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2010 Fiscal Year Final Research Report

Physico-chemical approach on the construction of functional denovo cell

Research Project

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Project/Area Number 18GS0421
Research Category

Grant-in-Aid for Creative Scientific Research

Allocation TypeSingle-year Grants
Research InstitutionKyoto University

Principal Investigator

YOSHIKAWA Kenichi  京都大学, 大学院・理学研究科, 教授 (80110823)

Co-Investigator(Kenkyū-buntansha) IMANAKA Tadayuki  立命館大学, 生命科学部, 教授 (30029219)
AKIYOSHI Kazunari  京都大学, 工学研究科, 教授 (90201285)
NOMURA Shinichiro  京都大学, 物質-細胞統合システム拠点, 研究員 (50372446)
SETO Hidenori  高エネルギー加速器研究機構, 教授 (60216546)
KITAHATA Hiroyuki  千葉大学, 理学部, 講師 (20378532)
ARUGA Takayuki  東京大学, 工学研究科, 助教 (30452262)
稲垣 紫緒  京都大学, 理学研究科, 研究員 (20452261)
Co-Investigator(Renkei-kenkyūsha) KANAI Tamotsu  京都大学, 工学研究科, 助教 (10346083)
INAGAKI Shio  京都大学, ベンチャー・ビジネス・ラボラトリー, 研究員 (20452261)
SATO Yuko  京都大学, 理学研究科, 研究員 (30423528)
ICHIKAWA Masatoshi  京都大学, 理学研究科, 講師 (40403919)
TAKINOUE Masahiro  東京大学, 生産技術研究所, 特任研究員 (20511249)
IMAMURA Hisako  京都大学, 理学研究科, 研究員 (30523790)
Project Period (FY) 2006 – 2010
Keywordsリポソーム / 人工細胞モデル / 化学物理 / 物性基礎論 / 生物物理 / 自己組織化 / 生命物理 / ゲノムDNA
Research Abstract

1) We have successfully constructed a model experimental system exhibiting robust on/off switching on genetic activity accompanied by the structural transition of a giant DNA molecule. 2) It is shown that micro-confinement causes specific phase-segregation. We demonstrated marked acceleration of gene-expression reaction in an artificial model cell. 3) We proposed a novel theoretical model to explain the emergent process of the morphology in multicellular systems.

  • Research Products

    (17 results)

All 2012 2011 2010 2009 Other

All Journal Article (10 results) (of which Peer Reviewed: 10 results) Presentation (2 results) Book (3 results) Remarks (1 results) Patent(Industrial Property Rights) (1 results)

  • [Journal Article] Cell-Sized confinement in microspheres accelerates the reaction of gene expression2012

    • Author(s)
      A. Kato, M. Yanagisawa, Y. T. Sato, K. Fujiwara, and K. Yoshikawa
    • Journal Title

      Scientific Reports

      Volume: 2 Pages: 283/1-5

    • DOI

      DOI:10.1038/srep00283

    • Peer Reviewed
  • [Journal Article] Critical behavior of megabase-size DNA toward the transition into a compact state2011

    • Author(s)
      Y. Yoshikawa, Y. Suzuki, K. Yamada, W. Fukuda, K. Yoshikawa, K. Takeyasu, T. Imanaka
    • Journal Title

      Journal of Chemical Physics

      Volume: 135(22) Pages: 225101/1-7

    • DOI

      DOI:10.1063/1.3666845

    • Peer Reviewed
  • [Journal Article] Transformation of acto HMM assembly confined in cell-sized liposome2011

    • Author(s)
      K. Takiguchi, M. Negishi, Y. T.-Takiguchi, M. Homma, and K. Yoshikawa
    • Journal Title

      Langmuir

      Volume: 27(18) Pages: 11528-11535

    • DOI

      DOI:10.1021/la2016287

    • Peer Reviewed
  • [Journal Article] Synthesis and in situ insertion of a site-specific fluorescently labeled membrane protein into cell-sized liposomes2011

    • Author(s)
      T. Ohtsuka, S. Neki, T. Kanai, K. Akiyoshi, S. M. Nomura, T. Ohtsuki
    • Journal Title

      Analytical Biochemistry

      Volume: 418 Pages: 97-101

    • DOI

      10.1016/j.ab.2011.06.026

    • Peer Reviewed
  • [Journal Article] DNA compaction in a crowded environment with negatively charged proteins2010

    • Author(s)
      M. K. Krotova, V. V. Vasilevskaya, N. Makita, K. Yoshikawa, and A. R. Khokhlov
    • Journal Title

      Physical Review Letters

      Volume: 105(12) Pages: 128302

    • DOI

      DOI:10.1103/PhysRevLett.105.128302

    • Peer Reviewed
  • [Journal Article] Characteristic effect of an anticancer dinuclear platinum(II) complex on the higher-order structure of DNA2010

    • Author(s)
      N. Kida, Y. Katsuda, Y. Yoshikawa, S. Komeda, T. Sato, Y. Saito, M. Chikuma, M. Suzuki, T. Imanaka, K. Yoshikawa
    • Journal Title

      Journal of Biological Inorganic Chemistry

      Volume: 15(5) Pages: 701-707

    • DOI

      DOI:10.1007/s00775-010-0637-y

    • Peer Reviewed
  • [Journal Article] Rotary motion driven by a direct current electric field2010

    • Author(s)
      M. Takinoue, Yu Atsumi, and K. Yoshikawa
    • Journal Title

      Applied Physics Letters

      Volume: 96(10) Pages: 104105

    • DOI

      DOI:10.1063/1.3358385

    • Peer Reviewed
  • [Journal Article] Direct integration of cell-free synthesized connexin-43 into liposomes utilizing chaperone-like function of lipo-somes2010

    • Author(s)
      Y. Moritani, S. M. Nomura, I. Morita and K. Akiyoshi
    • Journal Title

      FEBS Journal

      Volume: 277 Pages: 3343-332

    • DOI

      DOI:10.1111/j.1742-4658.2010.07736.x

    • Peer Reviewed
  • [Journal Article] Conformational transition of giant DNA in a confined space surrounded by a phospholipid membrane2009

    • Author(s)
      A. Kato, E. Shindo, T. Sakaue, A. Tsuji, and K. Yoshikawa
    • Journal Title

      Biophysical Journal

      Volume: 97(6) Pages: 1678-1686

    • DOI

      DOI:10.1016/j.bpj.2009.06.041

    • Peer Reviewed
  • [Journal Article] Protein synthesis in giant liposomes using the in vitro translation system of The rmococcus kodakaraensis2009

    • Author(s)
      K. Yamaji, T. Kanai, S. M. Nomura, K. Akiyoshi, M. Negishi, Y. Chen, H. Atomi, K. Yoshikawa, T. Imanaka
    • Journal Title

      IEEE Transactions on Nano Bioscience

      Volume: 8(4) Pages: 325-331

    • DOI

      DOI:10.1109/TNB.2009.2035278

    • Peer Reviewed
  • [Presentation] Real-World Modeling on Hierarchical Dynamics of Living Matter2011

    • Author(s)
      K. Yoshikawa
    • Organizer
      International Conference on Hierarchical Structures in Complex Fluids
    • Place of Presentation
      北京、中国
    • Year and Date
      2011-07-05
  • [Presentation] Non-Turing Scenario on Spatio-Temporal Structure Formation2011

    • Author(s)
      K. Yoshikawa
    • Organizer
      7^<th> International Conference on Biological Physics
    • Place of Presentation
      San Diego, USA
    • Year and Date
      2011-06-22
  • [Book] Construction of an In Vitro Model of a Living Cellular System2011

    • Author(s)
      K. Yoshikawa, S. M. Nomura, K. Tsumoto and K. Takiguchi
    • Total Pages
      173-193
    • Publisher
      Springer
  • [Book] 細胞の自己組織化に物理の視点から迫る2011

    • Author(s)
      吉川研一, 岩城貴史
    • Total Pages
      13-21
    • Publisher
      パリティ
  • [Book] Elucidation of Single Molecular Observation of a Giant DNA2010

    • Author(s)
      C. Shew and K. Yoshikawa
    • Total Pages
      207-236
    • Publisher
      World Scientific Publishing Company
  • [Remarks] ホームページ(代表者吉川研一関係)

    • URL

      http://www.chem.scphys.kyoto-u.ac.jp/index_j.html

  • [Patent(Industrial Property Rights)] 定電場によるマイクロサイズの物体の輸送および力学的仕事の取り出し2009

    • Inventor(s)
      吉川研一,瀧ノ上正,浩厚見悠
    • Industrial Property Rights Holder
      (出願人)独立行政法人科学技術振興機構
    • Industrial Property Number
      特許、特許2009-038446号
    • Filing Date
      2009-02-20

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Published: 2013-07-31   Modified: 2015-10-28  

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