2010 Fiscal Year Final Research Report
Production of knockout animals by spermatogonial stem cells
| Project/Area Number |
19201040
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| Research Category |
Grant-in-Aid for Scientific Research (A)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Applied genomics
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| Research Institution | Kyoto University |
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Principal Investigator |
SHINOHARA Takashi Kyoto University, 医学研究科, 教授 (30322770)
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| Project Period (FY) |
2007 – 2010
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| Keywords | 幹細胞 / 生殖 / 遺伝学 |
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| Research Abstract |
Spermatogonial stem cells (SSCs) are the only stem cells in the body with germline potential, which makes them an attractive target for germline modification. We previously showed the feasibility of homologous recombination in mouse SSCs, and produced knockout (KO) mice by exploiting germline stem (GS) cells, i.e., cultured spermatogonia with SSC activity. In this study, we report the successful homologous recombination in rat GS cells, which can be readily established by their ability to form germ cell colonies on culture plates whose surfaces are hydrophilic and neutrally charged and thus limit somatic cell binding. We established a drug selection protocol for GS cells under hypoxia. The frequency of the homologous recombination of the occludin gene was 4.2% (2/48). However, these GS cell lines failed to produce offspring following xenogeneic transplantation into mouse testes and microinsemination, suggesting that long-term culture and drug selection have a negative effect on GS cells. Nevertheless, our results demonstrate the feasibility of gene targeting in rat GS cells and pave the way toward the generation of KO rats.
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[Journal Article] Abnormal DNA methyltransferase expression in mouse germline stem cells results in spermatogenic defects.2009
Author(s)
Takashima S., Takehashi M., Lee J., Chuma S., Okano M., Hata K., Suetake I., Nakatsuji N., Miyoshi H., Tajima S., Tanaka Y., Toyokuni S., Sasaki H., Kanatsu-Shinohara M., Shinohara T.
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Journal Title
Biol.Reprod. 81(1)
Pages: 155-64
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[Journal Article] Production of knockout mice by gene targeting in multipotent germline stem cells.2007
Author(s)
Takehashi M, Kanatsu-Shinohara M, Miki H, Lee J, Kazuki Y, Inoue K, Ogonuki N, Toyokuni S, Oshimura M, Ogura A, Shinohara, T.
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Journal Title
Dev.Biol. 312
Pages: 344-352
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