2019 Fiscal Year Research-status Report
Development of an improved therapy for stroke based on multiple cell types transplantation
| Project/Area Number |
18K07369
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| Research Institution | Shimane University |
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Principal Investigator |
SK.A bdullahMD 島根大学, 学術研究院医学・看護学系, 助教 (30403447)
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| Co-Investigator(Kenkyū-buntansha) |
長井 篤 島根大学, 学術研究院医学・看護学系, 教授 (40273940)
塩田 由利 島根大学, 学術研究院医学・看護学系, 助教 (10581415) [Withdrawn]
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| Project Period (FY) |
2018-04-01 – 2021-03-31
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| Keywords | Stroke, / Multiple cell therapy / Neural stem cell / Microglia / Mesenchymal stem cells |
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| Outline of Annual Research Achievements |
In this study, we aimed to develop a therapy for stroke by transplantation of multiple types of cells. To pursue such research goal, our achievements so far are: 1. we developed and characterized neural stem cell (NSC) line, and optimized condition for microglial and NSC co-transplantation. 2. We developed a rat stroke model by occluding middle cerebral artery (MCAO), and co-transplanted IL-4 primed microglia and NSC line. 3. Evaluation of the pathological changes induced by transplantation of cell types
Following results we have demonstrated: a) IL-4 primed microglia transplantation increased NSC accumulation in stroke area. b) IL-4 primed microglia decreased tissue damage and cell infiltration in stroke area. c) IL-4 primed microglia decreased M1 type, and increased and M2 microglia in stroke area. d) IL-4 primed microglia transplantation increased astrocyte number in stroke core. e) IL-4 primed microglia transplantation increased neuron number in stroke core. f) IL-4 primed microglia transplantation increased vessel number in stroke core.
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| Current Status of Research Progress |
Current Status of Research Progress
2: Research has progressed on the whole more than it was originally planned.
Reason
(A) Currently we are preparing a manuscript based on the results we have got from transplantation of IL-4 primed microglia and NSC transplantation in MCAO rat model for publication in an international scientific journal.
(B) We shall present our data to at 61st Annual Meeting of the Japanese Society of Neurology.
(C) We are planning to prepare a manuscript based on the results of in vitro interaction of M1 type microglia and M2 type microglia with neural stem cells regarding its growth, differentiation and apoptosis.
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| Strategy for Future Research Activity |
To develop further, we are planning to investigate:
(A) Effects of M1 and M2 type mono-cell transplantation on clinical and pathological features of stroke in animal models. The changes will be checked regarding cell infiltration, morphological and expressional changes of infiltrating cells, and reparative processes after stroke.
(B) Effects of mesenchymal stem cell and NSC co-transplantation on clinical and pathological features of stroke in animal models. The changes will be checked regarding cell infiltration, morphological and expressional changes of infiltrating cells, and reparative processes after stroke.
(C) Effects of IL-4 primed microglia, mesenchymal stem cell and NSC co-transplantation on clinical and pathological features of stroke in animal models. The changes will be checked regarding cell infiltration, morphological and expressional changes of infiltrating cells, and reparative processes after stroke.
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| Causes of Carryover |
Reasons for Incurring Amount to be Used Next Fiscal Year and Usage Plan: Last year we had a plan to publish the data of our study in reputed international scientific journals. We decided to publish the study in reputed open access journals so that our findings will be easily distributed widely. Publishing in open access journals require article processing fees. Since, publishing research reports sometimes require long time, unexpectedly we could not published our research reports. Moreover, for unexpected delay of 2018, we could not present our data in scientific meetings and conferences. Since we are preparing our research reports for publication, we are hopeful that the reports will be published this year. We shall also present our findings at 61st Annual Meeting of the Japanese Society of Neurology.
Plan to use the incurring amount in this fiscal year: 1. Publish our findings in reputed international scientific journals 2. Present our findings in scientific meetings and conferences. 3. Use money for the experiments to see the effects of M1 and M2 type mono-cell transplantation on clinical and pathological features of stroke in animal models.
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