2021 Fiscal Year Final Research Report

Search for lipid differentiation markers for safe cartilage regenerative medicine using stem cells and elucidate their functions

Research Project

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Project/Area Number	18K09090
Research Category	Grant-in-Aid for Scientific Research (C)
Allocation Type	Multi-year Fund
Section	一般
Review Section	Basic Section 56020:Orthopedics-related
Research Institution	Hokkaido University
Principal Investigator	BABA RIKIYA 北海道大学, 医学研究院, 客員研究員 (40815742)
Co-Investigator(Kenkyū-buntansha)	岩崎倫政北海道大学, 医学研究院, 教授 (30322803) 小野寺智洋北海道大学, 大学病院, 講師 (70547174)
Project Period (FY)	2018-04-01 – 2022-03-31
Keywords	ヒト誘導多能性幹細胞 / 糖鎖解析
Outline of Final Research Achievements	In this study, we focused on the fact that the surface of undifferentiated iPS cells were coated with specific glycoconjugates and GSL glycans, we established a method to detect and quantify undifferentiated iPS cells in chondrocytes using cell-specific GSL-glycan analysis by applying the Aminolysis-SALSA method. Using quantification method by glycan analysis, we could determine the optimal conditions for use of R-17F antibody, which recognizes specific glycans of iPS cells and has cytotoxic activity. Furthermore, we transplanted the antibody-treated co-cultured cells into SCID mice and showed that no teratoma was formed.
Free Research Field	軟骨再生医療
Academic Significance and Societal Importance of the Research Achievements	GSL糖鎖解析による定量化法は、フローサイトメトリー等で必要となる事前の蛍光標識等の前処置を必要とせず、また心筋細胞等の組織に対する細胞解離の前処理を必要としない。本定量化法はiPS由来生成物の品質管理に有用である可能性がある。未分化iPS細胞の主要な除去方法の一つは、特異的な抗体を用いたcell sortingであるが、iPS由来最終生成物のviabilityを低下させる可能性がある。本研究では、R-17Fを添加するだけで未分化iPS細胞を除去することが可能であり、cell sortingによって起こりうるリスクを回避することができた。