Synthesis and Virus Capture of Self-Propelled Protein Microtube Motors by Bubble Jetting of Enzyme Reaction

2020 Fiscal Year Final Research Report

• Project/Area Number: 18H01833
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Review Section: Basic Section 28030:Nanomaterials-related
• Research Institution: Chuo University
• Principal Investigator: KOMATSU Teruyuki 中央大学, 理工学部, 教授 (30298187)
• Co-Investigator(Kenkyū-buntansha): 森田 能次 中央大学, 理工学部, 助教 (40795308)
• Project Period (FY): 2018-04-01 – 2021-03-31
• Keywords: タンパク質 / カタラーゼ / ウレアーゼ / 大腸菌 / ウイルス / 酵素 / 金ナノ粒子

Outline of Final Research Achievements

We have synthesized microtube (MT) motors with a cylindrical hollow structure consisting of proteins. The MT with catalase on the inner surface was self-propelled by jetting oxygen bubbles generated by the disproportionation of hydrogen peroxide. The MT with urease interior surface was self-propelled by ejection of the product generated by the decomposition of urea. Furthermore, the MT motors coated with concanavalin A captured Escherichia coli. The MT motors coated with α-glucosidase acted as a self-stirring catalyst. The MT motor coated with fetuin captured efficiently the spike protein of influenza virus. In addition, the self-propelling velocity of MT coated with gold nanoparticles increased by light irradiation.

Free Research Field

生体高分子化学、マイクロ・ナノマテリアル化学、超分子化学

Academic Significance and Societal Importance of the Research Achievements

酵素反応を駆動力源として自走する蛋白質MTモーターの一群を合成し、それらの機能を明らかにした。得られた成果は、生体適合性に優れた薬物キャリアー、バイオコンテナー、病原体クリーナー、バイオ分子マシンなど、様々な新規生体機能材料の創出につながると考えられる。薬物送達システムの分子設計に新たな指針を与えるばかりでなく、バイオマテリアル化学に新領域を拓くものと期待される。人類の健康・福祉にもたらす波及効果と意義もきわめて大きい。