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2009 Fiscal Year Final Research Report

Elucidation of speciation process of amphidiploid species in Brassica

Research Project

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Project/Area Number 19208001
Research Category

Grant-in-Aid for Scientific Research (A)

Allocation TypeSingle-year Grants
Section一般
Research Field Breeding science
Research InstitutionTohoku University

Principal Investigator

NISHIO Takeshi  Tohoku University, 大学院・農学研究科, 教授 (30301039)

Co-Investigator(Kenkyū-buntansha) KITASHIBA Hiroyasu  東北大学, 大学院・農学研究科, 准教授 (80431542)
TAKAHATA Yoshihito  岩手大学, 農学部, 教授 (10133894)
YOKOI Shuji  岩手大学, 農学部, 准教授 (80346311)
KANEKO Yukio  宇都宮大学, 農学部, 教授 (40241848)
Project Period (FY) 2007 – 2009
Keywords種間不和合性 / 雑種胚崩壊 / 自家不和合性 / ゲノム再配列 / 遺伝子発現制御
Research Abstract

Cooperative research of three laboratories was performed to elucidate the speciation process of amphidiploid species in genus Brassica by investigating genes responsible for interspecific incompatibility and breakdown of hybrid embryos and mechanisms of self-compatibility. We identified QTLs of the strengths of interspecific incompatibility and embryo breakdown, which are clues to identification of the genes. Several S genotypes were found in three amphidiploid species, and mutations of dominant S haplotypes were considered to be the cause of self-compatibility.

  • Research Products

    (20 results)

All 2010 2009 2008 2007

All Journal Article (15 results) (of which Peer Reviewed: 15 results) Presentation (1 results) Book (4 results)

  • [Journal Article] Improvement of the dot-blot-SNP technique for efficient and cost-effective genotyping2010

    • Author(s)
      Shiokai S, Shirasawa K, Sato Y, Nishio T
    • Journal Title

      Mol.Breed. 25

      Pages: 179-186

    • Peer Reviewed
  • [Journal Article] Assessment of genetic diversity of accessions in Brassicaceae genetic resources by frequency distribution analysis of S haplotypes2010

    • Author(s)
      Takuno S, Oikawa E, Kitashiba H, Nishio T
    • Journal Title

      Theor.Appl.Genet. 120

      Pages: 1129-1138

    • Peer Reviewed
  • [Journal Article] Genetic analysis of interspecific incompatibility in Brassica rapa2010

    • Author(s)
      Udagawa H, Ishimaru Y, Li F, Sato Y, Kitashiba H, Nishio T
    • Journal Title

      Theor.Appl.Genet. (印刷中)

    • Peer Reviewed
  • [Journal Article] Simple and efficient methods for S genotyping and S screening in genus Brassica by dot-blot analysis2010

    • Author(s)
      Oikawa E, Takuno S, Izumita A, Sakamoto K, Hanzawa H, Kitashiba H, Nishio T
    • Journal Title

      Mol.Breed. (印刷中)

    • Peer Reviewed
  • [Journal Article] Prediction of the optimum hybridization conditions of dot-blot-SNP analysis using estimated melting temperature of oligonucleotide probes2010

    • Author(s)
      Shiokai S, Kitashiba H, Nishio T
    • Journal Title

      Plant Cell Rep. (印刷中)

    • Peer Reviewed
  • [Journal Article] A Brassica rapa linkage map of EST-based SNP markers for identification of candidate genes controlling flowering time and leaf morphological traits2009

    • Author(s)
      Li F, Kitashiba H, Inaba K, Nishio T
    • Journal Title

      DNA Res. 16

      Pages: 311-323

    • Peer Reviewed
  • [Journal Article] Leaf-punch method to prepare a large number of PCR templates from plants for SNP analysis2009

    • Author(s)
      Shiokai S, Kitashiba H, Shirasawa K, Nagano K, Nishio T
    • Journal Title

      Mol.Breed. 23

      Pages: 329-336

    • Peer Reviewed
  • [Journal Article] Production and characterization of Brassica napus-Raphanus sativus monosomic addition lines mediated by the synthetic amphidiploids "Raphanobrassica"2009

    • Author(s)
      Akaba M, Kaneko Y, Ito Y, Nakata Y, Bang BW, Matsuzawa Y
    • Journal Title

      Breed.Sci. 59

      Pages: 109-118

    • Peer Reviewed
  • [Journal Article] Identification and evaluation of clubroot resistance of radish chromosome using a Brassica napus-Raphanus sativus monosomic addition line2009

    • Author(s)
      Akaba M, Kaneko Y, Hatakeyama K, Ishida M, Bang BW, Matsuzawa Y
    • Journal Title

      Breed.Sci. 59

      Pages: 203-206

    • Peer Reviewed
  • [Journal Article] Preservation of a pseudogene by gene conversion and diversifying selection2008

    • Author(s)
      Takuno S, Nishio T, Satta Y, Innan H
    • Journal Title

      Genetics 180

      Pages: 517-531

    • Peer Reviewed
  • [Journal Article] Hypomethylation and transcriptional reactivation of retrotransposon-like sequences in ddm1 transgenic plants of Brassica rapa2008

    • Author(s)
      Fujimoto R, Sasaki T, Inoue H, Nishio T
    • Journal Title

      Plant Mol.Biol. 66

      Pages: 463-473

    • Peer Reviewed
  • [Journal Article] The pattern of amplification and differentiation of Ty1-copia and Ty3-gypsy retrotransposons in Brassicaceae species2008

    • Author(s)
      Fujimoto R, Takuno S, Sasaki T, Nishio T
    • Journal Title

      Genes Genet.Syst. 83

      Pages: 13-22

    • Peer Reviewed
  • [Journal Article] Genotypic variation for floral characters in Brassica and allied genera with special reference to breeding system2008

    • Author(s)
      Takahata Y, Konno N, Hinata K
    • Journal Title

      Breed.Sci. 58

      Pages: 385-392

    • Peer Reviewed
  • [Journal Article] Effects of recombination on the hitchhiking diversity in Brassica self-incompatibility locus complex2007

    • Author(s)
      Takuno S, Fujimoto R, Sugimura T, Sato K, Okamoto S, Zhang S-L, Nishio T
    • Journal Title

      Genetics 177

      Pages: 949-958

    • Peer Reviewed
  • [Journal Article] Self-compatibility in Brassica napus is caused by independent mutations in S-locus genes2007

    • Author(s)
      Okamoto S, Odashima M, Fujimoto R, Sato Y, Kitashiba H, Nishio T
    • Journal Title

      Plant J. 50

      Pages: 391-400

    • Peer Reviewed
  • [Presentation] Brassica rapa と Raphanus sativus の属間交雑における雑種胚崩壊性のQTL解析2010

    • Author(s)
      殿崎薫・道場和也・北柴大泰・房相佑・金子幸雄・西尾剛
    • Organizer
      日本育種学会
    • Place of Presentation
      京都大学
    • Year and Date
      2010-03-27
  • [Book] Self-incompatibility in Brassicaceae species. In "Biology and breeding of crucifers" Ed.2009

    • Author(s)
      Kitashiba H, Nishio T
    • Total Pages
      99-112
    • Publisher
      By Gupta SK Taylor & Francis Group, LLC
  • [Book] Floral variation in the subtribe Brassicinae with special reference to pollination strategies and pollen-ovule ratios. In "Biology and breeding of crucifers" Ed.2009

    • Author(s)
      Takahata Y
    • Total Pages
      69-78
    • Publisher
      By Gupta SK Taylor & Francis Group, LLC
  • [Book] Distant hybridization. In "Biology and breeding of crucifers" Ed.2009

    • Author(s)
      Kaneko Y, Bang S-W, Matsuzawa Y
    • Total Pages
      207-248
    • Publisher
      By Gupta SK Taylor & Francis Group, LLC
  • [Book] Self-incompatibility. Adv.Bot.Res.2007

    • Author(s)
      Fujimoto R, Nishio T
    • Total Pages
      140-155

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Published: 2011-06-18   Modified: 2016-04-21  

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