受容体の主要な役割は生体内代謝産物をモニタ-し調節することにある」という観点から、転写制御を介した生体恒常性維持機構の解明を目指す。この目標に向けて、X線、NMR、電子顕微鏡による立体構造解析、バイオインフォマティックス、細胞生物学的手法などを駆使し、代謝産物による核内受容体活性化機構の構造基盤、核内受容体が認識するリガンドのカタログ化、転写調節複合体の機能解析および構造解析を行なっている。

Research Products

• [Journal Article] The ubiquitin ligase CHIP acts as an upstream regulator of oncogenic pathways (2009)
  • Author(s): Kajiro, M., Hirota, R., Nakajima, Y., Kawanowa, K., So-ma, K., Ito, I., Yamaguchi, Y., Ohie, S., Kobayashi, Y., Seino, Y., Kawano, M., Kawabe, Y. I., Takei, H., Hayashi, S., Kurosumi, M., Murayama, A., Kimura, K. & Yanagisawa, J.
  • Journal Title: Nature Cell Biol 11 Pages: 312-319
  • Peer Reviewed
• [Journal Article] Atomic structure of mutant PPARγLBD complexed with 15d-PGJ2 : novel modulation mechanism of PPARγ/RXRαfunction by covalently bound ligands (2009)
  • Author(s): Waku, T., Shiraki, T., Oyama, T. & Morikawa, K.
  • Journal Title: FEBS Lett. 583 Pages: 320-324
  • Peer Reviewed
• [Journal Article] Structural insight into PPARγ activation through covalent modification with endogenous fatty acids (2009)
  • Author(s): Waku, T., Shiraki, T., Oyama, T., Fujimoto, Y., Maebara, K., Kamiya, N., Jingami, H. & Morikawa, K.
  • Journal Title: J. Mol. Biol. 385 Pages: 188-199
  • Peer Reviewed
• [Journal Article] Epigenetic control of rDNA loci in response to intracellular energy status (2008)
  • Author(s): Murayama, A., Ohmori, K., Fujimura, A., Minami, H., Yasuzawa-Tanaka, K., Kuroda, T., Oie, S., Daitoku, H., Okuwaki, M., Nagata, K., Fukamizu, A., Kimura, K., Shimizu, T. & Yanagisawa, J.
  • Journal Title: Cell 133 Pages: 627-629
  • Peer Reviewed
• [Presentation] The chromosomal association of condensin II by non-catalytic action of PP2A (2009)
  • Author(s): Kimura, K.
  • Organizer: MEXT Priority Research Project "Cell Proliferation Control" International Symposium
  • Place of Presentation: Nagoya, Japan
  • Year and Date: 2009-02-27
• [Presentation] PPARγ activating process through covalent modification by endogenous fatty acids (2008)
  • Author(s): Shiraki, T.
  • Organizer: Lipid Peroxidation
  • Place of Presentation: Karuizawa, Japan
  • Year and Date: 2008-10-16
• [Presentation] Structural view of PPARγ activating process through covalent modification by endogenous fatty acids XXII Paulo Foundation : Symposium (2008)
  • Author(s): Morikawa, K.
  • Organizer: INPEC
  • Place of Presentation: Naantali Spa, Finland
  • Year and Date: 2008-06-14
• [Presentation] The identification and characterization of a novel nucleolar protein, Nucleomethylin (2007)
  • Author(s): Murayama A.
  • Organizer: 20th Wilhelm Bernhard Workshop
  • Place of Presentation: St. Andrews, UK
  • Year and Date: 2007-08-28
• [Remarks] "The ubiquitin ligase CHIP acts as an upstream regulator of oncogenic Pathways" (Kajiro et.al., Nature Cell Biol., 2009)に関して、平成21年2月9日に読売新聞夕刊に、平成21年2月10日に毎日新聞、日本経済新聞、日刊工業新聞、茨城新聞に掲載。
• [Remarks] Epigenetic Control of rDNA Loci in Response to Intracellular Energy Status (A. Murayama, et.al., Cell,2008)に関して、平成20年5月19日に日経産業新聞に、平成20年5月30日に科学新聞に掲載。