2019 Fiscal Year Research-status Report
Study of plankton community structure by environmental DNA metabarcoding under red soil pollution
|Research Institution||Okinawa Institute of Science and Technology Graduate University |
アレス アンジェラ 沖縄科学技術大学院大学, 海洋生態物理学ユニット, 研究員 (30817549)
|Project Period (FY)
2018-04-01 – 2021-03-31
|Keywords||red soil pollution|
|Outline of Annual Research Achievements
The project entitled “Study of plankton community structure by environmental DNA metabarcoding under red soil pollution” aims to apply DNAbased identification of biodiversity (through DNA metabarcoding) as an approach to evaluate plankton community changes and to identify indicator species of Red Soil Pollution.
During this second year (FY2019) we concluded with part of the data analysis of the project and concluded the first manuscript which is published in the Biorxiv preprint journal (https://www.biorxiv.org/content/10.1101/801886v1.full) and currently submitted to the "Environmental Microbiology journal". The completed tasks from the project during this year included:
1) Data analysis, 2) paper preparation and 3)) manuscript submission
In addition, the results obtained until the date were presented in the international conference: Ocean Sciences Meeting which was held in San Diego California 16th of February to 21st of February of 2020) (https://www.agu.org/Ocean-Sciences-Meeting). I contributed with an Oral Presentation entitled: “Prokaryote Community and Physicochemical Shifts due to Storm Induced Terrestrial Runoff in Subtropical Coastal Ecosystems ” within the session of “Microbial Genomics to Improve Predictive Understanding of Disturbance in the Global Ocean System I ” the 19th of February.
|Current Status of Research Progress
Current Status of Research Progress
2: Research has progressed on the whole more than it was originally planned.
During this second year we could finish the first manuscript from this project entitled: "Extreme storm-induced run-off causes rapid, context-dependent shifts in nearshore subtropical bacterial communities”. We also presented our results in the 2020 Ocean Science conference -one of the most important conference in aquatic science- so an important feedback from other experts in the international community was gained.
Through the analysis of our data, we could obtain insightful data that shows plankton responses under red soil pollution for the first time. As a summary we could experimentally demonstrate that the red soil runoff causes an influx of terrestrial prokaryotes and eukaryotes into the Okinawan coast, but this is rather a fast event since the microbial community returns to the pre-typhoon state after few hours to days. In 2018 the Okinawa typhoon season was very intensive compared with previous years and and included 29 tropical storms, 13 typhoons, and 7 super typhoons( from June to the end of October). This allowed us to collect numerous samples along the season and in addition to the recently finished manuscript that have been submitted to a peer review journal we are working in other 2 publications to publish this data.
|Strategy for Future Research Activity
During this fiscal year FY2020 we have planned to finish with the DNA extraction of some of the extra samples we collected in 2018 to prepare an additional manuscript on metagenomics analysis of prokaryote plankton community dynamics under red soil pollution.
Due to the high amount of data collected during the past typhoon season we are also currently working on a third manuscript. This third manuscript will validate our observations in DNA analysis with the Imaging FlowCytobot (IFCB) -an in-situ automated submersible imaging flow cytometer that generates images of particles in-flow, specially corresponding to phytoplankton-. Therefore, in this second study we will compare both approaches to identify biodiversity. At this moment, we already analysed all the DNA, also determined heavy metal and nutrient concentrations (as described in the first section) and we are currently working on the taxonomic identification from the images captured by the IFCB. To conclude this study we need to still work on the coorelation analysis and prepare the manuscript. It will be submitted before the end of FY
|Causes of Carryover
I will need the remain budget in DNA extraction and sequencing analysis to work on the next study on metagenomics analysis of prokaryote plankton community dynamics under red soil pollution.
The results obtained from this next study will shed light on how coastal ecosystems respond to the red soil pollution problem in Okinawa. This methodology will be applicable to other locations in the world suffering from similar environmental issues.
Research Products (3 results)