2010 Fiscal Year Final Research Report
Field trial of transgenic poplars for industrial use
| Project/Area Number |
19208016
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| Research Category |
Grant-in-Aid for Scientific Research (A)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
林産科学・木質工学
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| Research Institution | Tokyo University of Agriculture (2009-2010)
Kyoto University (2007-2008) |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
谷口 亨 森林総合研究所, 森林バイオ研究センター, 研究員 (00360470)
馬場 啓一 京都大学, 生存圏研究所, 助教 (20238223)
海田 るみ 東京農業大学, 応用生物科学部, 研究員 (00398885)
太田 誠一 京都大学, 大学院・農学研究科, 教授 (10346033)
磯貝 明 東京大学, 大学院・農学生命研究科, 教授 (40191879)
舘野 正樹 東京大学, 理学研究科, 准教授 (00179730)
吉田 正人 名古屋大学, 生命農学研究科, 准教授 (30242845)
富田 裕子 (半場 裕子) 京都工芸繊維大学, 学内共同利用センター, 准教授 (90314666)
古田 裕三 京都府立大学, 生命環境科学科, 准教授 (60343406)
菊池 彰 筑波大学, 大学院・生命環境科学研究科, 講師 (00400648)
西窪 伸之 独立行政法人理化学研究所, 植物科学センター, 研究員 (30392062)
荒川 圭太 北海道大学, 大学院・農学研究科, 准教授 (00241381)
加来 友美 京都大学, 生存圏研究所, 研究員 (10378911)
朴 龍又 京都大学, 生存圏研究所, 研究員 (00378910)
栗田 学 独立行政法人林木育種センター, 研究員 (40370829)
坪村 美代子 独立行政法人林木育種センター, 研究員 (70415040)
渡邊 和男 筑波大学, 大学院・生命環境科学研究科, 教授 (90291806)
間野 絵梨子 京都大学, 生存圏研究所, 研究員 (60447935)
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| Project Period (FY) |
2007 – 2010
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| Keywords | 組織構造 / 材形成 |
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| Research Abstract |
The transgenic expression of Aspergillus xyloglucanase cDNA(AaXEG2) with 35S promoter in the leaves of open-field grown poplars was studied. This level was slightly decreased in the poplars grown in fertile soil for four years. Xyloglucan content was greatly decreased in the walls of the tissues in the transgenic lines, although the degradation levels of xyloglucan were slightly lower for the poplars grown in fertile soil than for those grown in non-fertile soil. The leaves exhibited a smaller surface area with more rounded teeth than did those of the wild type plants, as if they were of the sun leaf variety that was grown in the incubation room and subsequently greenhoused. Large numbers of proteins were markedly downregulated in the leaves of the transgenic plants via proteomic analysis. Significant reduction occurred not only on the moduli of elasticity and rupture in both their live stems and dried trunks compared with those in the wild type.
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[Journal Article] Plant fiber formation : state of the art, recent and expected progress and open questions2011
Author(s)
Gorshkova T, Brutch N, Chabbert B, Deyholos M, Hayashi T, Lev-Yadun S, Mellerowicz EJ, Morvan C, Neutelings G, Pilate G
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Journal Title
Critical Reviews in Plant Sciences
Volume: 31
Pages: 201-228
Peer Reviewed
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[Journal Article] Potential role for purple acid phosphatase in the dephosphorylation of wall proteins in tobacco cells2010
Author(s)
Kaida, R, Serada, S, Norioka, N, Norioka, S, Neumetzler, L, Pauly, M, Sampedro, J, Zarra, I, Hayashi, T, Kaneko, TS
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Journal Title
Plant Physiol
Volume: 153
Pages: 603-610
Peer Reviewed
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[Journal Article] Xyloglucan for generating tensile stress to bend tree stem2009
Author(s)
Baba K, Park WP, Kaku T, Kaida R, Takeuchi M, Yoshida M, Hosoo Y, Ojio Y, Okuyama T, Taniguchi T, Ohmiya Y, Kondo T, Shani J, Shoseyov O, Awano T, Serada S, Norioka N, Norioka S, Hayashi T
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Journal Title
Mol Plant
Volume: 2
Pages: 893-903
Peer Reviewed
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[Journal Article] Structural organization and a standardized nomenclature for plant endo-1, 4-β-glucanases(cellulases) of glycosyl hydrolase family 92007
Author(s)
BR Urbanowicz, AB Bennett, E del Campillo, C Catala, T Hayashi, B Henrissat, H Hofte, SJ McQueen-Mason, SE Patterson, O Shoseyov, TT Teeri and JKC Rose
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Journal Title
Plant Physiology
Volume: 144
Pages: 1693-1696
Peer Reviewed
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[Journal Article] Analyses of leaves from open field-grown transgenic poplars overexpressing xyloglucanase
Author(s)
Kaku T, Baba K, Taniguchi T, Kurita M, Konagaya K, Ishii K, Kondo T, Serada S, Iizuka H, Kaida R, Taji T, Sakata Y, Hayashi T
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Journal Title
DOI
Peer Reviewed
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