Development of peptide FET sensor for highly sensitive and simple detection of breast cancer

2022 Fiscal Year Final Research Report

• Project/Area Number: 18K04905
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Review Section: Basic Section 28040:Nanobioscience-related
• Research Institution: Japan Advanced Institute of Science and Technology
• Principal Investigator: Biyani Manish 北陸先端科学技術大学院大学, 共創的国際研究推進本部, 特任教授 (00599780)
• Co-Investigator(Kenkyū-buntansha): 高村 禅 北陸先端科学技術大学院大学, 先端科学技術研究科, 教授 (20290877) ; 山下 啓子 北海道大学, 大学病院, 教授 (70332947)
• Project Period (FY): 2018-04-01 – 2023-03-31
• Keywords: Peptide

Outline of Final Research Achievements

A silicon-based FET sensor was developed to measure the expression of cathepsin E (CatE). This was achieved by immobilizing a CatE-specific substrate peptide on the FET surface and measuring CatE cleavage using a palm-sized portable FET device (called FETSOR) (Fig. 1 left). The measurement conditions for operating the biosensor were optimized at pH 7.4 and 0.01X PBS. As a result, 2.3 pmol of CatE successfully distinguished the presence or absence of activity to cleave the substrate peptide (Fig. 1 right). The total charge on the peptide at pH 7.4 was +8.51, which decreased to +3.61 after cleavage, as confirmed by a 0.05 V decrease in the measured gate voltage. Thus, the usefulness of the FETSOR-based CatE measurement was successfully demonstrated and evaluated. The project will end with the achievement of developing a portable peptide-based FET sensor for the simple and rapid detection of the breast cancer biomarker cathepsin E.

Free Research Field

Biosensor

Academic Significance and Societal Importance of the Research Achievements

FETSORを用いたCatE測定の有用性を実証・評価することに成功した。乳がんバイオマーカーであるカテプシンEを簡便かつ迅速に検出するための携帯型ペプチドベースFETセンサーの開発という成果をもって、本研究課題の完了とする。