Exploratory research of microRNA related to pre-metastatic lymph node niche formation in prostate cancer.

2021 Fiscal Year Final Research Report

• Project/Area Number: 18K07210
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Review Section: Basic Section 50010:Tumor biology-related
• Research Institution: Saitama Medical University (2019-2021); Aichi Medical University (2018)
• Principal Investigator: Kanao Kent 埼玉医科大学, 医学部, 准教授 (20327620)
• Co-Investigator(Kenkyū-buntansha): 都築 豊徳 愛知医科大学, 医学部, 教授 (70627645)
• Project Period (FY): 2018-04-01 – 2022-03-31
• Keywords: 前転移ニッチ / エクソソーム / microRNA / リンパ節転移 / 前立腺癌

Outline of Final Research Achievements

Among patients who underwent total prostatectomy and lymph node dissection, patients with a Gleason score of 6 (Low risk group), patients with 9 or higher (High risk group), and patients with lymph node metastasis (N) (+) Group) resected lymph node FFPE sections were collected. The lymph node tissue of the section (in the N (+) group, the site without metastasis of the lymph node with metastasis) was macrodissection and miRNA-seq was performed. When the FDR value was set to <0.05 and the FDR value was set to Log2 <-0.5 or> 0.5, we found one miRNA between the High risk group and the N (+) group, and 7 miRNA between the High risk group and the N (+) group that showed significant differences in expression.

Free Research Field

泌尿器腫瘍学

Academic Significance and Societal Importance of the Research Achievements

今回有意差を示したmiRNAについては、今後個別に定量PCRでもその差を明らかにしていく予定である。これらのmiRNAは本研究の目的であった、リンパ節の前転移ニッチ形成に関与するmiRNAの候補となる。今後の検証により、前転移ニッチ形成に対する感度、特異度が高いmiRNAであれば、リンパ節転移を起こす準備段階の前立腺癌を診断することが可能となり、前立腺生検検体や血中からのmiRNA抽出により監視療法の適応患者選択や、監視療法中に治療介入を行うかどうかを判断するバイオマーカーの一つに利用できると考えられる。