2020 Fiscal Year Final Research Report

Effects of Transplanted Human Cord Blood-Mononuclear Cells on Pulmonary Remodeling in Small Pulmonary Vascular Bed

Research Project

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Project/Area Number	18H02888
Research Category	Grant-in-Aid for Scientific Research (B)
Allocation Type	Single-year Grants
Section	一般
Review Section	Basic Section 55030:Cardiovascular surgery-related
Research Institution	Shikoku Central Hospital of the Mutual Aid Association of Public School Teachers (2019-2020) The University of Tokushima (2018)
Principal Investigator	Kitagawa Tetsuya 公立学校共済組合四国中央病院(臨床研究センター), 臨床研究センター, 研究員 (80240886)
Co-Investigator(Kenkyū-buntansha)	中川竜二徳島大学, 病院, 講師 (10403685) 北市隆徳島大学, 大学院医歯薬学研究部(医学域), 准教授 (20335813) 加地剛徳島大学, 病院, 准教授 (20467822)
Project Period (FY)	2018-04-01 – 2021-03-31
Keywords	臍帯血 / 細胞移植 / 未熟肺血管床
Outline of Final Research Achievements	Pulmonary hypertension due to congenital heart disease is often a clinical problem. We have found that in monocrotaline (MCT)-induced pulmonary hypertension model mice, transvenous myeloid cell transplants improve pulmonary hypertension. Therefore, in terms of transplant cell collection, we examined whether a more minimally invasive human umbilical cord blood-derived mononuclear cell (hUCB-MNC) transplantation would improve pulmonary hypertension to the same extent as bone marrow stem cell transplant. Research results show that 1) transplanting hUCB-MNCs into an MCT-induced pulmonary hypertension model in immunocompromised mice improves pulmonary hypertension; 2) its effect expression requires cord blood cells themselves rather than serum components; and 3) indicates that the transplant cells staying in lung tissue for a period of time may contribute to the improved effect of the pulmonary vascular bed.
Free Research Field	心臓血管外科学関連
Academic Significance and Societal Importance of the Research Achievements	本研究成果は、これらの基礎動物研究の成果を足がかりとして、独創的な臨床研究「分娩時に採取した患児の自己臍帯血を、分娩後の低肺血流性心臓病の診断確定後に、自発呼吸が開始され肺血流量が増加しはじめた可及的24時間以内に臍帯血細胞移植を行うことで、低容量肺血管床をより発育させる」に進むことを目的としている。我々のこれまでのマウス傷害肺血管床に対する同種骨髄単核球細胞及びヒト臍帯血細胞異種移植による良好な肺血管床再生実績を基盤として、本研究で更に実用的な基礎研究を培い、やがて新しい安価な治療法を開発しようとする点で、独自性と創造性を有するもので、学術的かつ社会的な意義がある。